Cloned (Comment) | Organism |
---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Comment) | Organism |
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nickel-NTA column | Salmonella enterica subsp. enterica serovar Typhimurium |
the apoenzyme of OASS-B is prepared using hydroxylamine as the resolving reagent. Apoenzyme is reconstituted to holoenzyme by addition of pyridoxal 5'-phosphate | Salmonella enterica subsp. enterica serovar Typhimurium |
using Ni-NTA chromatography | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
apo-enzyme reconstituted with cofactor pyridoxal 5'-phosphate exhibits 81.4% enzyme activity compared to the native enzyme, 100 mM HEPES, pH 7.0, with 0.2 mM O3-acetyl-L-serine, and 0.05 5-thio-2-nitrobenzoate | Salmonella enterica subsp. enterica serovar Typhimurium |
Storage Stability | Organism |
---|---|
-80°C, 5 mM HEPES, pH 8.0 | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-thio-2-nitrobenzoate + O-acetyl-L-serine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | acetate + ? | - |
? | |
O3-acetyl-L-serine + 5-thio-2-nitrobenzoate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ? + acetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
O-acetylserine sulfhydrylase-B | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
OASS | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
OASS-B | produced under unaerobic grwoth conditions in contrast to OASS-A | Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
assay performed at room temperature | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | the 31P chemical shift of the internal and external Schiff bases of pyridoxal 5'-phosphate in OASS-B are further downfield compared to OASS-A, suggesting a tighter binding of the cofactor in the B-isozyme. The chemical shift of the internal Schiff base (ISB) of OASS-B is 6.2 ppm, the highest value reported for the ISB of a PLP-dependent enzyme. Considering the similarity in the binding sites of the pyridoxal 5'-phosphate cofactor for both isozymes, torsional strain of the C5-C5' bond (O4'-C5'-C5-C4) of the Schiff base is proposed to contribute to the further downfield shift | Salmonella enterica subsp. enterica serovar Typhimurium | |
pyridoxal 5'-phosphate | tighter binding than in OASS-A, treatment with 1 mM hydroxylamine in 500 mM phosphate, pH 7.6, with 0.2 mM dithiothreitol, followed by dialysis against 100 mM phosphate, pH 7.0, with 0.2 mM dithiothreitol gerates apo-enzyme without the cofactor | Salmonella enterica subsp. enterica serovar Typhimurium |