Cloned (Comment) | Organism |
---|---|
expression in with plasmid pRSM40 in Escherichia coli NM522 | Salmonella enterica subsp. enterica serovar Typhimurium |
Protein Variants | Comment | Organism |
---|---|---|
H152A | shift in the ketoenamine to enolimine tautomeric equilibrium toward the neutral enolimineine, the internal Schiff base of the free enzyme, the amino acid external Schiff base, and the alpha-aminoacrylate intermediate. The decreased rate of the mutant likely reflects a decrease in the amount of active enzyme as a result of an increased flexibility of the cofactor , which leads to increased rates of interconversion of the open and closed forms of the enzyme and additional interactions between the cofactor and enzyme in the closed form of the enzyme. Analysis of spectral properties | Salmonella enterica subsp. enterica serovar Typhimurium |
H152A | shift in the ketoeneamine to enolimine tautomeric equilibrium towards neutral enolimine in the internal Shiff base of the free enzyme, the amino acid external Schiff base, and the alpha-aminoacrylate intermediate, 2 enzyme conformers are present, decreased rate of the enzyme likely reflects a decrease in the amount of active enzyme as result of the increased cofactor flexibility which stabilizes the nonproductive binding of O3-acetyl-L-serine in the external Schiff base | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
O-acetyl-L-serine | mutant H52A, pH 6.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.11 | - |
O3-acetyl-L-serine | H152A mutant with 136fold decrease compared to wild type, 100 mM MES, pH 6.5, 50 microM 5-thio-2-nitrobenzoate (TNB), 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.6 | - |
5-thio-2-nitrobenzoate | wild-type, pH 7.0 | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.6 | - |
5-thio-2-nitrobenzoate | wild type, 100 mM HEPES, pH 7.0, 0.5 mM O3-acetyl-L-serine, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.5 | - |
5-thio-2-nitrobenzoate | mutant H52A, pH 6.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.5 | - |
5-thio-2-nitrobenzoate | H152A mutant with 4.1fold increase compared to wild type, 100 mM MES, pH 6.5, 0.5 mM O3-acetyl-L-serine, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
15 | - |
O-acetyl-L-serine | wild-type, pH 7.0 | Salmonella enterica subsp. enterica serovar Typhimurium | |
15 | - |
O3-acetyl-L-serine | wild type, 100 mM HEPES, pH 7.0, 50 microM 5-thio-2-nitrobenzoate (TNB), 25°C | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O3-acetyl-L-serine + hydrogen sulfide | Salmonella enterica subsp. enterica serovar Typhimurium | - |
L-cysteine + acetate | - |
? | |
O3-acetyl-L-serine + hydrogen sulfide | Salmonella enterica subsp. enterica serovar Typhimurium DW378 | - |
L-cysteine + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
DW378 | - |
Salmonella enterica subsp. enterica serovar Typhimurium DW378 | - |
DW378 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-serine + 5-thio-2-nitrobenzoate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ? + acetate | - |
? | |
O-acetyl-L-serine + 5-thio-2-nitrobenzoate | - |
Salmonella enterica subsp. enterica serovar Typhimurium DW378 | ? + acetate | - |
? | |
O3-acetyl-L-serine + 5-thio-2-nitrobenzoate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ? + acetate | - |
? | |
O3-acetyl-L-serine + 5-thio-2-nitrobenzoate | - |
Salmonella enterica subsp. enterica serovar Typhimurium DW378 | ? + acetate | - |
? | |
O3-acetyl-L-serine + hydrogen sulfide | - |
Salmonella enterica subsp. enterica serovar Typhimurium | L-cysteine + acetate | - |
? | |
O3-acetyl-L-serine + hydrogen sulfide | - |
Salmonella enterica subsp. enterica serovar Typhimurium DW378 | L-cysteine + acetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
O-acetylserine sulfhydrylase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
OASS-A | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | wild type and mutant exhibit almost identical values for sulfide as substrate, imidazole has no effect on the activity of wild type or H152A mutant enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.06 | - |
O3-acetyl-L-serine | the turnover for H152A mutant is decreased 9fold compared to the wild type, 25°C, 100 mM MES, pH 6.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.56 | - |
O3-acetyl-L-serine | 25°C, 100 mM HEPES, pH 7.0 | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH-rate profiles for wild type and H152A mutant with O3-acetyl-L-serine and 5-thio-2-nitrobenzoate as substrates are similar, 100 mM concentrations of 2-(N-morpholino)ethanesulfonic acid (MES), pH 5.5-6.5, 3-(N-morpholino)propanesulfonic acid (MOPS), pH 7.0-7.5, N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid (HEPES), pH 8.0, and 3-[[tris(hydroxymethyl)]amino]propanesulfonic acid (TAPS), pH 9.0, titrated with KOH | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | the 5'-phosphate is tightly bound to the enzyme via a hydrogen bond to His152, one to the residues responsible for positioning of the cofactor | Salmonella enterica subsp. enterica serovar Typhimurium |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | wild type and mutant exhibit almost identical values for sulfide as substrate | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.0219 | - |
5-thio-2-nitrobenzoate | H152A mutant with 43fold decrease compared to wild type, 100 mM MES, pH 6.5, 0.5 mM O3-acetyl-L-serine, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.037 | - |
O3-acetyl-L-serine | wild type, 100 mM HEPES, pH 7.0, 50 microM 5-thio-2-nitrobenzoate (TNB), 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.51 | - |
O3-acetyl-L-serine | H152A mutant with 14fold increase compared to wild type, 100 mM MES, pH 6.5, 50 microM 5-thio-2-nitrobenzoate (TNB), 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.95 | - |
5-thio-2-nitrobenzoate | wild type, 100 mM HEPES, pH 7.0, 0.5 mM O3-acetyl-L-serine, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium |