Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.47 extracted from

  • Omura, H.; Kuroda, M.; Kobayashi, M.; Shimizu, S.; Yoshida, T.; Nagasawa, T.
    Purification, characterization and gene cloning of thermostable O-acetyl-L-serine sulfhydrylase forming b-cyano-L-alanine (2003), J. Biosci. Bioeng., 95, 470-475.
    View publication on PubMed
Search for more literature for 2.5.1.47

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 L-cysteine pH 7.5, 45°C Geobacillus stearothermophilus
16
-
 O3-acetyl-L-serine pH 7.5, 45°C Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 2 * 34000, SDS-PAGE Geobacillus stearothermophilus
70000
-
 gel filtration Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus Q84IF9
-
-

Purification (Commentary)

Purification (Comment) Organism
ethanol precipitation, ammonium sulfate precipitation, preparative electrophoresis, phenyl sepharose Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cysteine + cyanide
-
 Geobacillus stearothermophilus cyanoalanine + H2S
-
 ?
additional information no substrate: L-serine, L-homoserine, O-acetyl-L-homoserine, L-alanine, L-homocysteine Geobacillus stearothermophilus ?
-
 ?
O3-acetyl-L-serine + benzylmercaptan
-
 Geobacillus stearothermophilus S-benzyl-L-cysteine + acetate
-
 ?
O3-acetyl-L-serine + cyanide
-
 Geobacillus stearothermophilus beta-cyano-L-alanine + acetate
-
 ?
O3-acetyl-L-serine + ethylmercaptan
-
 Geobacillus stearothermophilus S-ethyl-L-cysteine + acetate
-
 ?
O3-acetyl-L-serine + hydrogen sulfide 970% of the activity with cyanide Geobacillus stearothermophilus L-cysteine + acetate
-
 ?
O3-acetyl-L-serine + methylmercaptan
-
 Geobacillus stearothermophilus S-methyl-L-cysteine + acetate
-
 ?
O3-acetyl-L-serine + phenol
-
 Geobacillus stearothermophilus O-phenyl-L-serine + acetate
-
 ?
O3-acetyl-L-serine + phenylmercaptan
-
 Geobacillus stearothermophilus S-phenyl-L-cysteine + acetate
-
 ?
O3-acetyl-L-serine + propylmercaptan
-
 Geobacillus stearothermophilus S-propyl-L-cysteine + acetate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 34000, SDS-PAGE Geobacillus stearothermophilus

Synonyms

Synonyms Comment Organism
beta-cyano-L-alanine synthase
-
 Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 30 min, stable in pH-range of 6.0-10.0 Geobacillus stearothermophilus
70
-
 30 min, pH 7.5, stable Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Geobacillus stearothermophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10
-
 Geobacillus stearothermophilus