General Stability | Organism |
---|---|
stable to several freeze-thaw cycles | Methanosarcina thermophila |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
O-acetylserine | substrate inhibition | Methanosarcina thermophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
H2S | 40°C | Methanosarcina thermophila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
2 * or 3 * 36000, SDS-PAGE | Methanosarcina thermophila |
36000 | - |
3 or 2 * 36000, SDS-PAGE | Methanosarcina thermophila |
93000 | - |
gel filtration | Methanosarcina thermophila |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-Ser + sulfide | Methanosarcina thermophila | final step in Cys synthesis | L-Cys + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina thermophila | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanosarcina thermophila |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.129 | - |
- |
Methanosarcina thermophila |
Storage Stability | Organism |
---|---|
-20 C, complete loss of activity after 2 months | Methanosarcina thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-Ser + H2S | - |
Methanosarcina thermophila | L-Cys + acetate | - |
? | |
O-acetyl-L-Ser + sulfide | final step in Cys synthesis | Methanosarcina thermophila | L-Cys + acetate | - |
? | |
O-acetyl-L-serine | - |
Methanosarcina thermophila | L-cysteine + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * or 3 * 36000, SDS-PAGE | Methanosarcina thermophila |
trimer | 3 or 2 * 36000, SDS-PAGE | Methanosarcina thermophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | - |
Methanosarcina thermophila |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
21 | - |
pH 8.0, 50 mM dithiothreitol, 0.08 mM pyridoxal phosphate, 30 min, stable | Methanosarcina thermophila |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | enzyme contains pyridoxal phosphate | Methanosarcina thermophila |