KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
H2S | pH 8.0, 30°C | Brassica juncea | |
2.5 | - |
O-acetyl-L-Ser | pH 8.0, 30°C | Brassica juncea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
2 * 26000, SDS-PAGE | Brassica juncea |
52000 | - |
gel filtration | Brassica juncea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica juncea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Brassica juncea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Brassica juncea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
410 | - |
- |
Brassica juncea |
Storage Stability | Organism |
---|---|
0 C, potassium phosphate buffer, pH 8, mercaptoethanol, EDTA, three months, no loss of activity | Brassica juncea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | beta-substituted alanines, low activity | Brassica juncea | ? | - |
? | |
O-acetyl-L-Ser + 2-propene-1-thiol | 18% of activity with sulfide | Brassica juncea | S-allyl-L-cysteine + ? | - |
? | |
O-acetyl-L-Ser + H2S | - |
Brassica juncea | L-Cys + acetate | - |
? | |
O-acetyl-L-Ser + mercaptoacetic acid | 6.1% of activity with sulfide | Brassica juncea | S-carboxymethyl-L-cysteine | - |
? | |
O-acetyl-L-Ser + NaCN | 12.3% of activity with sulfide | Brassica juncea | beta-cyanoalanine + sodium acetate | - |
? | |
O-acetyl-L-serine | - |
Brassica juncea | L-cysteine + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 26000, SDS-PAGE | Brassica juncea |
More | amino acid composition | Brassica juncea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Brassica juncea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | one per subunit | Brassica juncea |