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Literature summary for 2.5.1.47 extracted from

  • Yanase, H.; Sakai, T.; Tonomura, K.
    Some properties of a beta-cyanoalanine-forming enzyme of Enterobacter sp. 10-1 (1982), Agric. Biol. Chem., 46, 363-369.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 14% inhibition at 1 mM Enterobacter sp.
Cd2+ 55% inhibition at 1 mM Enterobacter sp.
Cl- HgCl2 Enterobacter sp.
Co2+ complete inhibition at 1 mM Enterobacter sp.
hydroxylamine 31% inhibition at 1 mM Enterobacter sp.
Ni2+ complete inhibition at 1 mM Enterobacter sp.
p-chloromercuribenzoate 40% inhibition at 1 mM Enterobacter sp.
p-chloromercuriphenylsulfonic acid 46% inhibition at 1 mM Enterobacter sp.
Sodium borohydride 59% inhibition at 1 mM Enterobacter sp.
Thiourea 34% inhibition at 1 mM Enterobacter sp.
Zn2+ 95% inhibition at 1 mM Enterobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
H2S pH 7.4, 30°C Enterobacter sp.
5.2
-
NaCN pH 7.4, 30°C Enterobacter sp.
6.7 8.3 O-acetyl-L-Ser pH 7.4, 30°C, cosubstrate-dependent Enterobacter sp.

Organism

Organism UniProt Comment Textmining
Enterobacter sp.
-
-
-
Enterobacter sp. 10-1
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O-acetyl-L-Ser + NaCN
-
Enterobacter sp. beta-cyanoalanine + sodium acetate
-
?
O-acetyl-L-Ser + NaCN
-
Enterobacter sp. 10-1 beta-cyanoalanine + sodium acetate
-
?
O-acetyl-L-serine
-
Enterobacter sp. L-cysteine + acetate
-
?
O-acetyl-L-serine
-
Enterobacter sp. 10-1 L-cysteine + acetate
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
10 min, unstable above Enterobacter sp.
60
-
10 min, no loss of activity Enterobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.75
-
-
Enterobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
8 10
-
Enterobacter sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 37°C, 60 min, stable Enterobacter sp.

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate 0.75 mol per mol subunit Enterobacter sp.