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Literature summary for 2.5.1.47 extracted from
- Some properties of cysteine synthase from radish roots (1976), Agric. Biol. Chem., 40, 637-638.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| relatively stable | Raphanus sativus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| hydroxylamine | - |
Raphanus sativus |  |
| L-homoserine | - |
Raphanus sativus | |
| methionine | slight inhibition | Raphanus sativus | |
| S-methylcysteine | slight inhibition | Raphanus sativus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.17 | - |
O-acetyl-L-Ser | - |
Raphanus sativus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
2 * 33000, SDS-PAGE | Raphanus sativus |
| 66000 | - |
gel filtration | Raphanus sativus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O-acetyl-L-Ser + sulfide | Raphanus sativus | final step in Cys synthesis | L-Cys + acetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Raphanus sativus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Raphanus sativus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | - |
Raphanus sativus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1024 | - |
- |
Raphanus sativus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O-acetyl-L-Ser + sulfide | final step in Cys synthesis | Raphanus sativus | L-Cys + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 33000, SDS-PAGE | Raphanus sativus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
3 min, stable | Raphanus sativus |
| 80 | - |
3 min, unstable | Raphanus sativus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Raphanus sativus | |
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