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Literature summary for 2.5.1.46 extracted from
- A new non-radioactive deoxyhypusine synthase assay adaptable to high throughput screening (2017), Amino Acids, 49, 1793-1804 .
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | DHS activity can be determined by coupling the first phase reaction with the NADH-Glo assay in which the generation of luminescence is dependent on NADH derived from the DHS partial reaction. The non-radioactive DHS/NADH-Glo coupled assay is highly specific, sensitive and reproducible and can be configured for high throughput screening of small molecule libraries | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N''-guanyl-1,7-diaminoheptane | i.e. GC7, almost complete inhibition at 20 microM in both one-step and two-step assays | Homo sapiens |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P49366 | - |
- |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DHS | - |
Homo sapiens |
| DHYS | - |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9.5 | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | there is a measurable release of enzyme-bound NADH in the absence of eIF5A precursor. The NADH:DHS ratio is around 6 | Homo sapiens | |
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Release 2023.1 (January 2023)
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