Literature summary for 2.5.1.46 extracted from

Martinez-Rocha, A.L.; Woriedh, M.; Chemnitz, J.; Willingmann, P.; Kroeger, C.; Hadeler, B.; Hauber, J.; Schaefer, W.
Posttranslational hypusination of the eukaryotic translation initiation factor-5A regulates Fusarium graminearum virulence (2016), Sci. Rep., 6, 24698.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene DHS, targeted enzyme overexpression in Fusarium graminearum by single crossover event inserting the linearized vector pMW-DHS	Fusarium graminearum

Protein Variants

Protein Variants	Comment	Organism
additional information	replacement of the endogenous DHS and DOHH genes by homologous recombination with the PgpdA-DHS or Pgpd1-DOHH alleles, excluding any possibility of mutagenic effects by ectopic integration	Fusarium graminearum

Inhibitors

Inhibitors	Comment	Organism	Structure
CNI-1493	a compound that inhibits fungal DHS activity without affecting grain development	Fusarium graminearum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
[eIF5A-precursor]-lysine + spermidine	Fusarium graminearum	hypusination of Lys56	[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine	-	?

Organism

Organism	UniProt	Comment	Textmining
Fusarium graminearum	D5L6Z2	i.e. Gibberella zeae, gene DHS	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
[eIF5A-precursor]-lysine + spermidine	hypusination of Lys56	Fusarium graminearum	[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine	-	?

Synonyms

Synonyms	Comment	Organism
DHS	-	Fusarium graminearum

General Information

General Information	Comment	Organism
malfunction	importance of imbalances in eIF5A hypusination in Fusarium graminearum: overexpression of gene DHS leads to increased virulence in Triticum aestivum, elevated production of the mycotoxin deoxynivalenol, more infection structures, faster Triticum aestivum tissue invasion in plants and increases vegetatively produced conidia. Overexpression of the enzyme DOHH completely prevents infection structure formation, pathogenicity in Triticum aestivum and Zea mays, leads to overproduction of reactive oxygen species, reduced DON mycotoxin production and increased sexual reproduction. Simultaneous overexpression of both genes restores wild type-like phenotypes, strongly increased hypusinated eIF5A in DOHH overexpression mutant in comparison to wild-type, and the DHS overexpression mutants	Fusarium graminearum
physiological function	activation of eukaryotic translation initiation factor eIF5A requires a posttranslational modification, forming the unique amino acid hypusine. This activation is mediated by two enzymes, deoxyhypusine synthase, DHS, and deoxyhypusine hydroxylase, DOHH. Enzyme DHS catalyzes eIF5A Lys56 hypusination and is essential in Fusarium graminearum	Fusarium graminearum

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Release 2023.1 (January 2023)