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Literature summary for 2.5.1.46 extracted from

  • Njuguna, J.T.; von Koschitzky, I.; Gerhardt, H.; Laemmerhofer, M.; Choucry, A.; Pink, M.; Schmitz-Spahnke, S.; Bakheit, M.A.; Strube, C.; Kaiser, A.
    Target evaluation of deoxyhypusine synthase from Theileria parva the neglected animal parasite and its relationship to Plasmodium (2014), Bioorg. Med. Chem., 22, 4338-4346.
    View publication on PubMed
Search for more literature for 2.5.1.46

Cloned(Commentary)

Cloned (Comment) Organism
gene DHS, located on chromosome 2, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of soluble His-tagged enzyme in Escherichia coli strain Rosetta (DE3) Theileria parva

Inhibitors

Inhibitors Comment Organism Structure
(4-[[2-(1H-benzimidazol-2-yl)hydrazinylidene]methyl]phenyl)(hydroxy)oxoammonium 55% inhibition Theileria parva
8-fluoro-N-[3-(morpholin-4-yl)propyl]-5H-pyrimido[5,4-b]indol-4-amine 23.6% inhibition Theileria parva
additional information inhibitor screning in an anti-malaria compound collection, a guanyl residue seems to be an important lead structure for inhibition of Theileria parva DHS enzyme Theileria parva
N-(7-chloroquinolin-4-yl)-N'-methylpropane-1,3-diamine 12.45% inhibition Theileria parva

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.37
-
 spermidine pH 9.5, 37°C, recombinant enzyme Theileria parva

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
-
 Theileria parva
41260
-
-
 Theileria parva

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[eIF5A-precursor]-lysine + spermidine Theileria parva
-
 [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?
[eIF5A-precursor]-lysine + spermidine Theileria parva Muguga
-
 [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?

Organism

Organism UniProt Comment Textmining
Theileria parva Q4UDC3 gene dhs
-
Theileria parva Muguga Q4UDC3 gene dhs
-

Purification (Commentary)

Purification (Comment) Organism
recombinant soluble His-tagged enzyme from Escherichia coli strain Rosetta (DE3) Theileria parva

Source Tissue

Source Tissue Comment Organism Textmining
schizont significantly increased enzyme expression level Theileria parva
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[eIF5A-precursor]-lysine + spermidine
-
 Theileria parva [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?
[eIF5A-precursor]-lysine + spermidine
-
 Theileria parva Muguga [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 41000, recombinant enzyme, SDS-PAGE, 4 * 41260, about, sequence calculation Theileria parva

Synonyms

Synonyms Comment Organism
DHS
-
 Theileria parva

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Theileria parva

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
 assay at Theileria parva

pI Value

Organism Comment pI Value Maximum pI Value
Theileria parva sequence calculation
-
 5.62

General Information

General Information Comment Organism
metabolism first enzyme of the hypusine pathway Theileria parva
additional information structural comparison of the three-dimensional structure of Theileria parva DHS with Homo sapiens DHS Theileria parva