Literature summary for 2.5.1.46 extracted from

Jakus, J.; Wolff, E.C.; Park, M.H.; Folk, J.E.
Features of the spermidine-binding site of deoxyhypusine synthase as derived from inhibition studies. Effective inhibition by bis- and mono-guanylated diamines and polyamines (1993), J. Biol. Chem., 268, 13151-13159.

Inhibitors

Inhibitors	Comment	Organism
1,3-diaminopropane	-	Rattus norvegicus
1,7-Diaminoheptane	-	Rattus norvegicus
1,8-diaminooctane	-	Rattus norvegicus
1-(3-aminopropyl)-4-aminomethylpiperidine	1 mM, less than 50% inhibition	Rattus norvegicus
1-aminooctane	1 mM, less than 50% inhibition	Rattus norvegicus
1-methylspermidine	0.184 mM, 50% inhibition	Rattus norvegicus
6,6'-difluorospermidine	0.014 mM, 50% inhibition	Rattus norvegicus
6-fluorospermidine	0.048 mM, 50% inhibition	Rattus norvegicus
7,7-Difluorospermidine	1 mM, less than 50% inhibition	Rattus norvegicus
caldine	-	Rattus norvegicus
guazatine	-	Rattus norvegicus
hirudonine	-	Rattus norvegicus
N,N'-bis-benzyldiaminooctane	1 mM, less than 50% inhibition	Rattus norvegicus
N-(2-cyanoethyl)-1,3-diaminopropane	1 mM, less than 50% inhibition	Rattus norvegicus
N-(3-aminopropyl)-1,3-diaminopropane	1 mM, less than 50% inhibition	Rattus norvegicus
N-(3-aminopropyl)-cadaverine	-	Rattus norvegicus
N-(3-aminopropyl)-cis-1,4-diaminocyclohexane	1 mM, less than 50% inhibition	Rattus norvegicus
N-(3-aminopropyl)-trans-1,4-diaminocyclohexane	1 mM, less than 50% inhibition	Rattus norvegicus
N-(3-cyanopropyl)-1,3-diaminopropane	1 mM, less than 50% inhibition	Rattus norvegicus
N-butyl-1,3-diaminopropane	1 mM, less than 50% inhibition	Rattus norvegicus
N1,N3-bis-guanyl-1,3-diaminopropane	1 mM, less than 50% inhibition	Rattus norvegicus
N1,N3-bis-tert-butyloxycarbonylspermidine	1 mM, less than 50% inhibition	Rattus norvegicus
N1,N6-bis-guanyl-1,6-diaminohexane	-	Rattus norvegicus
N1,N7-bis-allylcaldine	1 mM, less than 50% inhibition	Rattus norvegicus
N1,N7-bis-benzylcaldine	1 mM, less than 50% inhibition	Rattus norvegicus
N1,N7-bis-dimethylcaldine	1 mM, less than 50% inhibition	Rattus norvegicus
N1,N7-bis-guanyl-1,7-diaminoheptane	-	Rattus norvegicus
N1,N7-bis-guanylcaldine	-	Rattus norvegicus
N1,N8-bis-guanyl-1,8-diaminooctane	-	Rattus norvegicus
N1-acetylspermidine	1 mM, less than 50% inhibition	Rattus norvegicus
N1-ethylspermidine	1 mM, less than 50% inhibition	Rattus norvegicus
N1-guanyl-1,3-diaminopropane	1 mM, less than 50% inhibition	Rattus norvegicus
N1-guanyl-1,7-diaminoheptane	-	Rattus norvegicus
N1-guanyl-1,8-diaminooctane	-	Rattus norvegicus
N1-guanylcaldine	-	Rattus norvegicus
N1-guanylspermine	-	Rattus norvegicus
N3-ethylspermidine	1 mM, less than 50% inhibition	Rattus norvegicus
N4-(3-aminopropyl)-4-methylpentane-1,4-diamine	1 mM, less than 50% inhibition	Rattus norvegicus
N4-acetylspermidine	1 mM, less than 50% inhibition	Rattus norvegicus
N4-benzoylspermidine	1 mM, less than 50% inhibition	Rattus norvegicus
N4-benzylspermidine	1 mM, less than 50% inhibition	Rattus norvegicus
N4-bromoacetylspermidine	1 mM, less than 50% inhibition	Rattus norvegicus
N4-methylcaldine	1 mM, less than 50% inhibition	Rattus norvegicus
putrescine	1 mM, less than 50% inhibition	Rattus norvegicus
spermidine	-	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
testis	-	Rattus norvegicus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
[eIF5A-precursor]-lysine + spermidine	-	Rattus norvegicus	[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine	i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine	?

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Rattus norvegicus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism
0.0000097	-	N1-guanyl-1,7-diaminoheptane	-	Rattus norvegicus
0.00024	-	N1-guanyl-1,8-diaminooctane	-	Rattus norvegicus
0.00033	-	N1-guanylspermine	-	Rattus norvegicus
0.00074	-	N1-guanylcaldine	-	Rattus norvegicus
0.0017	-	N1,N7-bis-guanyl-1,7-diaminoheptane	-	Rattus norvegicus
0.00489	-	hirudonine	-	Rattus norvegicus
0.00565	-	N1,N8-bis-guanyl-1,8-diaminooctane	-	Rattus norvegicus
0.012	-	guazatine	-	Rattus norvegicus
0.035	-	N1,N6-bis-guanyl-1,6-diaminohexane	-	Rattus norvegicus
0.1545	-	N1,N7-bis-guanylcaldine	-	Rattus norvegicus