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Literature summary for 2.5.1.46 extracted from
- Identification of YHR068w in Saccharomyces cerevisiae chromosome VIII as a gene for deoxyhypusine synthase. Expression and characterization of the enzyme (1995), J. Biol. Chem., 270, 18408-18412.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0083 | - |
spermidine | yeast recombinant eIF-5Aa | Saccharomyces cerevisiae |  |
| 0.0125 | - |
spermidine | yeast recombinant eIF-5Ab | Saccharomyces cerevisiae | |
| 0.56 | - |
NAD+ | yeast recombinant eIF-5Aa | Saccharomyces cerevisiae | |
| 0.81 | - |
NAD+ | yeast recombinant eIF-5Ab | Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Saccharomyces cerevisiae | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
4 * 43000, SDS-PAGE | Saccharomyces cerevisiae |
| 165000 | 172000 | gel filtration sedimentation equilibrium centrifugation | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0131 | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| spermidine | reaction in absence of eIF5A-precursor | Saccharomyces cerevisiae | 1,3-diaminopropane + DELTA1-pyrroline | - |
? | |
| [eIF5A-precursor]-lysine + spermidine | - |
Saccharomyces cerevisiae | [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine | i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 43000, SDS-PAGE | Saccharomyces cerevisiae |
| tetramer | 4* 42998, MALDI mass spectrometry | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Saccharomyces cerevisiae | |
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