Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, the deduced amino acid sequence shows a high degree of identity to that of yeast enzyme and to the known sequences of tryptic peptides from the rat and Neurospora enzymes | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | variant recombinant proteins with i. a truncation of 48 or 97 NH2-terminal amino acids, ii. A truncation of 39 COOH-terminal amino acids, or iii. An internal deletion (ASp262-Ser317) are inactive. A chimeric protein consisting of the complete human sequence and 16 amino acids of the yeast sequence, Gln197-Asn212, not present in the human enzyme, inserted between Glu193 and Gln194 exhibit moderate activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
[eIF5A-precursor]-L-lysine | - |
Homo sapiens | |
0.0048 | - |
NAD+ | - |
Homo sapiens | |
0.0072 | - |
spermidine | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
x * 41000, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[eIF5A-precursor]-lysine + spermidine | Homo sapiens | first step in the biosynthesis of hypusine | [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P49366 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
recombinant enzyme | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.015 | - |
- |
Homo sapiens |
0.019 | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
spermidine | - |
Homo sapiens | 1,3-diaminopropane + DELTA1-pyrroline | - |
? | |
[eIF5A-precursor]-L-lysine + spermidine | - |
Homo sapiens | [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine | - |
? | |
[eIF5A-precursor]-lysine + spermidine | - |
Homo sapiens | [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine | i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine | ? | |
[eIF5A-precursor]-lysine + spermidine | first step in the biosynthesis of hypusine | Homo sapiens | [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 41000, SDS-PAGE | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | strict requirement | Homo sapiens | |
NADH | - |
Homo sapiens |