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Literature summary for 2.5.1.44 extracted from
- Purification and characterization of homospermidine synthase in Acinetobacter tartarogenes ATCC 31105 (1993), J. Biochem., 114, 45-49.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | preincubation, 1 mM, 30 min prior to assay causes 50% inhibition | Acinetobacter tartarogenes |  |
| N-ethylmaleimide | preincubation, 1 mM, 30 min prior to assay causes 91% inhibition | Acinetobacter tartarogenes | |
| NADH | Ki: 0.006 mM | Acinetobacter tartarogenes | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.28 | - |
putrescine | - |
Acinetobacter tartarogenes | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | required for full activity, optimal activity at 50 mM | Acinetobacter tartarogenes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
2 * 52000, SDS-PAGE | Acinetobacter tartarogenes |
| 102000 | - |
gel filtration | Acinetobacter tartarogenes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 putrescine | Acinetobacter tartarogenes | - |
sym-homospermidine + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter tartarogenes | - |
ATCC 31105 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acinetobacter tartarogenes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.41 | - |
- |
Acinetobacter tartarogenes |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 1°C, 20 mM, Tris/HCl, pH 7.5, 10 mM 2-mercaptoethanol, 0.5 mM putrescine, 0.02% NaN3 | Acinetobacter tartarogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 putrescine | - |
Acinetobacter tartarogenes | sym-homospermidine + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 52000, SDS-PAGE | Acinetobacter tartarogenes |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Acinetobacter tartarogenes |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Acinetobacter tartarogenes | |
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