Literature summary for 2.5.1.43 extracted from

Yang, G.; Li, J.; Liu, W.; Yu, Z.; Shi, Y.; Lv, B.; Wang, B.; Han, D.
Molecular cloning and characterization of MxNAS2, a gene encoding nicotianamine synthase in Malus xiaojinensis, with functions in tolerance to iron stress and misshapen flower in transgenic tobacco (2015), Sci. Hortic., 183, 77-86.

No PubMed abstract available

Cloned(Commentary)

Cloned (Comment)	Organism
gene MxNAS2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time PCR expression analysis, recombinant expression in transgenic Nicotiana tabacum cv. Xanthi under control of the CaMV 35S promoter, recombinant isozyme MxNAS2 promotes the synthesis of NAS and increases nicotinamine and chlorophyll contents in the tobacco cells. Overexpression of MxNAS2 improves the tolerance to Fe stress in transgenic tobacco, but leads to delayed flowering. Higher levels of MxNAS2 expression in transgenic tobacco contribute to misshapen flowers and increased levels of Fe, Mn, Cu and Zn in leaf and flower. A MxNAS2-GFP fusion protein is targeted into vesicles and cytoplasmic membrane	Malus baccata

Cloned (Comment)

Organism

gene MxNAS2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time PCR expression analysis, recombinant expression in transgenic Nicotiana tabacum cv. Xanthi under control of the CaMV 35S promoter, recombinant isozyme MxNAS2 promotes the synthesis of NAS and increases nicotinamine and chlorophyll contents in the tobacco cells. Overexpression of MxNAS2 improves the tolerance to Fe stress in transgenic tobacco, but leads to delayed flowering. Higher levels of MxNAS2 expression in transgenic tobacco contribute to misshapen flowers and increased levels of Fe, Mn, Cu and Zn in leaf and flower. A MxNAS2-GFP fusion protein is targeted into vesicles and cytoplasmic membrane

Malus baccata

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	isozyme MxNAS2 is preferentially localized in vesicles and cytoplasmic membrane	Malus baccata	16020	-
vesicle	isozyme MxNAS2 is preferentially localized in vesicles and cytoplasmic membrane	Malus baccata	31982	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
36200	-	x * 36200, about, sequence calculation	Malus baccata

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3 S-adenosyl-L-methionine	Malus baccata	-	3 S-methyl-5'-thioadenosine + nicotianamine	-	?

Organism

Organism	UniProt	Comment	Textmining
Malus baccata	A3DUI9	var. xiaojinensis, gene MxNAS2	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Malus baccata	-
phloem	-	Malus baccata	-
root	-	Malus baccata	-
seedling	-	Malus baccata	-
xylem	low enzyme levels	Malus baccata	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3 S-adenosyl-L-methionine	-	Malus baccata	3 S-methyl-5'-thioadenosine + nicotianamine	-	?

Subunits

Subunits	Comment	Organism
?	x * 36200, about, sequence calculation	Malus baccata

Synonyms

Synonyms	Comment	Organism
MxNAS2	-	Malus baccata

Cofactor

Cofactor	Comment	Organism	Structure
S-adenosyl-L-methionine	-	Malus baccata

pI Value

Organism	Comment	pI Value Maximum	pI Value
Malus baccata	sequence calculation	-	5.18

Expression

Organism	Comment	Expression
Malus baccata	the expression of MxNAS2 is highly affected by Fe stress and indoleacetic acid treatment, whereas, weakly affected by abscisic acid treatment in seedlings	down

General Information

General Information	Comment	Organism
physiological function	the enzyme is involved in nicotinamine biosynthesis. In addition to its role in metal transport in plants, nicotinamine may be involved in the regulation of metal transfer within cells. These results suggest that nicotinamine excess influences the functions of metal-requiring proteins, including some of the transcription factors	Malus baccata