Literature summary for 2.5.1.43 extracted from

Nozoye, T.; Nagasaka, S.; Bashir, K.; Takahashi, M.; Kobayashi, T.; Nakanishi, H.; Nishizawa, N.K.
Nicotianamine synthase 2 localizes to the vesicles of iron-deficient rice roots, and its mutation in the YXXphi or LL motif causes the disruption of vesicle formation or movement in rice (2014), Plant J., 77, 246-260.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene OsNAS2, recombinant expression of GFP-tagged isozyme NAS2 in transgenic Oryza sativa plants under the control of its own promoter. The recombinant GFP-tagged enzyme moves moving dynamically within root cells, phhenotype, ooverview	Oryza sativa

Protein Variants

Protein Variants	Comment	Organism
L115A/L116A	isozyme OsNAS2 mutated in the LL motif resulting in mutant m7-sGFP, which does not localize to the vesicles. Mutant m7sGFP does not show NAS enzyme activity	Oryza sativa
additional information	mutant enzyme phenotype under both Fe-sufficient and -deficient conditions, overview	Oryza sativa
Y105A	isozyme OsNAS2 mutated in the YXXphi motif resulting in mutant m6-sGFP, which is localized to the vesicles. These vesicles stuck together and are immobile. Mutant m6-sGFP converts S-adenosyl methionine into nicotinamine in vitro	Oryza sativa

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
vesicle	-	Oryza sativa	31982	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3 S-adenosyl-L-methionine	Oryza sativa	-	3 S-methyl-5'-thioadenosine + nicotianamine	-	?

Organism

Organism	UniProt	Comment	Textmining
Oryza sativa	Q10MI9	gene OsNAS2	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
root	-	Oryza sativa	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3 S-adenosyl-L-methionine	-	Oryza sativa	3 S-methyl-5'-thioadenosine + nicotianamine	-	?

Synonyms

Synonyms	Comment	Organism
NA synthase 2	-	Oryza sativa
NAS2	-	Oryza sativa
nicotianamine synthase 2	-	Oryza sativa

Cofactor

Cofactor	Comment	Organism	Structure
S-adenosyl-L-methionine	-	Oryza sativa

General Information

General Information	Comment	Organism
additional information	tyrosine motif and a di-leucine motif mutants phenotype under both Fe-sufficient and -deficient conditions, overview. Mutant m6-sGFP converts S-adenosyl methionine into nicotinamine in vitro, whereas mutant m7sGFP does not show NAS enzyme activity	Oryza sativa
physiological function	graminaceous plants release mugineic acid family phytosiderophores (MAs) to acquire iron from the soil. Deoxymugineic acid secretion from rice roots fluctuates throughout the day, and vesicles accumulate in roots before mugineic acid family phytosiderophores secretion. These vesicles are involved in nicotinamine and 2'-deoxymugineic acid biosynthesis. A tyrosine motif and a di-leucine motif, which have been reported to be involved in cellular transport, are conserved in NAS proteins in plants. The localization of enzyme NAS to vesicles and the transport of these vesicles are crucial steps in nicotinamine synthesis, leading to deoxymugineic acid synthesis and secretion in rice. The tyrosine motif is involved in vesicle movement, whereas the di-leucine motif appears to be involved in vesicle localization and OsNAS2 activity, which are crucial for the proper function of OsNAS2	Oryza sativa

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Release 2023.1 (January 2023)