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Literature summary for 2.5.1.43 extracted from

  • Dreyfus, C.; Larrouy, M.; Cavelier, F.; Martinez, J.; Pignol, D.; Arnoux, P.
    The crystallographic structure of thermoNicotianamine synthase with a synthetic reaction intermediate highlights the sequential processing mechanism (2011), Chem. Commun. (Camb. ), 47, 5825-5827.
    View publication on PubMed
Search for more literature for 2.5.1.43

Crystallization (Commentary)

Crystallization (Comment) Organism
purified NAS mutant E81Q complexed with both substrates, S-adenosyl-L-methionine and/or glutamate, or with reaction intermediate N-(3-amino-3-carboxypropyl)glutamic acid, X-ray diffraction structure determination and analysis Methanothermobacter thermautotrophicus

Protein Variants

Protein Variants Comment Organism
E81Q site-directed mutagenesis, inactive mutant enzyme Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 S-adenosyl-L-methionine + glutamate Methanothermobacter thermautotrophicus
-
 2 S-methyl-5'-thioadenosine + thermoNicotianamine + 2 H+ the archaeal product analogue thermoNicotianamine differs from nicotianamine in the carboxy azetidine moiety of nicotianamine that is replaced by a glutamate moiety in thermoNicotianamine, overview ?

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
3 S-adenosyl-L-methionine = 3 S-methyl-5'-thioadenosine + nicotianamine reaction mechanism of the archaeal enzyme, overview. The polymerization mechanism occurs at a single active site with stepwise translocation of the reaction intermediate N-(3-amino-3-carboxypropyl)glutamic acid Methanothermobacter thermautotrophicus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl-L-methionine + glutamate
-
 Methanothermobacter thermautotrophicus 2 S-methyl-5'-thioadenosine + thermoNicotianamine + 2 H+ the archaeal product analogue thermoNicotianamine differs from nicotianamine in the carboxy azetidine moiety of nicotianamine that is replaced by a glutamate moiety in thermoNicotianamine, overview ?
2 S-adenosyl-L-methionine + glutamate substrate binding structures, overview Methanothermobacter thermautotrophicus 2 S-methyl-5'-thioadenosine + thermoNicotianamine + 2 H+ the archaeal product analogue thermoNicotianamine differs from nicotianamine in the carboxy azetidine moiety of nicotianamine that is replaced by a glutamate moiety in thermoNicotianamine, overview ?
additional information the enzyme condenses three aminopropyl moieties of S-adenosylmethionine and the autocyclization of one moiety leads to the formation of an azetidine ring Methanothermobacter thermautotrophicus ?
-
 ?

Synonyms

Synonyms Comment Organism
NAS
-
 Methanothermobacter thermautotrophicus

General Information

General Information Comment Organism
additional information S-adenosyl-L-methionine and glutamate substrate binding structures, overview Methanothermobacter thermautotrophicus