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Literature summary for 2.5.1.41 extracted from
- Purification and characterization of geranylgeranylglyceryl phosphate synthase from a thermoacidophilic archaeon, Thermoplasma acidophilum (2003), J. Biochem., 133, 651-657.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | required for enzymatic activity, optimal concentration: 0.01% | Thermoplasma acidophilum |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinantly expressed in Escherichia coli | Thermoplasma acidophilum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Thermoplasma acidophilum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000075 | - |
geranylgeranyl diphosphate | - |
Thermoplasma acidophilum | |
| 0.0212 | - |
sn-glycerol 1-phosphate | - |
Thermoplasma acidophilum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | optimal concentration 5-10 mM | Thermoplasma acidophilum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
SDS-PAGE | Thermoplasma acidophilum |
| 50000 | - |
gel filtration | Thermoplasma acidophilum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoplasma acidophilum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using DEAE-cellulose column chromatography, NH4SO4 precipitation, Phenyl-650S and Resourse Q columns, Mini Q column and Superdex 200 HR 10/30 column | Thermoplasma acidophilum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geranylgeranyl diphosphate + sn-glycerol 1-phosphate | - |
Thermoplasma acidophilum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | gel filtration, 2 * 27000 | Thermoplasma acidophilum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| geranylgeranylglyceryl phosphate synthase | - |
Thermoplasma acidophilum |
| GGGP synthase | - |
Thermoplasma acidophilum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
assay at and optimal temperature | Thermoplasma acidophilum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at and optimal pH | Thermoplasma acidophilum |
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Release 2023.1 (January 2023)
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