BRENDA - Enzyme Database
show all sequences of 2.5.1.39

Functional characterization of OsPPT1, which encodes p-hydroxybenzoate polyprenyltransferase involved in ubiquinone biosynthesis in Oryza sativa

Ohara, K.; Yamamoto, K.; Hamamoto, M.; Sasaki, K.; Yazaki, K.; Plant Cell Physiol. 47, 581-590 (2006)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Saccharomyces cerevisiae
Oryza sativa
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
complete inhibition at 10 mM
Oryza sativa
Co2+
38.7% of activity remaining at 10 mM
Oryza sativa
Cu2+
complete inhibition at 10 mM
Oryza sativa
Fe2+
28.4% of activity remaining at 10 mM
Oryza sativa
Mn2+
50.3% of activity remaining at 10 mM
Oryza sativa
Ni2+
3.15% of activity remaining at 10 mM
Oryza sativa
Zn2+
complete inhibition at 10 mM
Oryza sativa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00604
-
4-hydroxybenzoate
-
Oryza sativa
0.0597
-
geranyl diphosphate
-
Oryza sativa
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
highest enzyme activity with 10 mM Mg2+
Oryza sativa
Organism
Organism
UniProt
Commentary
Textmining
Oryza sativa
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
farnesyl diphosphate + 4-hydroxybenzoate
-
676467
Oryza sativa
3-farnesyl-4-hydroxybenzoate + diphosphate
-
-
-
?
geranyl diphosphate + 4-hydroxybenzoate
-
676467
Oryza sativa
diphosphate + 3-geranyl-4-hydroxybenzoate
-
-
-
?
geranylgeranyl diphosphate + 4-hydroxybenzoate
-
676467
Oryza sativa
3-geranylgeranyl-4-hydroxybenzoate + diphosphate
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
OsPPT1a
-
Oryza sativa
p-hydroxybenzoate polyprenyltransferase
-
Oryza sativa
PPT
-
Oryza sativa
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Saccharomyces cerevisiae
Oryza sativa
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
complete inhibition at 10 mM
Oryza sativa
Co2+
38.7% of activity remaining at 10 mM
Oryza sativa
Cu2+
complete inhibition at 10 mM
Oryza sativa
Fe2+
28.4% of activity remaining at 10 mM
Oryza sativa
Mn2+
50.3% of activity remaining at 10 mM
Oryza sativa
Ni2+
3.15% of activity remaining at 10 mM
Oryza sativa
Zn2+
complete inhibition at 10 mM
Oryza sativa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00604
-
4-hydroxybenzoate
-
Oryza sativa
0.0597
-
geranyl diphosphate
-
Oryza sativa
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
highest enzyme activity with 10 mM Mg2+
Oryza sativa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
farnesyl diphosphate + 4-hydroxybenzoate
-
676467
Oryza sativa
3-farnesyl-4-hydroxybenzoate + diphosphate
-
-
-
?
geranyl diphosphate + 4-hydroxybenzoate
-
676467
Oryza sativa
diphosphate + 3-geranyl-4-hydroxybenzoate
-
-
-
?
geranylgeranyl diphosphate + 4-hydroxybenzoate
-
676467
Oryza sativa
3-geranylgeranyl-4-hydroxybenzoate + diphosphate
-
-
-
?
Other publictions for EC 2.5.1.39
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
759945
Liu
SmPPT, a 4-hydroxybenzoate po ...
Salvia miltiorrhiza
Plant Cell Rep.
38
1527-1540
2019
-
-
1
-
-
-
-
-
1
-
1
-
-
11
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
2
2
-
-
-
760056
Liu
-
Site-directed mutagenesis of ...
Elizabethkingia meningoseptica
Proc. Biochem.
58
186-192
2017
-
-
-
-
7
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
728802
Cheng
Structural insights into ubiqu ...
Aeropyrum pernix, Aeropyrum pernix DSM 11879
Science
343
878-881
2014
-
-
-
1
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
738168
Scalais
Early myoclonic epilepsy, hype ...
Homo sapiens
Eur. J. Paediatr. Neurol.
17
625-630
2013
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
5
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
723550
Quinzii
Effects of inhibiting CoQ10 bi ...
Homo sapiens
PLoS ONE
7
e30606
2012
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
723246
Forsman
4-Nitrobenzoate inhibits coenz ...
Mus musculus
Nat. Chem. Biol.
6
515-517
2010
-
-
-
-
-
-
1
-
1
-
-
1
-
3
-
-
-
-
-
2
-
-
3
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
703208
Bräuer
A structural model of the memb ...
Escherichia coli
Chembiochem
9
982-992
2008
-
-
1
-
5
-
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
2
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
5
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
703609
Quinzii
Respiratory chain dysfunction ...
Homo sapiens
FASEB J.
22
1874-1885
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
674978
Zhang
Phenotypes and fed-batch ferme ...
Schizosaccharomyces pombe, Schizosaccharomyces pombe SPQ-01
J. Biotechnol.
128
120-131
2007
-
-
-
-
-
-
-
-
2
-
-
-
-
7
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675069
Mollet
Prenyldiphosphate synthase, su ...
Homo sapiens
J. Clin. Invest.
117
765-772
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687033
Lopez-Martin
Missense mutation of the COQ2 ...
Saccharomyces cerevisiae, Homo sapiens
Hum. Mol. Genet.
16
1091-1097
2007
-
1
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
705592
Tran
Endogenous synthesis of coenzy ...
Arabidopsis thaliana, Oryza sativa, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Rattus norvegicus
Mitochondrion
7
62-71
2007
-
-
-
-
-
-
-
-
2
1
-
-
-
5
-
-
-
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
738464
Diomedi-Camassei
COQ2 nephropathy: a newly desc ...
Homo sapiens
J. Am. Soc. Nephrol.
18
2773-2780
2007
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
671299
Buron
Stimulation of polyprenyl 4-hy ...
Homo sapiens
Anal. Biochem.
353
15-21
2006
4
-
-
-
-
-
1
-
1
-
-
-
-
3
-
-
-
-
-
2
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676467
Ohara
Functional characterization of ...
Oryza sativa
Plant Cell Physiol.
47
581-590
2006
-
-
1
-
-
-
7
2
-
1
-
-
-
6
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
7
-
2
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657846
Forsgren
Isolation and functional expre ...
Saccharomyces cerevisiae, Homo sapiens
Biochem. J.
382
519-526
2004
-
-
2
-
1
-
-
-
2
-
-
6
-
2
-
-
-
-
-
6
2
-
9
1
2
2
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
2
-
-
6
-
-
-
-
-
6
2
-
9
1
2
-
-
-
2
-
-
-
-
-
-
-
-
-
660199
Okada
The AtPPT1 gene encoding 4-hyd ...
Arabidopsis thaliana
Plant Mol. Biol.
55
567-577
2004
-
-
1
-
1
-
-
-
1
-
-
2
-
6
-
-
-
-
-
9
-
-
4
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
9
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
705223
Brauer
Modeling the E. coli 4-hydroxy ...
Escherichia coli
J. Mol. Model.
10
317-327
2004
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
1
-
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637004
Tekle
Ubiquinone biosynthesis in rat ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
291
1128-1133
2002
-
-
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
637002
Boehm
Active expression of the ubiA ...
Escherichia coli
Transgenic Res.
9
477-486
2000
-
-
1
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
637005
Uchida
Phenotypes of fission yeast de ...
Schizosaccharomyces pombe
J. Bacteriol.
182
6933-6939
2000
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637006
Wessjohann
-
Prenylation of benzoic acid de ...
Escherichia coli
Angew. Chem.
35
1697-1699
1996
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
703082
Olsson
-
Enzymes of the mevalonate path ...
Rattus norvegicus
Carcinogenesis
6
599-605
1995
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
705488
Andersson
Age-dependent modifications in ...
Rattus norvegicus
Mech. Ageing Dev.
85
1-14
1995
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
637003
Melzer
Characterization of polyprenyl ...
Escherichia coli
Biochim. Biophys. Acta
1212
93-102
1994
1
-
1
-
-
-
4
3
1
3
-
1
-
5
-
-
-
-
-
-
3
-
5
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
1
-
-
-
-
-
4
-
3
1
3
-
1
-
-
-
-
-
-
3
-
5
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
702725
Suzuki
Evidence that Escherichia coli ...
Escherichia coli
Biosci. Biotechnol. Biochem.
58
1814-18149
1994
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
1
1
-
-
704366
Swiezewska
-
Biosynthesis of ubiquinone and ...
Spinacia oleracea
J. Biol. Chem.
15
1494-1499
1993
-
-
-
-
-
-
-
-
4
4
-
-
-
1
-
-
-
-
-
1
-
-
1
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
4
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
704915
Wu
Mutants of Escherichia coli af ...
Escherichia coli
J. Gen. Microbiol.
139
1795-805
1993
-
-
-
-
-
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644233
Nichols
Cloning and sequencing of Esch ...
Escherichia coli
J. Bacteriol.
174
5309-5316
1992
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637000
Kawahara
-
Partial purification and chara ...
Pseudomonas putida
Agric. Biol. Chem.
55
2307-2311
1991
1
-
-
-
-
-
5
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
1
-
-
1
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
637001
Uchida
-
Solubilization of 4-hydroxyben ...
Pseudomonas putida, Pseudomonas putida IAM 1219
Agric. Biol. Chem.
55
2299-2305
1991
5
-
-
-
-
-
12
2
1
1
-
-
-
2
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
1
-
-
-
-
-
-
5
-
-
-
-
-
-
-
12
-
2
1
1
-
-
-
-
-
-
-
-
1
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
636997
Kalen
Nonaprenyl-4-hydroxybenzoate t ...
Rattus norvegicus
J. Biol. Chem.
265
1158-1164
1990
-
-
-
-
-
-
7
-
6
-
-
1
-
4
-
-
-
-
-
5
-
-
3
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
6
-
-
1
-
-
-
-
-
5
-
-
3
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
636998
Gupta
4-Hydroxybenzoate polyprenyltr ...
Rattus norvegicus
Methods Enzymol.
110
327-334
1985
5
-
-
-
-
1
14
2
2
2
-
1
-
3
-
-
-
-
-
4
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
5
-
-
-
-
-
1
-
14
-
2
2
2
-
1
-
-
-
-
-
4
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
636999
Nishino
Effects of detergents on the p ...
Cavia porcellus, Rattus norvegicus
Biochemistry
16
605-609
1977
10
-
-
-
-
-
-
-
4
2
-
-
-
6
-
-
-
-
-
4
-
2
6
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
4
2
-
-
-
-
-
-
-
4
-
2
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
704240
Young
Biochemical and genetic studie ...
Escherichia coli
J. Bacteriol.
110
18-25
1972
-
-
-
-
-
-
5
-
1
4
-
-
-
1
-
-
-
-
-
-
-
-
1
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
1
4
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-