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Literature summary for 2.5.1.36 extracted from

  • Akashi, T.; Sasaki, K.; Aoki, T.; Ayabe, S.; Yazaki, K.
    Molecular cloning and characterization of a cDNA for pterocarpan 4-dimethylallyltransferase catalyzing the key prenylation step in the biosynthesis of glyceollin, a soybean phytoalexin (2009), Plant Physiol., 149, 683-693.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.36

Cloned(Commentary)

Cloned (Comment) Organism
expressed using recombinant yeast microsomes Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.068
-
 (6aS,11aS)-3,6a,9-trihydroxypterocarpan 30°C Glycine max
0.15
-
 dimethylallyl diphosphate 30°C Glycine max
45
-
 (6aS,11aS)-3,6a,9-trihydroxypterocarpan using microsomes of cultured soybean cells Glycine max
68
-
 (6aS,11aS)-3,6a,9-trihydroxypterocarpan using recombinant yeast microsomes Glycine max
150
-
 dimethylallyl diphosphate using recombinant yeast microsomes Glycine max
180
-
 dimethylallyl diphosphate using microsomes of cultured soybean cells Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
plastid sequence contains a plastid targeting signal Glycine max 9536
-
plastid protein sequence is led by a plastid targeting signal sequence Glycine max 9536
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 50% activity Glycine max
Mg2+ 100% activity Glycine max
Mg2+ requirement for divalent cations, with Mg2+ being more effective than Mn2+ Glycine max
Mn2+ 68% activity Glycine max
Mn2+ requirement for divalent cations, with Mg2+ being more effective than Mn2+ Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max B9A1Q4
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification sequence contains a plastid targeting signal Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Glycine max
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 dimethylallyl diphosphate + 2 (6aS,11aS)-3,6a,9-trihydroxypterocarpan
-
 Glycine max 2 diphosphate + (6aS,11aS)-2-dimethylallyl-3,6a,9-trihydroxypterocarpan + (6aS,11aS)-4-dimethylallyl-3,6a,9-trihydroxypterocarpan
-
 ?
dimethylallyl diphosphate + (-)-maackiain poor substrate Glycine max diphosphate + ?
-
 ?
dimethylallyl diphosphate + (6aS,11aS)-3,6a,9-trihydroxypterocarpan
-
 Glycine max diphosphate + (6aS,11aS)-2-dimethylallyl-3,6a,9-trihydroxypterocarpan + (6aS,11aS)-4-dimethylallyl-3,6a,9-trihydroxypterocarpan
-
 ?
additional information no substrate: genistein and daidzein Glycine max ?
-
 ?

Synonyms

Synonyms Comment Organism
(6aS,11aS)-3,9,6a-trihydroxypterocarpan [(2)-glycinol] 4-dimethylallyltransferase
-
 Glycine max
G4DT
-
 Glycine max
pterocarpan 4-dimethylallyltransferase
-
 Glycine max

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay Glycine max

Expression

Organism Comment Expression
Glycine max expression is strongly up-regulated in 5 to 24 h after elicitation of phytoalexin biosynthesis in cell culture up
Glycine max expression of the G4DT gene is strongly up-regulated in 5 to 24 h after elicitation of phytoalexin biosynthesis in cultured soybean cells up