General Stability | Organism |
---|---|
addition of 2-mercaptoethanol does not affect stability | Aspergillus terreus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Aspulvinone E dimethyl ether | - |
Aspergillus terreus | |
Bromophenol blue | - |
Aspergillus terreus | |
diphosphate | noncompetitive against aspulvinone, mixed type against dimethylallyl diphosphate | Aspergillus terreus | |
isopentenyl diphosphate | - |
Aspergillus terreus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0077 | - |
aspulvinone G | - |
Aspergillus terreus | |
0.0137 | - |
aspulvinone E | - |
Aspergillus terreus | |
0.04 | - |
dimethylallyl diphosphate | - |
Aspergillus terreus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
6 * 45000, SDS-PAGE | Aspergillus terreus |
240000 | 270000 | gel filtration | Aspergillus terreus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Aspergillus terreus | enzyme is involved in biosynthesis of aspulvinone pigments | ? | - |
? | |
additional information | Aspergillus terreus IAM 2054 | enzyme is involved in biosynthesis of aspulvinone pigments | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus terreus | - |
IAM 2054 | - |
Aspergillus terreus IAM 2054 | - |
IAM 2054 | - |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus terreus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Aspergillus terreus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0294 | - |
- |
Aspergillus terreus |
Storage Stability | Organism |
---|---|
-20°C, stable for 1 week | Aspergillus terreus |
4°C, 50% loss of activity after 3 weeks | Aspergillus terreus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-3-methylpent-2-enyl diphosphate + aspulvinone E | 35% of the activity with dimethylallyl diphosphate | Aspergillus terreus | ? | - |
? | |
(E)-3-methylpent-2-enyl diphosphate + aspulvinone E | 35% of the activity with dimethylallyl diphosphate | Aspergillus terreus IAM 2054 | ? | - |
? | |
(E)-butenyl diphosphate + aspulvinone E | 10% of the activity with dimethylallyl diphosphate | Aspergillus terreus | ? | - |
? | |
cycloheptylideneethyl diphosphate + aspulvinone E | 10% of the activity with dimethylallyl diphosphate | Aspergillus terreus | ? | - |
? | |
cyclohexylideneethyl diphosphate + aspulvinone E | 35% of the activity with dimethylallyl diphosphate | Aspergillus terreus | ? | - |
? | |
cyclohexylideneethyl diphosphate + aspulvinone E | 35% of the activity with dimethylallyl diphosphate | Aspergillus terreus IAM 2054 | ? | - |
? | |
cyclopentylideneethyl diphosphate + aspulvinone E | 35% of the activity with dimethylallyl diphosphate | Aspergillus terreus | ? | - |
? | |
cyclopentylideneethyl diphosphate + aspulvinone E | 35% of the activity with dimethylallyl diphosphate | Aspergillus terreus IAM 2054 | ? | - |
? | |
dimethylallyl diphosphate + aspulvinone E | - |
Aspergillus terreus | diphosphate + aspulvinone H | - |
? | |
dimethylallyl diphosphate + aspulvinone E | - |
Aspergillus terreus IAM 2054 | diphosphate + aspulvinone H | - |
? | |
dimethylallyl diphosphate + aspulvinone G | - |
Aspergillus terreus | ? | - |
? | |
dimethylallyl diphosphate + aspulvinone I | 75% of the activity with aspulvinone E | Aspergillus terreus | ? | - |
? | |
dimethylallyl diphosphate + aspulvinone I-c | 13% of the activity with aspulvinone E | Aspergillus terreus | ? | - |
? | |
dimethylallyl diphosphate + atromentic acid | 7% of the activity with aspulvinone E | Aspergillus terreus | ? | - |
? | |
dimethylallyl diphosphate + pulvinone | 6% of the activity with aspulvinone E | Aspergillus terreus | ? | - |
? | |
additional information | enzyme is involved in biosynthesis of aspulvinone pigments | Aspergillus terreus | ? | - |
? | |
additional information | enzyme is involved in biosynthesis of aspulvinone pigments | Aspergillus terreus IAM 2054 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 45000, SDS-PAGE | Aspergillus terreus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Aspergillus terreus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0178 | - |
diphosphate | inhibition against dimethylallyl diphosphate | Aspergillus terreus | |
0.05 | - |
diphosphate | inhibition against aspulvinone | Aspergillus terreus |