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Literature summary for 2.5.1.34 extracted from

  • Fan, A.; Xie, X.; Li, S.M.
    Tryptophan prenyltransferases showing higher catalytic activities for Friedel-Crafts alkylation of o- and m-tyrosines than tyrosine prenyltransferases (2015), Org. Biomol. Chem., 13, 7551-7557.
    View publication on PubMed
Search for more literature for 2.5.1.34

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.47
-
 L-o-tyrosine pH 7.5, 37°C, recombinant enzyme Aspergillus fumigatus
0.9
-
 L-m-tyrosine pH 7.5, 37°C, recombinant enzyme Aspergillus fumigatus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dimethylallyl diphosphate + L-tryptophan Aspergillus fumigatus C-4 prenylation diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus Q50EL0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + L-m-tyrosine C-4 and C-6 prenylation, proposed reaction mechanism, overview Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-L-m-tyrosine + 6-(3-methylbut-2-enyl)-L-m-tyrosine
-
 ?
dimethylallyl diphosphate + L-o-tyrosine C-5 prenylation, proposed reaction mechanism, overview Aspergillus fumigatus diphosphate + 5-(3-methylbut-2-enyl)-L-o-tyrosine
-
 ?
dimethylallyl diphosphate + L-tryptophan C-4 prenylation Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan
-
 ?
dimethylallyl diphosphate + L-tyrosine C-3 prenylation Aspergillus fumigatus diphosphate + 3-(3-methylbut-2-enyl)-L-tyrosine
-
 ?
additional information HR-ESI-MS and NMR product analysis Aspergillus fumigatus ?
-
 ?

Synonyms

Synonyms Comment Organism
FgaPT2
-
 Aspergillus fumigatus
tryptophan C4-prenyltransferase
-
 Aspergillus fumigatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Aspergillus fumigatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0065
-
 L-m-tyrosine pH 7.5, 37°C, recombinant enzyme Aspergillus fumigatus
0.29
-
 L-o-tyrosine pH 7.5, 37°C, recombinant enzyme Aspergillus fumigatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Aspergillus fumigatus

General Information

General Information Comment Organism
evolution the enzyme is part of the DMATS superfamily Aspergillus fumigatus
metabolism the enzyme is involved in the biosynthesis of fumigaclavine C Aspergillus fumigatus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0072
-
 L-m-tyrosine pH 7.5, 37°C, recombinant enzyme Aspergillus fumigatus
0.617
-
 L-o-tyrosine pH 7.5, 37°C, recombinant enzyme Aspergillus fumigatus