Literature summary for 2.5.1.34 extracted from

Metzger, U.; Schall, C.; Zocher, G.; Unsoeld, I.; Stec, E.; Li, S.M.; Heide, L.; Stehle, T.
The structure of dimethylallyl tryptophan synthase reveals a common architecture of aromatic prenyltransferases in fungi and bacteria (2009), Proc. Natl. Acad. Sci. USA, 106, 14309-14314.

Cloned(Commentary)

Cloned (Comment)	Organism
expressed as a His-tagged fusion protein	Aspergillus fumigatus

Crystallization (Commentary)

Crystallization (Comment)	Organism
x-ray structure of DMATS, determined at a resolution of 1.76 A is reported. A complex of DMATS with its substrate L-tryptophan and with an analogue of its isoprenoid substrate dimethylallyl diphosphate reveals the structural basis of this enzyme-catalyzed Friedel-Crafts reaction, showing strict regiospecificity for position 4 of the indole nucleus of tryptophan as well as unusual independence of the presence of Mg2+ ions. The 3D structure of DMATS belongs to a rare alpha/beta barrel fold, called prenyltransferase barrel, that is present in a small group of bacterial enzymes with no sequence similarity to DMATS	Aspergillus fumigatus

Crystallization (Comment)

Organism

x-ray structure of DMATS, determined at a resolution of 1.76 A is reported. A complex of DMATS with its substrate L-tryptophan and with an analogue of its isoprenoid substrate dimethylallyl diphosphate reveals the structural basis of this enzyme-catalyzed Friedel-Crafts reaction, showing strict regiospecificity for position 4 of the indole nucleus of tryptophan as well as unusual independence of the presence of Mg2+ ions. The 3D structure of DMATS belongs to a rare alpha/beta barrel fold, called prenyltransferase barrel, that is present in a small group of bacterial enzymes with no sequence similarity to DMATS

Aspergillus fumigatus

Organism

Organism	UniProt	Comment	Textmining
Aspergillus fumigatus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
using Ni-NTA chromatography	Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dimethylallyl S-thiolodiphosphate + L-tryptophan	dimethylallyl S-thiolodiphosphate serves as an analogue to the natural substrate, dimethylallyl diphosphate	Aspergillus fumigatus	?	-	?

Synonyms

Synonyms	Comment	Organism
dimethylallyltryptophan synthase	-	Aspergillus fumigatus
DMATS	-	Aspergillus fumigatus

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Release 2023.1 (January 2023)