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Literature summary for extracted from

  • Luk, L.Y.; Tanner, M.E.
    Mechanism of dimethylallyltryptophan synthase: evidence for a dimethylallyl cation intermediate in an aromatic prenyltransferase reaction (2009), J. Am. Chem. Soc., 131, 13932-13933.
    View publication on PubMed


Cloned (Comment) Organism
overexpressed in Escherichia coli as a C-terminal His-tagged fusion protein Aspergillus fumigatus


Inhibitors Comment Organism Structure
EDTA 3 mM EDTA leads to a 2fold reduction Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
dimethylallyl diphosphate
Aspergillus fumigatus
Aspergillus fumigatus


Metals/Ions Comment Organism Structure
additional information the enzyme does not require exogenous divalent metal ions for activity Aspergillus fumigatus


Organism UniProt Comment Textmining
Aspergillus fumigatus


Reaction Comment Organism Reaction ID
dimethylallyl diphosphate + L-tryptophan = diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan studies are presented that strongly support the dissociative mechanism for the DMAT synthase reaction. The observation of positional isotope exchange (PIX) in isotopically labeled DMAPP provides evidence for the existence of a dimethylallyl carbocation intermediate Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + L-tryptophan
Aspergillus fumigatus diphosphate + 4-dimethylallyl-L-tryptophan


Synonyms Comment Organism
dimethylallyltryptophan synthase
Aspergillus fumigatus
DMAT synthase
Aspergillus fumigatus