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Literature summary for 2.5.1.34 extracted from

  • Kremer, A.; Li, S.M.
    Potential of a 7-dimethylallyltryptophan synthase as a tool for production of prenylated indole derivatives (2008), Appl. Microbiol. Biotechnol., 79, 951-961.
    View publication on PubMed
Search for more literature for 2.5.1.34

Cloned(Commentary)

Cloned (Comment) Organism
into pGEM-T and subsequently into pQE60 for expression in Escherichia coli XL1 Blue MRF cells Aspergillus fumigatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 determined by SDS-PAGE Aspergillus fumigatus
54000
-
 calculated for His6-7-DMATS Aspergillus fumigatus
65000
-
 determined by gel filtration Aspergillus fumigatus

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
-
 isoform FgaPT2
-
Aspergillus fumigatus Q50EL0
-
-

Purification (Commentary)

Purification (Comment) Organism
His6-7-DMATS is purified with Ni-NTA agarose Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + 1-methyl-L-tryptophan
-
 Aspergillus fumigatus diphosphate + 1-methyl-4-(3-methylbut-2-enyl)-L-tryptophan 0.4% of the yield with L-tryptophan ?
dimethylallyl diphosphate + 11-methyl-DL-tryptophan
-
 Aspergillus fumigatus diphosphate + 11-methyl-DL-4-(gamma,gamma-dimethylallyl)tryptophan 1.6% of the yield with L-tryptophan ?
dimethylallyl diphosphate + 11-methyl-DL-tryptophan
-
 Aspergillus fumigatus ?
-
 ?
dimethylallyl diphosphate + 4-methyl-DL-tryptophan
-
 Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-DL-tryptophan + ? 0.2% of the yield with L-tryptophan ?
dimethylallyl diphosphate + 5-bromo-DL-tryptophan
-
 Aspergillus fumigatus diphosphate + 5-bromo-4-(3-methylbut-2-enyl)-DL-tryptophan 0.2% of the yield with L-tryptophan ?
dimethylallyl diphosphate + 5-fluoro-L-tryptophan
-
 Aspergillus fumigatus diphosphate + 5-fluoro-4-(3-methylbut-2-enyl)-L-tryptophan 0.2% of the yield with L-tryptophan ?
dimethylallyl diphosphate + 5-hydroxy-L-tryptophan
-
 Aspergillus fumigatus diphosphate + 5-hydroxy-4-(3-methylbut-2-enyl)-L-tryptophan 21.0% of the yield with L-tryptophan ?
dimethylallyl diphosphate + 5-methoxy-DL-tryptophan
-
 Aspergillus fumigatus diphosphate + 5-methoxy-4-(3-methylbut-2-enyl)-DL-tryptophan 0.6% of the yield with L-tryptophan ?
dimethylallyl diphosphate + 5-methyl-DL-tryptophan
-
 Aspergillus fumigatus diphosphate + 5-methyl-4-(3-methylbut-2-enyl)-DL-tryptophan 8.1% of the yield with L-tryptophan ?
dimethylallyl diphosphate + 6-fluoro-DL-tryptophan
-
 Aspergillus fumigatus diphosphate + 6-fluoro-4-(3-methylbut-2-enyl)-DL-tryptophan 0.2% of the yield with L-tryptophan ?
dimethylallyl diphosphate + 6-methyl-DL-tryptophan
-
 Aspergillus fumigatus diphosphate + 6-methyl-4-(3-methylbut-2-enyl)-DL-tryptophan 15.2% of the yield with L-tryptophan ?
dimethylallyl diphosphate + 7-methyl-DL-tryptophan
-
 Aspergillus fumigatus diphosphate + 7-methyl-4-(3-methylbut-2-enyl)-DL-tryptophan + ? 16.3% of the yield with L-tryptophan ?
dimethylallyl diphosphate + D-tryptophan
-
 Aspergillus fumigatus diphosphate + D-4-(3-methylbut-2-enyl)-tryptophan 9.7% of the yield with L-tryptophan ?
dimethylallyl diphosphate + D-tryptophan
-
 Aspergillus fumigatus ?
-
 ?
dimethylallyl diphosphate + DL-indole-3-lactic acid
-
 Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-DL-indole-3-lactic acid 10.5% of the yield with L-tryptophan ?
dimethylallyl diphosphate + DL-indole-3-lactic acid
-
 Aspergillus fumigatus ?
-
 ?
dimethylallyl diphosphate + indole-3-acetic acid
-
 Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-indole-3-acetic acid 0.2% of the yield with L-tryptophan ?
dimethylallyl diphosphate + indole-3-acetic acid
-
 Aspergillus fumigatus ?
-
 ?
dimethylallyl diphosphate + indole-3-butyric acid
-
 Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-indole-3-butyric acid 0.2% of the yield with L-tryptophan ?
dimethylallyl diphosphate + indole-3-butyric acid
-
 Aspergillus fumigatus ?
-
 ?
dimethylallyl diphosphate + indole-3-propionic acid
-
 Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-indole-3-propionic acid 32.2% of the yield with L-tryptophan ?
dimethylallyl diphosphate + indole-3-propionic acid
-
 Aspergillus fumigatus ?
-
 ?
dimethylallyl diphosphate + indole-3-pyruvic acid
-
 Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-indole-3-pyruvic acid 29.4% of the yield with L-tryptophan ?
dimethylallyl diphosphate + indole-3-pyruvic acid
-
 Aspergillus fumigatus ?
-
 ?
dimethylallyl diphosphate + L-abrine
-
 Aspergillus fumigatus ?
-
 ?
dimethylallyl diphosphate + L-beta-homotryptophan
-
 Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-L-beta-homotryptophan 51.5% of the yield with L-tryptophan ?
dimethylallyl diphosphate + L-beta-homotryptophan
-
 Aspergillus fumigatus ?
-
 ?
dimethylallyl diphosphate + L-tryptophan
-
 Aspergillus fumigatus diphosphate + L-4-(3-methylbut-2-enyl)-tryptophan
-
 ?
dimethylallyl diphosphate + L-tryptophan
-
 Aspergillus fumigatus ?
-
 ?
dimethylallyl diphosphate + L-tryptophan hydroxamate
-
 Aspergillus fumigatus diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan hydroxamate 22.4% of the yield with L-tryptophan ?
dimethylallyl diphosphate + L-tryptophan hydroxamate
-
 Aspergillus fumigatus ?
-
 ?
dimethylallyl diphosphate + L-tryptophan methyl ester
-
 Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan methyl ester 7.2% of the yield with L-tryptophan ?
dimethylallyl diphosphate + L-tryptophan methyl ester
-
 Aspergillus fumigatus ?
-
 ?
dimethylallyl diphosphate + N-acetyl-DL-tryptophan
-
 Aspergillus fumigatus diphosphate + N-acetyl-DL-4-(gamma,gamma-dimethylallyl)tryptophan 0.2% of the yield with L-tryptophan ?
dimethylallyl diphosphate + N-acetyl-DL-tryptophan
-
 Aspergillus fumigatus ?
-
 ?
dimethylallyl diphosphate + Nalpha-methyl-L-tryptophan i.e. L-abrine Aspergillus fumigatus diphosphate + Nalpha-methyl-L-4-(gamma,gamma-dimethylallyl)tryptophan 58.0% of the yield with L-tryptophan ?
dimethylallyl diphosphate + tryptamin
-
 Aspergillus fumigatus ?
-
 ?
dimethylallyl diphosphate + tryptamine
-
 Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-tryptamine 4.6% of the yield with L-tryptophan ?

Subunits

Subunits Comment Organism
monomer
-
 Aspergillus fumigatus

Synonyms

Synonyms Comment Organism
7-dimethylallyltryptophan synthase
-
 Aspergillus fumigatus
7-DMATS
-
 Aspergillus fumigatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 enzyme assay Aspergillus fumigatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 enzyme assay Aspergillus fumigatus