Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) pLysS cells | Aspergillus fumigatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
L-tryptophan | in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 | Aspergillus fumigatus | |
0.039 | - |
5-methyl-DL-tryptophan | in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 | Aspergillus fumigatus | |
0.103 | - |
6-methyl-DL-tryptophan | in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 | Aspergillus fumigatus | |
0.129 | - |
7-methyl-DL-tryptophan | in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 | Aspergillus fumigatus | |
0.13 | - |
N-methyl-L-tryptophan | in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 | Aspergillus fumigatus | |
0.186 | - |
L-tryptophan methyl ester | in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 | Aspergillus fumigatus | |
0.242 | - |
L-beta-homotryptophan | in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 | Aspergillus fumigatus | |
0.35 | - |
alpha-methyl-DL-tryptophan | in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 | Aspergillus fumigatus | |
0.509 | - |
5-hydroxy-L-tryptophan | in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 | Aspergillus fumigatus | |
0.558 | - |
Indole-3-propionic acid | in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 | Aspergillus fumigatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
SDS-PAGE | Aspergillus fumigatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose chromatography | Aspergillus fumigatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + 5-hydroxy-L-tryptophan | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-5-hydroxy-L-tryptophan | - |
? | |
dimethylallyl diphosphate + 5-methyl-DL-tryptophan | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-5-methyl-DL-tryptophan | - |
? | |
dimethylallyl diphosphate + 6-methyl-DL-tryptophan | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-6-methyl-DL-tryptophan | - |
? | |
dimethylallyl diphosphate + alpha-methyl-DL-tryptophan | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-alpha-methyl-DL-tryptophan | - |
? | |
dimethylallyl diphosphate + D-tryptophan | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-D-tryptophan | - |
? | |
dimethylallyl diphosphate + DL-indole-3-lactic acid | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-DL-indole-3-lactic acid | - |
? | |
dimethylallyl diphosphate + indole-3-propionic acid | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-indole-3-propionic acid | - |
? | |
dimethylallyl diphosphate + indole-3-pyruvic acid | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-indole-3-pyruvic acid | - |
? | |
dimethylallyl diphosphate + L-beta-homotryptophan | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-L-homotryptophan | - |
? | |
dimethylallyl diphosphate + L-tryptophan | - |
Aspergillus fumigatus | diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan | - |
? | |
dimethylallyl diphosphate + L-tryptophan hydroxamate | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan hydroxamate | - |
? | |
dimethylallyl diphosphate + L-tryptophan methyl ester | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan methyl ester | - |
? | |
dimethylallyl diphosphate + N-methyl-L-tryptophan | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-N-methyl-L-tryptophan | - |
? | |
dimethylallyl diphosphate + serotonin | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-serotonin | - |
? | |
dimethylallyl diphosphate + tryptamin | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-tryptamin | - |
? | |
diphosphate + 7-methyl-DL-tryptophan | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-7-methyl-DL-tryptophan | - |
? | |
additional information | halogenated derivatives of tryptophan are not accepted as substrates | Aspergillus fumigatus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
4-dimethylallyltryptophan synthase | - |
Aspergillus fumigatus |
FgaPT2 | - |
Aspergillus fumigatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0533 | - |
6-methyl-DL-tryptophan | in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 | Aspergillus fumigatus | |
0.0683 | - |
5-methyl-DL-tryptophan | in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 | Aspergillus fumigatus | |
0.1033 | - |
alpha-methyl-DL-tryptophan | in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 | Aspergillus fumigatus | |
0.1267 | - |
7-methyl-DL-tryptophan | in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 | Aspergillus fumigatus | |
0.1367 | - |
L-tryptophan methyl ester | in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 | Aspergillus fumigatus | |
0.165 | - |
5-hydroxy-L-tryptophan | in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 | Aspergillus fumigatus | |
0.252 | - |
N-methyl-L-tryptophan | in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 | Aspergillus fumigatus | |
0.2667 | - |
L-tryptophan | in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 | Aspergillus fumigatus | |
0.2683 | - |
Indole-3-propionic acid | in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 | Aspergillus fumigatus | |
0.3767 | - |
L-beta-homotryptophan | in 50 mM Tris/HCl (pH 7.5) with 5 mM CaCl2 | Aspergillus fumigatus |