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Literature summary for 2.5.1.31 extracted from

  • Danley, D.E.; Baima, E.T.; Mansour, M.; Fennell, K.F.; Chrunyk, B.A.; Mueller, J.P.; Liu, S.; Qiu, X.
    Discovery and structural characterization of an allosteric inhibitor of bacterial cis-prenyltransferase (2015), Protein Sci., 24, 20-26.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.31

Cloned(Commentary)

Cloned (Comment) Organism
gene uppS, recombinant His-tagged enzyme expression in Escherichia coli strain BL21 Streptococcus pneumoniae

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme free or in complex with substrate (2E,6E)-farnesyl diphosphate, X-ray diffraction structure determination and analysis at resolutions of 2.2 A and 2.1A, respectively Streptococcus pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
3-(2-chlorophenyl)-N-(4-isopropylphenyl-5-methylisoxazole-4-carboxamide) i.e. UK-106051, a drug-like UPPs inhibitor, UK-106051 binds to a site in the middle of this binding cleft. Substrates FPP and IPP cannot bind simultaneously to UPPs-inhibitor binary complexes Streptococcus pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate Streptococcus pneumoniae
-
 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae Q8DRB3
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and gel filtration Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
 Streptococcus pneumoniae 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
undecaprenyl pyrophosphate synthase
-
 Streptococcus pneumoniae
UPPs
-
 Streptococcus pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Streptococcus pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Streptococcus pneumoniae

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00005
-
 pH 7.5, 37°C Streptococcus pneumoniae 3-(2-chlorophenyl)-N-(4-isopropylphenyl-5-methylisoxazole-4-carboxamide)

General Information

General Information Comment Organism
physiological function the enzyme is an essential enzyme in a key bacterial cell wall synthesis pathway. It catalyzes the consecutive condensations of isopentenyl diphosphate groups on to a trans-farnesyl diphosphate to produce the C55 isoprenoid undecaprenyl diphosphate Streptococcus pneumoniae