Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.31 extracted from

  • Khare, Y.; Zhang, Y.W.; Fujihashi, M.; Miki, K.; Koyama, T.
    Significance of highly conserved aromatic residues in Micrococcus luteus B-P 26 undecaprenyl diphosphate synthase (2003), J. Biochem., 134, 819-826.
    View publication on PubMed
Search for more literature for 2.5.1.31

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of wild-type and mutant enzymes in Escherichia coli strain JM109 Micrococcus luteus

Protein Variants

Protein Variants Comment Organism
F207S site-directed mutagenesis, reduced activity, and 13fold increased Km for isopentenyl diphosphate compared to the wild-type enzyme Micrococcus luteus
F227S site-directed mutagenesis Micrococcus luteus
W210A site-directed mutagenesis, reduced activity, unaltered Km for both substrates compared to the wild-type enzyme Micrococcus luteus
W224A site-directed mutagenesis, reduced activity, 6fold increased Km for farnesyl diphosphate, and 14fold increased Km for isopentenyl diphosphate compared to the wild-type enzyme Micrococcus luteus
Y148F site-directed mutagenesis, reduced activity, unaltered Km for both substrates compared to the wild-type enzyme Micrococcus luteus
Y148S site-directed mutagenesis, inactive mutant Micrococcus luteus
Y71S site-directed mutagenesis, reduced activity, unaltered Km for both substrates compared to the wild-type enzyme Micrococcus luteus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0015
-
 farnesyl diphosphate pH 7.5, 30°C, recombinant mutant W210A Micrococcus luteus
0.0015
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 30°C, W210A mutant Micrococcus luteus
0.0028
-
 farnesyl diphosphate pH 7.5, 30°C, recombinant mutant Y148F Micrococcus luteus
0.0028
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 30°C, Y148F mutant Micrococcus luteus
0.0077
-
 farnesyl diphosphate pH 7.5, 30°C, recombinant mutant Y71S Micrococcus luteus
0.0077
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 30°C, Y71S mutant Micrococcus luteus
0.0078
-
 isopentenyl diphosphate pH 7.5, 30°C, recombinant wild-type enzyme Micrococcus luteus
0.0078
-
 isopentenyl diphosphate pH 7.5, 30°C, wild type Micrococcus luteus
0.0083
-
 farnesyl diphosphate pH 7.5, 30°C, recombinant wild-type enzyme Micrococcus luteus
0.0083
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 30°C, wild type Micrococcus luteus
0.01213
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 30°C, W224A mutant Micrococcus luteus
0.0175
-
 isopentenyl diphosphate pH 7.5, 30°C, W210A mutant Micrococcus luteus
0.018
-
 isopentenyl diphosphate pH 7.5, 30°C, recombinant mutant W210A Micrococcus luteus
0.022
-
 farnesyl diphosphate pH 7.5, 30°C, recombinant mutant F207S Micrococcus luteus
0.0222
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 30°C, F207S mutant Micrococcus luteus
0.0229
-
 isopentenyl diphosphate pH 7.5, 30°C, Y148F mutant Micrococcus luteus
0.023
-
 isopentenyl diphosphate pH 7.5, 30°C, recombinant mutant Y148F Micrococcus luteus
0.0254
-
 isopentenyl diphosphate pH 7.5, 30°C, Y71S mutant Micrococcus luteus
0.026
-
 isopentenyl diphosphate pH 7.5, 30°C, recombinant mutant Y71S Micrococcus luteus
0.0356
-
 isopentenyl diphosphate pH 7.5, 30°C, W224A mutant Micrococcus luteus
0.036
-
 isopentenyl diphosphate pH 7.5, 30°C, recombinant mutant Y148F Micrococcus luteus
0.103
-
 isopentenyl diphosphate pH 7.5, 30°C, recombinant mutant F207S Micrococcus luteus
0.1031
-
 isopentenyl diphosphate pH 7.5, 30°C, F207S mutant Micrococcus luteus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Micrococcus luteus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate Micrococcus luteus catalyzes the sequential condensation of eight molecules of isopentenyl diphosphate (IPP) in the cis-configuration into farnesyl diphosphate (FPP) to produce undecaprenyl diphosphate (UPP), which is indispensable for the biosynthesis of the bacterial cell wall 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate Micrococcus luteus B-P 26 catalyzes the sequential condensation of eight molecules of isopentenyl diphosphate (IPP) in the cis-configuration into farnesyl diphosphate (FPP) to produce undecaprenyl diphosphate (UPP), which is indispensable for the biosynthesis of the bacterial cell wall 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
 ?
farnesyl diphosphate + isopentenyl diphosphate Micrococcus luteus sequential condensation of 8 molecules of isopentenyl diphosphate in the cis-configuration into farnesyl diphosphate to produce undecaprenyl diphosphate, indispensable for the biosynthesis of bacterial cell wall diphosphate + di-trans-poly-cis-undecaprenyl diphosphate
-
 ?
farnesyl diphosphate + isopentenyl diphosphate Micrococcus luteus B-P 26 sequential condensation of 8 molecules of isopentenyl diphosphate in the cis-configuration into farnesyl diphosphate to produce undecaprenyl diphosphate, indispensable for the biosynthesis of bacterial cell wall diphosphate + di-trans-poly-cis-undecaprenyl diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Micrococcus luteus
-
-
-
Micrococcus luteus O82827 B-P 26
-
Micrococcus luteus B-P 26
-
-
-
Micrococcus luteus B-P 26 O82827 B-P 26
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Micrococcus luteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
 Micrococcus luteus 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate catalyzes the sequential condensation of eight molecules of isopentenyl diphosphate (IPP) in the cis-configuration into farnesyl diphosphate (FPP) to produce undecaprenyl diphosphate (UPP), which is indispensable for the biosynthesis of the bacterial cell wall Micrococcus luteus 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
 Micrococcus luteus B-P 26 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate catalyzes the sequential condensation of eight molecules of isopentenyl diphosphate (IPP) in the cis-configuration into farnesyl diphosphate (FPP) to produce undecaprenyl diphosphate (UPP), which is indispensable for the biosynthesis of the bacterial cell wall Micrococcus luteus B-P 26 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
 ?
di-trans-poly-cis-decaprenyl diphosphate + isopentenyl diphosphate di-trans-poly-cis-decaprenyl diphosphate is farnesyl diphosphate, isopentenyl diphosphate binding mode determination Micrococcus luteus diphosphate + di-trans-poly-cis-undecaprenyl diphosphate
-
 ?
di-trans-poly-cis-decaprenyl diphosphate + isopentenyl diphosphate di-trans-poly-cis-decaprenyl diphosphate is farnesyl diphosphate, isopentenyl diphosphate binding mode determination Micrococcus luteus B-P 26 diphosphate + di-trans-poly-cis-undecaprenyl diphosphate
-
 ?
farnesyl diphosphate + isopentenyl diphosphate sequential condensation of 8 molecules of isopentenyl diphosphate in the cis-configuration into farnesyl diphosphate to produce undecaprenyl diphosphate, indispensable for the biosynthesis of bacterial cell wall Micrococcus luteus diphosphate + di-trans-poly-cis-undecaprenyl diphosphate
-
 ?
farnesyl diphosphate + isopentenyl diphosphate sequential condensation of 8 molecules of isopentenyl diphosphate in the cis-configuration into farnesyl diphosphate to produce undecaprenyl diphosphate, indispensable for the biosynthesis of bacterial cell wall Micrococcus luteus B-P 26 diphosphate + di-trans-poly-cis-undecaprenyl diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
More cis-type prenyl transferase Micrococcus luteus
undecaprenyl diphosphate synthase
-
 Micrococcus luteus
UPP synthase
-
 Micrococcus luteus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Micrococcus luteus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information turnover number for recombinant wild-type enzyme and mutant enzymes Y71S, Y148F, F207S, W210A, W224A Micrococcus luteus
0.002
-
 isopentenyl diphosphate pH 7.5, 30°C, recombinant mutant W210A Micrococcus luteus
0.002
-
 farnesyl diphosphate pH 7.5, 30°C, recombinant mutant W210A Micrococcus luteus
0.003
-
 farnesyl diphosphate pH 7.5, 30°C, recombinant mutant F207S Micrococcus luteus
0.01
-
 isopentenyl diphosphate pH 7.5, 30°C, recombinant mutant F207S Micrococcus luteus
0.015
-
 farnesyl diphosphate pH 7.5, 30°C, recombinant mutant Y148F Micrococcus luteus
0.071
-
 isopentenyl diphosphate pH 7.5, 30°C, recombinant mutant Y148F Micrococcus luteus
0.071
-
 farnesyl diphosphate pH 7.5, 30°C, recombinant mutant Y148F Micrococcus luteus
0.11
-
 isopentenyl diphosphate pH 7.5, 30°C, recombinant mutant Y71S Micrococcus luteus
0.11
-
 farnesyl diphosphate pH 7.5, 30°C, recombinant mutant Y71S Micrococcus luteus
0.115
-
 isopentenyl diphosphate pH 7.5, 30°C, recombinant mutant Y148F Micrococcus luteus
1.22
-
 farnesyl diphosphate pH 7.5, 30°C, recombinant wild-type enzyme Micrococcus luteus
1.55
-
 isopentenyl diphosphate pH 7.5, 30°C, recombinant wild-type enzyme Micrococcus luteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Micrococcus luteus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
 additional information kcat/KM-values for recombinant wild-type enzyme and mutant enzymes Y71S, Y148F, F207S, W210A, W224A Micrococcus luteus