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Literature summary for 2.5.1.31 extracted from

  • Chen, Y.H.; Chen, A.P.C.; Chen, C.T.; Wang, A.H.J.; Liang, P.H.
    Probing the conformational change of Escherichia coli undecaprenyl pyrophosphate synthase during catalysis using an inhibitor and tryptophan mutants (2002), J. Biol. Chem., 277, 7369-7376.
    View publication on PubMed
Search for more literature for 2.5.1.31

Cloned(Commentary)

Cloned (Comment) Organism
expression of mutant enzmyes W31F, W75F, W91F, W149F, W207F, and W221F as His-tag fusion proteins using Escherichia coli BL21 as host cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
W149F turnover-number is 68% of that of the wild-type enzyme, Km-value for farnesyl diphosphate is 1.75fold higher than that of the wild-type enzyme, Km-value for isopentenyl diphosphate is 4.9fold higher than that of the wild-type enzyme Escherichia coli
W207F turnover-number is 60% of that of the wild-type enzyme, Km-value for farnesyl diphosphate is 8.5fold higher than that of the wild-type enzyme, Km-value for isopentenyl diphosphate is 5.6fold higher than that of the wild-type enzyme Escherichia coli
W221F turnover-number is 140% of that of the wild-type enzyme, Km-value for farnesyl diphosphate is 1.3fold higher than that of the wild-type enzyme, Km-value for isopentenyl diphosphate is 3.4fold higher than that of the wild-type enzyme Escherichia coli
W31F turnover-number is 44% of that of the wild-type enzyme,Km-value for farnesyl diphosphate is 5fold higher than that of the wild-type enzyme, Km-value for isopentenyl diphosphate is 1.4fold higher than that of the wild-type enzyme Escherichia coli
W75F turnover-number is 44% of that of the wild-type enzyme, Km-value for farnesyl diphosphate is 4.5fold higher than that of the wild-type enzyme, Km-value for isopentenyl diphosphate is 6.3fold higher than that of the wild-type enzyme Escherichia coli
W91F turnover-number is equal to that of the wild-type enzyme, Km-value for farnesyl diphosphate is equal to that of the wild-type enzyme, Km-value for isopentenyl diphosphate is 1.8fold higher than that of the wild-type enzyme Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
(2E,6E)-farnesyl thiodiphosphate (2E,6E)-farnesyl diphosphate analogue Escherichia coli
S-farnesyl thiodiphosphate
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
 farnesyl diphosphate wild-type enzyme, pH 7.5, 25°C Escherichia coli
0.0004
-
 farnesyl diphosphate mutant enzyme W91F, pH 7.5, 25°C Escherichia coli
0.0004
-
 (2E,6E)-farnesyl diphosphate mutant enzyme W91F, pH 7.5, 25°C, wild-type enzyme, pH 7.5, 25°C Escherichia coli
0.0005
-
 (2E,6E)-farnesyl diphosphate mutant enzyme W221F, pH 7.5, 25°C Escherichia coli
0.0005
-
 farnesyl diphosphate mutant enzyme W221F, pH 7.5, 25°C Escherichia coli
0.0007
-
 (2E,6E)-farnesyl diphosphate mutant enzyme W149F, pH 7.5, 25°C Escherichia coli
0.0007
-
 farnesyl diphosphate mutant enzyme W149F, pH 7.5, 25°C Escherichia coli
0.0018
-
 (2E,6E)-farnesyl diphosphate mutant enzyme W75F, pH 7.5, 25°C Escherichia coli
0.0018
-
 farnesyl diphosphate mutant enzyme W75F, pH 7.5, 25°C Escherichia coli
0.002
-
 (2E,6E)-farnesyl diphosphate mutant enzyme W31F, pH 7.5, 25°C Escherichia coli
0.002
-
 farnesyl diphosphate mutant enzyme W31F, pH 7.5, 25°C Escherichia coli
0.0034
-
 (2E,6E)-farnesyl diphosphate mutant enzyme W207F, pH 7.5, 25°C Escherichia coli
0.0034
-
 farnesyl diphosphate mutant enzyme W207F, pH 7.5, 25°C Escherichia coli
0.0041
-
 isopentenyl diphosphate wild-type enzyme, pH 7.5, 25°C Escherichia coli
0.0059
-
 isopentenyl diphosphate mutant enzyme W31F, pH 7.5, 25°C Escherichia coli
0.0072
-
 isopentenyl diphosphate mutant enzyme W91F, pH 7.5, 25°C Escherichia coli
0.014
-
 isopentenyl diphosphate mutant enzyme W221F, pH 7.5, 25°C Escherichia coli
0.02
-
 isopentenyl diphosphate mutant enzyme W149F, pH 7.5, 25°C Escherichia coli
0.023
-
 isopentenyl diphosphate mutant enzyme W207F, pH 7.5, 25°C Escherichia coli
0.026
-
 isopentenyl diphosphate mutant enzyme W75F, pH 7.5, 25°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
 Escherichia coli 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
 ?
(2E,6E)-farnesyl thiodiphosphate + isopentenyl diphosphate weak activity, 10000000fold lower than with (2E,6E)-farnesyl diphosphate Escherichia coli ?
-
 ?
isopentenyl diphosphate + farnesyl diphosphate
-
 Escherichia coli C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
isopentenyl diphosphate + S-farnesyl thiodiphosphate weak activity, 10000000fold lower than with farnesyl diphosphate Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
undecaprenyl pyrophosphate synthase
-
 Escherichia coli
UPPs
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Escherichia coli
0.00000031
-
 (2E,6E)-farnesyl thiodiphosphate much smaller than using (2E,6E)-farnesyl diphosphate, pH 7.5, 25°C Escherichia coli
1.1
-
 (2E,6E)-farnesyl diphosphate mutant enzyme W31F, pH 7.5, 25°C Escherichia coli
1.1
-
 (2E,6E)-farnesyl diphosphate mutant enzyme W75F, pH 7.5, 25°C Escherichia coli
1.5
-
 (2E,6E)-farnesyl diphosphate mutant enzyme W207F, pH 7.5, 25°C Escherichia coli
1.7
-
 (2E,6E)-farnesyl diphosphate mutant enzyme W149F, pH 7.5, 25°C Escherichia coli
2.5
-
 (2E,6E)-farnesyl diphosphate wild-type enzyme, pH 7.5, 25°C Escherichia coli
2.5
-
 (2E,6E)-farnesyl diphosphate mutant enzyme W91F, pH 7.5, 25°C Escherichia coli
3.5
-
 (2E,6E)-farnesyl diphosphate mutant enzyme W221F, pH 7.5, 25°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0002
-
 (S)-farnesyl thiopyrophosphate
-
 Escherichia coli
0.0002
-
 (2E,6E)-farnesyl thiodiphosphate pH 7.5, 25°C Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
347
-
 (2E,6E)-farnesyl diphosphate mutant enzyme W91F, pH 7.5, 25°C Escherichia coli
441
-
 (2E,6E)-farnesyl diphosphate mutant enzyme W207F, pH 7.5, 25°C Escherichia coli
550
-
 (2E,6E)-farnesyl diphosphate mutant enzyme W31F, pH 7.5, 25°C Escherichia coli
611
-
 (2E,6E)-farnesyl diphosphate mutant enzyme W75F, pH 7.5, 25°C Escherichia coli
2428
-
 (2E,6E)-farnesyl diphosphate mutant enzyme W149F, pH 7.5, 25°C Escherichia coli
6250
-
 (2E,6E)-farnesyl diphosphate wild-type enzyme, pH 7.5, 25°C Escherichia coli
7000
-
 (2E,6E)-farnesyl diphosphate mutant enzyme W221F, pH 7.5, 25°C Escherichia coli