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Literature summary for 2.5.1.31 extracted from

  • Fujikura, K.; Zhang, Y.W.; Fujihashi, M.; Miki, K.; Koyama, T.
    Mutational analysis of allylic substrate binding site of Micrococcus luteus B-P 26 undecaprenyl diphosphate synthase (2003), Biochemistry, 42, 4035-4041.
    View publication on PubMed
Search for more literature for 2.5.1.31

Cloned(Commentary)

Cloned (Comment) Organism
mutated enzymes are overproduced in Escherichia coli Micrococcus luteus

Protein Variants

Protein Variants Comment Organism
D29A Km-value for farnesyl diphosphate is 9.2fold higher than that for the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 1.6fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 5.8fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 1% of that of the wild-type enzyme Micrococcus luteus
D29A Km-value for farnesyl diphosphate is 9.2fold higher than that for the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 1.6fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 5.8fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 1% of that of the wild-type enzyme Micrococcus luteus
E76Q Km-value for farnesyl diphosphate is 1.6fold higher than that for the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 3.6fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 1.5fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 30% of that of the wild-type enzyme Micrococcus luteus
E76Q Km-value for farnesyl diphosphate is 1.6fold higher than that for the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 3.6fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 1.5fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 30% of that of the wild-type enzyme Micrococcus luteus
E84Q Km-value for farnesyl diphosphate is 3.2fold higher than that for the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 1.3fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 3.98fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 65% of that of the wild-type enzyme Micrococcus luteus
E84Q Km-value for farnesyl diphosphate is 3.2fold higher than that for the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 1.3fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 3.98fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 65% of that of the wild-type enzyme Micrococcus luteus
G32R Km-value for farnesyl diphosphate is comparable to that of the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 1.9fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is comparable to that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 27% of that of the wild-type enzyme Micrococcus luteus
G32R Km-value for farnesyl diphosphate is comparable to that of the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 1.9fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is comparable to that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 27% of that of the wild-type enzyme Micrococcus luteus
G32R/R42G Km-value for farnesyl diphosphate is 1.4fold higher than that for the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 1.4fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 1.3fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 58% of that of the wild-type enzyme Micrococcus luteus
G32R/R42G Km-value for farnesyl diphosphate is 1.4fold higher than that for the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 1.4fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 1.3fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 58% of that of the wild-type enzyme Micrococcus luteus
R33A Km-value for farnesyl diphosphate is 40fold higher than that for the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 1.2fold lower than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 3% of that of the wild-type enzyme. Different product distribution pattern compared to the wildf-type enzyme Micrococcus luteus
R33A Km-value for farnesyl diphosphate is 40fold higher than that for the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 1.2fold lower than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 3% of that of the wild-type enzyme. Different product distribution pattern compared to the wildf-type enzyme Micrococcus luteus
R42G Km-value for farnesyl diphosphate is 1.4fold higher than that for the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 1.8fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 3.1fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 13% of that of the wild-type enzyme Micrococcus luteus
R42G Km-value for farnesyl diphosphate is 1.4fold higher than that for the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 1.8fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 3.1fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 13% of that of the wild-type enzyme Micrococcus luteus
R80A Km-value for farnesyl diphosphate is 6.4fold higher than that for the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 1.3fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 6.3fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is less than 1% of that of the wild-type enzyme Micrococcus luteus
R80A Km-value for farnesyl diphosphate is 6.4fold higher than that for the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 1.3fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 6.3fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is less than 1% of that of the wild-type enzyme Micrococcus luteus

Inhibitors

Inhibitors Comment Organism Structure
SO42- competitive inhibitor with respect to (2E,6E)-farnesyl diphosphate; competitive inhibitor with respect to farnesyl diphosphate Micrococcus luteus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0023
-
 farnesyl diphosphate mutant enzyme E76Q, pH 7.5, 37°C Micrococcus luteus
0.0023
-
 (Z,E,E)-Geranylgeranyl diphosphate mutant enzyme E76Q, pH 7.5, 37°C Micrococcus luteus
0.0023
-
 (2E,6E)-farnesyl diphosphate E76Q mutant, pH 7.5, 37°C Micrococcus luteus
0.0033
-
 farnesyl diphosphate mutant enzyme G32R, pH 7.5, 37°C Micrococcus luteus
0.0033
-
 (2E,6E)-farnesyl diphosphate G32R mutant, pH 7.5, 37°C Micrococcus luteus
0.0036
-
 farnesyl diphosphate wild-type enzyme, pH 7.5, 37°C Micrococcus luteus
0.0036
-
 (2E,6E)-farnesyl diphosphate wild-type, pH 7.5, 37°C Micrococcus luteus
0.0043
-
 (Z,E,E)-Geranylgeranyl diphosphate mutant enzyme G32R, pH 7.5, 37°C Micrococcus luteus
0.0043
-
 (2Z,6E,10E)-geranylgeranyl diphosphate G32R mutant, pH 7.5, 37°C Micrococcus luteus
0.0045
-
 (Z,E,E)-Geranylgeranyl diphosphate mutant enzyme R42G, pH 7.5, 37°C Micrococcus luteus
0.0045
-
 (2Z,6E,10E)-geranylgeranyl diphosphate R42G mutant, pH 7.5, 37°C Micrococcus luteus
0.0049
-
 farnesyl diphosphate mutant enzyme R42G, pH 7.5, 37°C Micrococcus luteus
0.0049
-
 (2E,6E)-farnesyl diphosphate R42G mutant, pH 7.5, 37°C Micrococcus luteus
0.005
-
 farnesyl diphosphate mutant enzyme G32R/R42G, pH 7.5, 37°C Micrococcus luteus
0.005
-
 (Z,E,E)-Geranylgeranyl diphosphate mutant enzyme D29A, pH 7.5, 37°C Micrococcus luteus
0.005
-
 (2Z,6E,10E)-geranylgeranyl diphosphate D29A mutant, pH 7.5, 37°C Micrococcus luteus
0.005
-
 (2E,6E)-farnesyl diphosphate G32R/R42G mutant, pH 7.5, 37°C Micrococcus luteus
0.0059
-
 isopentenyl diphosphate mutant enzyme G32R/R42G, pH 7.5, 37°C Micrococcus luteus
0.0059
-
 (Z,E,E)-Geranylgeranyl diphosphate mutant enzyme G32R/R42G, pH 7.5, 37°C Micrococcus luteus
0.0059
-
 (2Z,6E,10E)-geranylgeranyl diphosphate G32R-R42G mutant, pH 7.5, 37°C Micrococcus luteus
0.0059
-
 isopentenyl diphosphate G32R/R42G mutant, pH 7.5, 37°C Micrococcus luteus
0.006
-
 isopentenyl diphosphate mutant enzyme G32R, pH 7.5, 37°C Micrococcus luteus
0.006
-
 (Z,E,E)-Geranylgeranyl diphosphate mutant enzyme E84Q, pH 7.5, 37°C Micrococcus luteus
0.006
-
 (2Z,6E,10E)-geranylgeranyl diphosphate E84Q mutant, pH 7.5, 37°C Micrococcus luteus
0.006
-
 isopentenyl diphosphate G32R mutant, pH 7.5, 37°C Micrococcus luteus
0.0061
-
 (Z,E,E)-Geranylgeranyl diphosphate mutant enzyme R80A, pH 7.5, 37°C Micrococcus luteus
0.0061
-
 (2Z,6E,10E)-geranylgeranyl diphosphate R80A mutant, pH 7.5, 37°C Micrococcus luteus
0.0078
-
 isopentenyl diphosphate wild-type enzyme, pH 7.5, 37°C Micrococcus luteus
0.0078
-
 isopentenyl diphosphate wild-type, pH 7.5, 37°C Micrococcus luteus
0.0082
-
 (Z,E,E)-Geranylgeranyl diphosphate wild-type enzyme, pH 7.5, 37°C Micrococcus luteus
0.0082
-
 (2Z,6E,10E)-geranylgeranyl diphosphate wild-type, pH 7.5, 37°C Micrococcus luteus
0.0088
-
 (2E,6E,10E)-geranylgeranyl diphosphate wild-type, pH 7.5, 37°C Micrococcus luteus
0.0095
-
 (Z,E,E)-Geranylgeranyl diphosphate mutant enzyme R33A, pH 7.5, 37°C Micrococcus luteus
0.0095
-
 (2Z,6E,10E)-geranylgeranyl diphosphate R33A mutant, pH 7.5, 37°C Micrococcus luteus
0.0114
-
 isopentenyl diphosphate mutant enzyme E76Q, pH 7.5, 37°C Micrococcus luteus
0.0114
-
 farnesyl diphosphate mutant enzyme E84Q, pH 7.5, 37°C Micrococcus luteus
0.0114
-
 isopentenyl diphosphate E76Q mutant, pH 7.5, 37°C Micrococcus luteus
0.0114
-
 (2E,6E)-farnesyl diphosphate E84Q mutant, pH 7.5, 37°C Micrococcus luteus
0.023
-
 (2Z,6E,10E)-geranylgeranyl diphosphate E76Q mutant, pH 7.5, 37°C Micrococcus luteus
0.0231
-
 farnesyl diphosphate mutant enzyme R80A, pH 7.5, 37°C Micrococcus luteus
0.0231
-
 (2E,6E)-farnesyl diphosphate R80A mutant, pH 7.5, 37°C Micrococcus luteus
0.0242
-
 isopentenyl diphosphate mutant enzyme R42G, pH 7.5, 37°C Micrococcus luteus
0.0242
-
 isopentenyl diphosphate R42G mutant, pH 7.5, 37°C Micrococcus luteus
0.0307
-
 isopentenyl diphosphate mutant enzyme E84Q, pH 7.5, 37°C Micrococcus luteus
0.0307
-
 isopentenyl diphosphate E84Q mutant, pH 7.5, 37°C Micrococcus luteus
0.0332
-
 farnesyl diphosphate mutant enzyme D29A, pH 7.5, 37°C Micrococcus luteus
0.0332
-
 (2E,6E)-farnesyl diphosphate D29A mutant, pH 7.5, 37°C Micrococcus luteus
0.0452
-
 isopentenyl diphosphate mutant enzyme D29A, pH 7.5, 37°C Micrococcus luteus
0.0452
-
 isopentenyl diphosphate D29A mutant, pH 7.5, 37°C Micrococcus luteus
0.0491
-
 isopentenyl diphosphate mutant enzyme R80A, pH 7.5, 37°C Micrococcus luteus
0.0491
-
 isopentenyl diphosphate R80A mutant, pH 7.5, 37°C Micrococcus luteus
0.064
-
 (2E,6E,10E)-geranylgeranyl diphosphate R33A mutant, pH 7.5, 37°C Micrococcus luteus
0.144
-
 farnesyl diphosphate mutant enzyme R33A, pH 7.5, 37°C Micrococcus luteus
0.144
-
 (2E,6E)-farnesyl diphosphate R33A mutant, pH 7.5, 37°C Micrococcus luteus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Micrococcus luteus catalyzes the sequential cis-condensation of isopentenyl diphosphate onto (2E,6E)-farnesyl diphosphate ?
-
 ?
additional information Micrococcus luteus B-P 26 catalyzes the sequential cis-condensation of isopentenyl diphosphate onto (2E,6E)-farnesyl diphosphate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Micrococcus luteus
-
 B-P 26
-
Micrococcus luteus O82827 B-P 26
-
Micrococcus luteus B-P 26
-
 B-P 26
-
Micrococcus luteus B-P 26 O82827 B-P 26
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
 Micrococcus luteus 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
 Micrococcus luteus B-P 26 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
 ?
(2E,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
-
 Micrococcus luteus ?
-
 ?
(2E,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
-
 Micrococcus luteus B-P 26 ?
-
 ?
(2Z,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
-
 Micrococcus luteus ?
-
 ?
(2Z,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
-
 Micrococcus luteus B-P 26 ?
-
 ?
isopentenyl diphosphate + farnesyl diphosphate (E,E)-farnesyl diphosphate Micrococcus luteus C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
isopentenyl diphosphate + farnesyl diphosphate (E,E)-farnesyl diphosphate Micrococcus luteus B-P 26 C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
isopentenyl diphosphate + geranylgeranyl diphosphate (Z,E,E)-geranylgeranyl diphosphate Micrococcus luteus C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
isopentenyl diphosphate + geranylgeranyl diphosphate (Z,E,E)-geranylgeranyl diphosphate Micrococcus luteus B-P 26 C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
additional information catalyzes the sequential cis-condensation of isopentenyl diphosphate onto (2E,6E)-farnesyl diphosphate Micrococcus luteus ?
-
 ?
additional information catalyzes the sequential cis-condensation of isopentenyl diphosphate onto (2E,6E)-farnesyl diphosphate Micrococcus luteus B-P 26 ?
-
 ?

Synonyms

Synonyms Comment Organism
undecaprenyl diphosphate synthase
-
 Micrococcus luteus
UPP synthase
-
 Micrococcus luteus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Micrococcus luteus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.004
-
 (2E,6E)-farnesyl diphosphate R80A mutant, pH 7.5, 37°C Micrococcus luteus
0.00579
-
 (2Z,6E,10E)-geranylgeranyl diphosphate R80A mutant, pH 7.5, 37°C Micrococcus luteus
0.0167
-
 farnesyl diphosphate mutant enzyme D29A, pH 7.5, 37°C Micrococcus luteus
0.0167
-
 (2E,6E)-farnesyl diphosphate D29A mutant, pH 7.5, 37°C Micrococcus luteus
0.0258
-
 (2Z,6E,10E)-geranylgeranyl diphosphate D29A mutant, pH 7.5, 37°C Micrococcus luteus
0.0542
-
 farnesyl diphosphate mutant enzyme R33A, pH 7.5, 37°C Micrococcus luteus
0.0542
-
 (2E,6E)-farnesyl diphosphate R33A mutant, pH 7.5, 37°C Micrococcus luteus
0.0813
-
 (2Z,6E,10E)-geranylgeranyl diphosphate R33A mutant, pH 7.5, 37°C Micrococcus luteus
0.216
-
 farnesyl diphosphate mutant enzyme R42G, pH 7.5, 37°C Micrococcus luteus
0.216
-
 (2E,6E)-farnesyl diphosphate R42G mutant, pH 7.5, 37°C Micrococcus luteus
0.382
-
 (2Z,6E,10E)-geranylgeranyl diphosphate R42G mutant, pH 7.5, 37°C Micrococcus luteus
0.462
-
 farnesyl diphosphate mutant enzyme G32R, pH 7.5, 37°C Micrococcus luteus
0.462
-
 (2E,6E)-farnesyl diphosphate G32R mutant, pH 7.5, 37°C Micrococcus luteus
0.505
-
 farnesyl diphosphate mutant enzyme E76Q, pH 7.5, 37°C Micrococcus luteus
0.505
-
 (2E,6E)-farnesyl diphosphate E76Q mutant, pH 7.5, 37°C Micrococcus luteus
0.604
-
 (2Z,6E,10E)-geranylgeranyl diphosphate E76Q mutant, pH 7.5, 37°C Micrococcus luteus
0.608
-
 (2Z,6E,10E)-geranylgeranyl diphosphate G32R mutant, pH 7.5, 37°C Micrococcus luteus
0.96
-
 farnesyl diphosphate mutant enzyme G32R/R42G, pH 7.5, 37°C Micrococcus luteus
0.96
-
 (2E,6E)-farnesyl diphosphate G32R/R42G mutant, pH 7.5, 37°C Micrococcus luteus
1.07
-
 (2Z,6E,10E)-geranylgeranyl diphosphate E84Q mutant, pH 7.5, 37°C Micrococcus luteus
1.09
-
 farnesyl diphosphate mutant enzyme E84Q, pH 7.5, 37°C Micrococcus luteus
1.09
-
 (2E,6E)-farnesyl diphosphate E84Q mutant, pH 7.5, 37°C Micrococcus luteus
1.11
-
 (2Z,6E,10E)-geranylgeranyl diphosphate G32R/R42G mutant, pH 7.5, 37°C Micrococcus luteus
1.66
-
 farnesyl diphosphate wild-type enzyme, pH 7.5, 37°C Micrococcus luteus
1.66
-
 (2E,6E)-farnesyl diphosphate wild-type, pH 7.5, 37°C Micrococcus luteus
1.77
-
 (2Z,6E,10E)-geranylgeranyl diphosphate wild-type, pH 7.5, 37°C Micrococcus luteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Micrococcus luteus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
17
-
 SO42-
-
 Micrococcus luteus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.25
-
 (2E,6E)-farnesyl diphosphate R80A mutant, pH 7.5, 37°C Micrococcus luteus
0.713
-
 (2E,6E)-farnesyl diphosphate R33A mutant, pH 7.5, 37°C Micrococcus luteus
0.777
-
 (2E,6E)-farnesyl diphosphate D29A mutant, pH 7.5, 37°C Micrococcus luteus
0.949
-
 (2Z,6E,10E)-geranylgeranyl diphosphate R80A mutant, pH 7.5, 37°C Micrococcus luteus
5.2
-
 (2Z,6E,10E)-geranylgeranyl diphosphate D29A mutant, pH 7.5, 37°C Micrococcus luteus
8.65
-
 (2Z,6E,10E)-geranylgeranyl diphosphate R33A mutant, pH 7.5, 37°C Micrococcus luteus
26.3
-
 (2Z,6E,10E)-geranylgeranyl diphosphate E76Q mutant, pH 7.5, 37°C Micrococcus luteus
77.9
-
 (2E,6E)-farnesyl diphosphate R42G mutant, pH 7.5, 37°C Micrococcus luteus
84.8
-
 (2Z,6E,10E)-geranylgeranyl diphosphate R42G mutant, pH 7.5, 37°C Micrococcus luteus
93.9
-
 (2E,6E)-farnesyl diphosphate E84Q mutant, pH 7.5, 37°C Micrococcus luteus
141
-
 (2Z,6E,10E)-geranylgeranyl diphosphate G32R mutant, pH 7.5, 37°C Micrococcus luteus
178
-
 (2Z,6E,10E)-geranylgeranyl diphosphate E84Q mutant, pH 7.5, 37°C Micrococcus luteus
184
-
 (2E,6E)-farnesyl diphosphate G32R mutant, pH 7.5, 37°C Micrococcus luteus
188
-
 (2Z,6E,10E)-geranylgeranyl diphosphate G32R/R42G mutant, pH 7.5, 37°C Micrococcus luteus
215.9
-
 (2Z,6E,10E)-geranylgeranyl diphosphate wild-type, pH 7.5, 37°C Micrococcus luteus
222
-
 (2E,6E)-farnesyl diphosphate G32R/R42G mutant, pH 7.5, 37°C Micrococcus luteus
262
-
 (2E,6E)-farnesyl diphosphate E76Q mutant, pH 7.5, 37°C Micrococcus luteus
461
-
 (2E,6E)-farnesyl diphosphate wild-type, pH 7.5, 37°C Micrococcus luteus