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Literature summary for 2.5.1.31 extracted from

  • Fujikura, K.; Zhang, Y.W.; Yoshizaki, H.; Nishino, T.; Koyama, T.
    Significance of Asn-77 and Trp-78 in the catalytic function of undecaprenyl diphosphate synthase of Micrococcus luteus B-P 26 (2000), J. Biochem., 128, 917-922.
    View publication on PubMed
Search for more literature for 2.5.1.31

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Micrococcus luteus

Protein Variants

Protein Variants Comment Organism
N77A dramatic decrease in catalytic activity, but Km-values for both allylic and homoallylic substrates are comparable to those of the wild-type Micrococcus luteus
N77D dramatic decrease in catalytic activity, but Km-values for both allylic and homoallylic substrates are comparable to those of the wild-type Micrococcus luteus
N77Q dramatic decrease in catalytic activity, but Km-values for both allylic and homoallylic substrates are comparable to those of the wild-type Micrococcus luteus
W78D moderate levels of enzymatic activity and comparable Km-values for isopentenyl diphosphate to that of the wild-type. 18.7fold increase in Km-value for farnesyl diphosphate Micrococcus luteus
W78I moderate levels of enzymatic activity and comparable Km-values for isopentenyl diphosphate to that of the wild-type. 6-fold increase in Km-value for farnesyl diphosphate, 2.3fold increase in Km-value for (Z,E,E)-geranylgeranyl diphosphate Micrococcus luteus
W78I moderate levels of enzymatic activity and comparable Km-values for isopentenyl diphosphate to that of the wild-type. 6-fold increase in Km-value for farnesyl diphosphate, 2.3fold increase in Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate Micrococcus luteus
W78R moderate levels of enzymatic activity and comparable Km-values for isopentenyl diphosphate to that of the wild-type. 14.5-fold increase in Km-value for farnesyl diphosphate, 1.8fold increase in Km-value for (Z,E,E)-geranylgeranyl diphosphate Micrococcus luteus
W78R moderate levels of enzymatic activity and comparable Km-values for isopentenyl diphosphate to that of the wild-type. 14.5-fold increase in Km-value for farnesyl diphosphate, 1.8fold increase in Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate Micrococcus luteus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 (2Z,6E,10E)-geranylgeranyl diphosphate mutant enzyme N77Q, pH 7.5, 37°C Micrococcus luteus
0.005
-
 (Z,E,E)-Geranylgeranyl diphosphate mutant enzyme N77Q, pH 7.5, 37°C Micrococcus luteus
0.0078
-
 isopentenyl diphosphate wild-type enzyme, pH 7.5, 37°C Micrococcus luteus
0.008
-
 (Z,E,E)-Geranylgeranyl diphosphate mutant enzyme N77D, pH 7.5, 37°C Micrococcus luteus
0.008
-
 (Z,E,E)-Geranylgeranyl diphosphate mutant enzyme W78D, pH 7.5, 37°C Micrococcus luteus
0.008
-
 (2Z,6E,10E)-geranylgeranyl diphosphate mutant enzyme N77D, pH 7.5, 37°C; mutant enzyme W78D, pH 7.5, 37°C Micrococcus luteus
0.0082
-
 (2Z,6E,10E)-geranylgeranyl diphosphate wild-type enzyme, pH 7.5, 37°C Micrococcus luteus
0.0082
-
 (Z,E,E)-Geranylgeranyl diphosphate wild-type enzyme, pH 7.5, 37°C Micrococcus luteus
0.0083
-
 (2E,6E)-farnesyl diphosphate wild-type, pH 7.5, 37°C Micrococcus luteus
0.0083
-
 farnesyl diphosphate wild-type, pH 7.5, 37°C Micrococcus luteus
0.009
-
 isopentenyl diphosphate mutant enzyme N77Q, pH 7.5, 37°C Micrococcus luteus
0.0102
-
 (2Z,6E,10E)-geranylgeranyl diphosphate mutant enzyme N77A, pH 7.5, 37°C Micrococcus luteus
0.0102
-
 (Z,E,E)-Geranylgeranyl diphosphate mutant enzyme N77A, pH 7.5, 37°C Micrococcus luteus
0.0104
-
 (2E,6E)-farnesyl diphosphate mutant enzyme N77Q, pH 7.5, 37°C Micrococcus luteus
0.0104
-
 farnesyl diphosphate mutant enzyme N77Q, pH 7.5, 37°C Micrococcus luteus
0.012
-
 isopentenyl diphosphate mutant enzyme N77A, pH 7.5, 37°C Micrococcus luteus
0.0131
-
 isopentenyl diphosphate mutant enzyme W78D, pH 7.5, 37°C Micrococcus luteus
0.0139
-
 (2E,6E)-farnesyl diphosphate mutant enzyme N77D, pH 7.5, 37°C Micrococcus luteus
0.0139
-
 farnesyl diphosphate mutant enzyme N77D, pH 7.5, 37°C Micrococcus luteus
0.0141
-
 isopentenyl diphosphate mutant enzyme W78I, pH 7.5, 37°C Micrococcus luteus
0.0147
-
 (2E,6E)-farnesyl diphosphate mutant enzyme N77A, pH 7.5, 37°C Micrococcus luteus
0.0147
-
 farnesyl diphosphate mutant enzyme N77A, pH 7.5, 37°C Micrococcus luteus
0.015
-
 isopentenyl diphosphate mutant enzyme N77D, pH 7.5, 37°C Micrococcus luteus
0.015
-
 (2Z,6E,10E)-geranylgeranyl diphosphate mutant enzyme W78R, pH 7.5, 37°C Micrococcus luteus
0.015
-
 (Z,E,E)-Geranylgeranyl diphosphate mutant enzyme W78R, pH 7.5, 37°C Micrococcus luteus
0.0165
-
 isopentenyl diphosphate mutant enzyme W78R, pH 7.5, 37°C Micrococcus luteus
0.019
-
 (2Z,6E,10E)-geranylgeranyl diphosphate mutant enzyme W78I, pH 7.5, 37°C Micrococcus luteus
0.019
-
 (Z,E,E)-Geranylgeranyl diphosphate mutant enzyme W78I, pH 7.5, 37°C Micrococcus luteus
0.0495
-
 (2E,6E)-farnesyl diphosphate mutant enzyme W78I, pH 7.5, 37°C Micrococcus luteus
0.0495
-
 farnesyl diphosphate mutant enzyme W78I, pH 7.5, 37°C Micrococcus luteus
0.1207
-
 (2E,6E)-farnesyl diphosphate mutant enzyme W78R, pH 7.5, 37°C Micrococcus luteus
0.1207
-
 farnesyl diphosphate mutant enzyme W78R, pH 7.5, 37°C Micrococcus luteus
0.155
-
 (2E,6E)-farnesyl diphosphate mutant enzyme N78D, pH 7.5, 37°C Micrococcus luteus
0.155
-
 farnesyl diphosphate mutant enzyme N78D, pH 7.5, 37°C Micrococcus luteus

Organism

Organism UniProt Comment Textmining
Micrococcus luteus O82827 B-P 26
-
Micrococcus luteus B-P 26 O82827 B-P 26
-

Purification (Commentary)

Purification (Comment) Organism
-
 Micrococcus luteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
 Micrococcus luteus 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
 Micrococcus luteus B-P 26 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
 ?
(2Z,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
-
 Micrococcus luteus ?
-
 ?
(2Z,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
-
 Micrococcus luteus B-P 26 ?
-
 ?
isopentenyl diphosphate + farnesyl diphosphate
-
 Micrococcus luteus C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
isopentenyl diphosphate + farnesyl diphosphate
-
 Micrococcus luteus B-P 26 C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
isopentenyl diphosphate + geranylgeranyl diphosphate
-
 Micrococcus luteus C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
isopentenyl diphosphate + geranylgeranyl diphosphate
-
 Micrococcus luteus B-P 26 C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
undecaprenyl diphosphate synthase
-
 Micrococcus luteus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00125
-
 (2E,6E)-farnesyl diphosphate mutant enzyme N77A, pH 7.5, 37°C Micrococcus luteus
0.00125
-
 farnesyl diphosphate mutant enzyme N77A, pH 7.5, 37°C Micrococcus luteus
0.00155
-
 (2E,6E)-farnesyl diphosphate mutant enzyme N77Q, pH 7.5, 37°C Micrococcus luteus
0.00155
-
 farnesyl diphosphate mutant enzyme N77Q, pH 7.5, 37°C Micrococcus luteus
0.002
-
 (2Z,6E,10E)-geranylgeranyl diphosphate mutant enzyme N77A, pH 7.5, 37°C Micrococcus luteus
0.002
-
 (Z,E,E)-Geranylgeranyl diphosphate mutant enzyme N77A, pH 7.5, 37°C Micrococcus luteus
0.00231
-
 (2Z,6E,10E)-geranylgeranyl diphosphate mutant enzyme N77Q, pH 7.5, 37°C Micrococcus luteus
0.00231
-
 (Z,E,E)-Geranylgeranyl diphosphate mutant enzyme N77Q, pH 7.5, 37°C Micrococcus luteus
0.0027
-
 isopentenyl diphosphate mutant enzyme N77Q, pH 7.5, 37°C Micrococcus luteus
0.00272
-
 (2E,6E)-farnesyl diphosphate mutant enzyme N77D, pH 7.5, 37°C Micrococcus luteus
0.00272
-
 farnesyl diphosphate mutant enzyme N77D, pH 7.5, 37°C Micrococcus luteus
0.00415
-
 (2Z,6E,10E)-geranylgeranyl diphosphate mutant enzyme N77D, pH 7.5, 37°C Micrococcus luteus
0.00415
-
 (Z,E,E)-Geranylgeranyl diphosphate mutant enzyme N77D, pH 7.5, 37°C Micrococcus luteus
0.00473
-
 isopentenyl diphosphate mutant enzyme N77D, pH 7.5, 37°C Micrococcus luteus
0.0164
-
 isopentenyl diphosphate mutant enzyme N77A, pH 7.5, 37°C Micrococcus luteus
0.151
-
 (2E,6E)-farnesyl diphosphate mutant enzyme W78D, pH 7.5, 37°C Micrococcus luteus
0.151
-
 farnesyl diphosphate mutant enzyme W78D, pH 7.5, 37°C Micrococcus luteus
0.3
-
 (2Z,6E,10E)-geranylgeranyl diphosphate mutant enzyme W78D, pH 7.5, 37°C Micrococcus luteus
0.3
-
 (Z,E,E)-Geranylgeranyl diphosphate mutant enzyme W78D, pH 7.5, 37°C Micrococcus luteus
0.397
-
 isopentenyl diphosphate mutant enzyme W78D, pH 7.5, 37°C Micrococcus luteus
0.434
-
 (2E,6E)-farnesyl diphosphate mutant enzyme N78R, pH 7.5, 37°C Micrococcus luteus
0.434
-
 farnesyl diphosphate mutant enzyme N78R, pH 7.5, 37°C Micrococcus luteus
0.456
-
 (2E,6E)-farnesyl diphosphate mutant enzyme W78I, pH 7.5, 37°C Micrococcus luteus
0.456
-
 farnesyl diphosphate mutant enzyme W78I, pH 7.5, 37°C Micrococcus luteus
0.783
-
 isopentenyl diphosphate mutant enzyme W78I, pH 7.5, 37°C Micrococcus luteus
0.803
-
 isopentenyl diphosphate mutant enzyme W78R, pH 7.5, 37°C Micrococcus luteus
0.899
-
 (2Z,6E,10E)-geranylgeranyl diphosphate mutant enzyme W78R, pH 7.5, 37°C Micrococcus luteus
0.899
-
 (Z,E,E)-Geranylgeranyl diphosphate mutant enzyme W78R, pH 7.5, 37°C Micrococcus luteus
1.22
-
 (2E,6E)-farnesyl diphosphate wild-type enzyme, pH 7.5, 37°C Micrococcus luteus
1.22
-
 farnesyl diphosphate wild-type enzyme, pH 7.5, 37°C Micrococcus luteus
1.37
-
 (2Z,6E,10E)-geranylgeranyl diphosphate mutant enzyme W78I, pH 7.5, 37°C Micrococcus luteus
1.37
-
 (Z,E,E)-Geranylgeranyl diphosphate mutant enzyme W78I, pH 7.5, 37°C Micrococcus luteus
1.77
-
 (2Z,6E,10E)-geranylgeranyl diphosphate wild-type enzyme, pH 7.5, 37°C Micrococcus luteus
1.77
-
 (Z,E,E)-Geranylgeranyl diphosphate wild-type enzyme, pH 7.5, 37°C Micrococcus luteus
1.85
-
 isopentenyl diphosphate wild-type, pH 7.5, 37°C Micrococcus luteus