Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.31 extracted from

  • Fujisaki, S.; Nishino, T.; Katasuki, H.
    Isoprenoid synthesis in Escherichia coli. Separation and partial purification of four enzymes involved in the synthesis (1986), J. Biochem., 99, 1327-1337.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 no activity in absence Escherichia coli
Triton X-100 no activity in the activation by Triton X-100 is almost saturated at a concentration of 0.02% Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0007
-
farnesyl diphosphate pH 7.5, 30°C Escherichia coli
0.029
-
isopentenyl diphosphate pH 7.5, 30°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mg2+ or Mn2+ required. Optimal concentration for Mg2+ is 1.0 mM Escherichia coli
Mg2+ or Mn2+ required. Optimal concentration 1.0 mM Escherichia coli
Mn2+ or Mg2+ required Escherichia coli
Mn2+ Mn2+ or Mg2+ required Escherichia coli
Mn2+ maximal stimulation at 0.05-0.1 mM, Mg2+ or Mn2+ required Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, activity in enzyme preparation is stable for several weeks Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
farnesyl diphosphate + 8 isopentenyl diphosphate neither dimethylallyl diphosphate nor geranyl diphosphate act as priming substrate for the enzyme Escherichia coli 8 diphosphate + undecaprenyl diphosphate
-
?
isopentenyl diphosphate + farnesyl diphosphate
-
Escherichia coli C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate with 0.02% Triton X-100 the main product is the C55-compound, C50-compound is the minor product. With 0.1% Triton X-100 the C50-polyprenyl diphosphate is the main product, the C55-polyprenyl diphosphate and the C45-polyprenyl diphosphate are minor products. With 0.5% Triton X-100 the C50-polyprenyl diphosphate compound and the C45-compound are the main products, the C55-compound is not formed ?

Synonyms

Synonyms Comment Organism
undecaprenyl pyrophosphate synthetase
-
Escherichia coli
UPP synthetase
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 9
-
Escherichia coli