Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | no activity in absence | Escherichia coli | |
Triton X-100 | no activity in the activation by Triton X-100 is almost saturated at a concentration of 0.02% | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
farnesyl diphosphate | pH 7.5, 30°C | Escherichia coli | |
0.029 | - |
isopentenyl diphosphate | pH 7.5, 30°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ or Mn2+ required. Optimal concentration for Mg2+ is 1.0 mM | Escherichia coli | |
Mg2+ | or Mn2+ required. Optimal concentration 1.0 mM | Escherichia coli | |
Mn2+ | or Mg2+ required | Escherichia coli | |
Mn2+ | Mn2+ or Mg2+ required | Escherichia coli | |
Mn2+ | maximal stimulation at 0.05-0.1 mM, Mg2+ or Mn2+ required | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, activity in enzyme preparation is stable for several weeks | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
farnesyl diphosphate + 8 isopentenyl diphosphate | neither dimethylallyl diphosphate nor geranyl diphosphate act as priming substrate for the enzyme | Escherichia coli | 8 diphosphate + undecaprenyl diphosphate | - |
? | |
isopentenyl diphosphate + farnesyl diphosphate | - |
Escherichia coli | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | with 0.02% Triton X-100 the main product is the C55-compound, C50-compound is the minor product. With 0.1% Triton X-100 the C50-polyprenyl diphosphate is the main product, the C55-polyprenyl diphosphate and the C45-polyprenyl diphosphate are minor products. With 0.5% Triton X-100 the C50-polyprenyl diphosphate compound and the C45-compound are the main products, the C55-compound is not formed | ? |
Synonyms | Comment | Organism |
---|---|---|
undecaprenyl pyrophosphate synthetase | - |
Escherichia coli |
UPP synthetase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | - |
Escherichia coli |