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Literature summary for 2.5.1.30 extracted from
- Heptaprenyl pyrophosphate synthetase from Bacillus subtilis (1980), J. Biol. Chem., 255, 4539-4543.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0083 | - |
geranylgeranyl diphosphate | pH 7.5, 37°C | Bacillus subtilis |  |
| 0.0128 | - |
isopentenyl diphosphate | pH 7.5, 37°C | Bacillus subtilis | |
| 0.0133 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 37°C | Bacillus subtilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, no activation with Mn2+ | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
gel filtration | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | P31112 and P31114 | P31112: subunit 1, P31114: subunit 2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate | the enzyme reaction proceeds with an elimination of 2-pro-R hydrogen of isopentenyl diposphate without accumulation of any prenyl diphosphate shorter than C35 | Bacillus subtilis | 4 diphosphate + all-trans-heptaprenyl diphosphate | - |
? | |
| geranylgeranyl diphosphate + 3 isopentenyl diphosphate | the enzyme reaction proceeds with an elimination of 2-pro-R hydrogen of isopentenyl diphosphate without accumulation of any prenyl diphosphate shorter than C35. Poor activity of geranylneryl diphosphate contrast with the high activity of the all-trans isomer is consistent with the proposal that this enzyme catalyzes condensations resulting in trans products | Bacillus subtilis | 3 diphosphate + all-trans-heptaprenyl diphosphate | - |
? | |
| additional information | the enzyme does not catalyze a reaction between isopentenyl diphosphate and either dimethylallyl or geranyl diphosphate | Bacillus subtilis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| heptaprenyl pyrophosphate synthetase | - |
Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7 | - |
Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8 | pH 5.5: about 50% of maximal activity, pH 8.0: about 50% of maximal activity | Bacillus subtilis |
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