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Literature summary for 2.5.1.29 extracted from

  • Kajiura, H.; Suzuki, N.; Tokumoto, Y.; Yoshizawa, T.; Takeno, S.; Fujiyama, K.; Kaneko, Y.; Matsumura, H.; Nakazawa, Y.
    Two Eucommia farnesyl diphosphate synthases exhibit distinct enzymatic properties leading to end product preferences (2017), Biochimie, 139, 95-106 .
    View publication on PubMed
Search for more literature for 2.5.1.29

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as His-tagged protein Eucommia ulmoides

Inhibitors

Inhibitors Comment Organism Structure
isopentenyl diphosphate
-
 Eucommia ulmoides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0082
-
 dimethylallyl diphosphate fixed substrate: isopentenyl diphosphate, enzyme EuFPS2, pH 7.0, 25°C Eucommia ulmoides
0.0097
-
 geranyl diphosphate fixed substrate: isopentenyl diphosphate, enzyme EuFPS1, pH 7.0, 25°C Eucommia ulmoides
0.011
-
 geranyl diphosphate fixed substrate: isopentenyl diphosphate, enzyme EuFPS2, pH 7.0, 25°C Eucommia ulmoides
0.0146
-
 isopentenyl diphosphate fixed substrate: dimethylallyl diphosphate, enzyme EuFPS2, pH 7.0, 25°C Eucommia ulmoides
0.0202
-
 isopentenyl diphosphate fixed substrate: farnesyl diphosphate, enzyme EuFPS2, pH 7.0, 25°C Eucommia ulmoides
0.028
-
 isopentenyl diphosphate fixed substrate: farnesyl diphosphate, enzyme EuFPS1, pH 7.0, 25°C Eucommia ulmoides
0.0298
-
 isopentenyl diphosphate fixed substrate: dimethylallyl diphosphate, enzyme EuFPS1, pH 7.0, 25°C Eucommia ulmoides
0.0302
-
 isopentenyl diphosphate fixed substrate: dimethylallyl diphosphate, enzyme EuFPS2, pH 7.0, 25°C Eucommia ulmoides
0.0309
-
 dimethylallyl diphosphate fixed substrate: isopentenyl diphosphate, enzyme EuFPS1, pH 7.0, 25°C Eucommia ulmoides
0.0483
-
 isopentenyl diphosphate fixed substrate: dimethylallyl diphosphate, enzyme EuFPS1, pH 7.0, 25°C Eucommia ulmoides
0.187
-
 (2E,6E)-farnesyl diphosphate fixed substrate: isopentenyl diphosphate, enzyme EuFPS2, pH 7.0, 25°C Eucommia ulmoides
0.275
-
 (2E,6E)-farnesyl diphosphate fixed substrate: isopentenyl diphosphate, enzyme EuFPS1, pH 7.0, 25°C Eucommia ulmoides

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the enzyme requires Mg2+ and is inactive in the absence of metal. Optimal concentration of Mg2+ is 0.1 mM. Co2+ and Mn2+ activate the synthesis of farnesyl diphosphat at lower concentrations (0.005-0.05 mM) than does Mg2+ (0.05-0.5 mM). The enzyme also utilizes Ca2+, Ni2+, and Zn2+ as cofactors, but only at low concentrations (0.001-0.05 mM) Eucommia ulmoides
Co2+ the enzyme requires Mg2+ and is inactive in the absence of metal. Optimal concentration of Mg2+ is 0.1 mM. Co2+ and Mn2+ activate the synthesis of farnesyl diphosphat at lower concentrations (0.005-0.05 mM) than does Mg2+ (0.05-0.5 mM). The enzyme also utilizes Ca2+, Ni2+, and Zn2+ as cofactors, but only at low concentrations (0.001-0.05 mM) Eucommia ulmoides
Mg2+ the enzyme requires Mg2+ and is inactive in the absence of metal. Optimal concentration of Mg2+ is 0.1 mM. Co2+ and Mn2+ activate the synthesis of farnesyl diphosphat at lower concentrations (0.005-0.05 mM) than does Mg2+ (0.05-0.5 mM). The enzyme also utilizes Ca2+, Ni2+, and Zn2+ as cofactors, but only at low concentrations (0.001-0.05 mM) Eucommia ulmoides
Mn2+ the enzyme requires Mg2+ and is inactive in the absence of metal. Optimal concentration of Mg2+ is 0.1 mM. Co2+ and Mn2+ activate the synthesis of farnesyl diphosphat at lower concentrations (0.005-0.05 mM) than does Mg2+ (0.05-0.5 mM). The enzyme also utilizes Ca2+, Ni2+, and Zn2+ as cofactors, but only at low concentrations (0.001-0.05 mM) Eucommia ulmoides
Ni2+ the enzyme requires Mg2+ and is inactive in the absence of metal. Optimal concentration of Mg2+ is 0.1 mM. Co2+ and Mn2+ activate the synthesis of farnesyl diphosphat at lower concentrations (0.005-0.05 mM) than does Mg2+ (0.05-0.5 mM). The enzyme also utilizes Ca2+, Ni2+, and Zn2+ as cofactors, but only at low concentrations (0.001-0.05 mM) Eucommia ulmoides
Zn2+ the enzyme requires Mg2+ and is inactive in the absence of metal. Optimal concentration of Mg2+ is 0.1 mM. Co2+ and Mn2+ activate the synthesis of farnesyl diphosphat at lower concentrations (0.005-0.05 mM) than does Mg2+ (0.05-0.5 mM). The enzyme also utilizes Ca2+, Ni2+, and Zn2+ as cofactors, but only at low concentrations (0.001-0.05 mM) Eucommia ulmoides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 SDS-PAGE Eucommia ulmoides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate Eucommia ulmoides
-
 diphosphate + geranylgeranyl diphosphate
-
 ?
dimethylallyl diphosphate + isopentenyl diphosphate Eucommia ulmoides
-
 diphosphate + geranyl diphosphate
-
 ?
geranyl diphosphate + isopentenyl diphosphate Eucommia ulmoides
-
 diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Eucommia ulmoides Q94IE8
-
-
Eucommia ulmoides Q94IE9
-
-

Purification (Commentary)

Purification (Comment) Organism
purified by Co2+ affinity chromatography Eucommia ulmoides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate
-
 Eucommia ulmoides diphosphate + geranylgeranyl diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate highest specific activity is observed using geranyl diphosphate as an allylic substrate. For all allylic substrates tested, EuFPS2 shows higher chain elongation activity than does EuFSP1. Depending on the pH, the metal ion cofactor, and the cofactor concentration, EuFPS2 accumulates geranyl diphosphate as an intermediate product at a constant rate, whereas EuFPS1 synthesizes little geranyl diphosphate Eucommia ulmoides diphosphate + geranylgeranyl diphosphate
-
 ?
dimethylallyl diphosphate + isopentenyl diphosphate
-
 Eucommia ulmoides diphosphate + geranyl diphosphate
-
 ?
dimethylallyl diphosphate + isopentenyl diphosphate highest specific activity is observed using geranyl diphosphate as an allylic substrate. For all allylic substrates tested, EuFPS2 shows higher chain elongation activity than does EuFSP1. Depending on the pH, the metal ion cofactor, and the cofactor concentration, EuFPS2 accumulates geranyl diphosphate as an intermediate product at a constant rate, whereas EuFPS1 synthesizes little geranyl diphosphate Eucommia ulmoides diphosphate + geranyl diphosphate
-
 ?
geranyl diphosphate + isopentenyl diphosphate
-
 Eucommia ulmoides diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?
geranyl diphosphate + isopentenyl diphosphate highest specific activity is observed using geranyl diphosphate as an allylic substrate. For all allylic substrates tested, EuFPS2 shows higher chain elongation activity than does EuFSP1. Depending on the pH, the metal ion cofactor, and the cofactor concentration, EuFPS2 accumulates geranyl diphosphate as an intermediate product at a constant rate, whereas EuFPS1 synthesizes little geranyl diphosphate Eucommia ulmoides diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
EuFPS1
-
 Eucommia ulmoides
EuFPS2
-
 Eucommia ulmoides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20 30 enzyme EuFPS1 Eucommia ulmoides
20 30 enzyme EuFPS2 Eucommia ulmoides

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 1 h, stable. Activity dramatically decreases at higher temperatures Eucommia ulmoides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 10 enzyme EuFPS1 Eucommia ulmoides
7 9.5 enzyme EuFPS2 Eucommia ulmoides

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.102
-
 isopentenyl diphosphate fixed substrate: dimethylallyl diphosphate, enzyme EuFPS1, substrate: dimethylallyl diphosphate, fixed substrate: isopentenyl diphosphate, enzyme EuFPS1, pH 7.0, 25°C Eucommia ulmoides
0.1052
-
 isopentenyl diphosphate fixed substrate: dimethylallyl diphosphate, enzyme EuFPS2, substrate: isopentenyl diphosphate, fixed substrate: dimethylallyl diphosphate, enzyme EuFPS2, pH 7.0, 25°C Eucommia ulmoides
0.1187
-
 isopentenyl diphosphate fixed substrate: dimethylallyl diphosphate, enzyme EuFPS1, substrate: dimethylallyl diphosphate, fixed substrate: isopentenyl diphosphate, enzyme EuFPS2, pH 7.0, 25°C Eucommia ulmoides
0.1264
-
 isopentenyl diphosphate fixed substrate: dimethylallyl diphosphate, enzyme EuFPS1, substrate: isopentenyl diphosphate, fixed substrate: dimethylallyl diphosphate, enzyme EuFPS1, pH 7.0, 25°C Eucommia ulmoides
0.2249
-
 isopentenyl diphosphate fixed substrate: dimethylallyl diphosphate, enzyme EuFPS1, substrate: isopentenyl diphosphate, fixed substrate: geranyl diphosphate, enzyme EuFPS2, pH 7.0, 25°C Eucommia ulmoides
0.2373
-
 isopentenyl diphosphate fixed substrate: dimethylallyl diphosphate, enzyme EuFPS1, substrate: isopentenyl diphosphate, fixed substrate: farnesyl diphosphate, enzyme EuFPS1, pH 7.0, 25°C Eucommia ulmoides
0.2473
-
 isopentenyl diphosphate fixed substrate: dimethylallyl diphosphate, enzyme EuFPS1, substrate: isopentenyl diphosphate, fixed substrate: geranyl diphosphate, enzyme EuFPS1, pH 7.0, 25°C Eucommia ulmoides
0.2673
-
 isopentenyl diphosphate fixed substrate: dimethylallyl diphosphate, enzyme EuFPS1, substrate: isopentenyl diphosphate, fixed substrate: farnesyl diphosphate, enzyme EuFPS2, pH 7.0, 25°C Eucommia ulmoides

General Information

General Information Comment Organism
physiological function essential enzyme in the biosynthesis of prenyl precursors for the production of primary and secondary metabolites, including sterols, dolichols, carotenoids and ubiquinones, and for the modification of proteins Eucommia ulmoides