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Literature summary for 2.5.1.29 extracted from

  • Schmidt, A.; Waechtler, B.; Temp, U.; Krekling, T.; Seguin, A.; Gershenzon, J.
    A bifunctional geranyl and geranylgeranyl diphosphate synthase is involved in terpene oleoresin formation in Picea abies (2010), Plant Physiol., 152, 639-655.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli and in Picea abies embryogenic tissue Picea abies

Protein Variants

Protein Variants Comment Organism
DelP173/DelP174 decrease in reaction rate Picea abies
G257D/P174C little effect on reation rate, no effect on product composition Picea abies
I161M little effect on reation rate, no effect on product composition Picea abies
I235T decrease in reation rate, single product is geranyldiphosphate Picea abies
L180F little effect on reation rate, product composition is about 98% geranyldiphosphate, 12% geranygeranyl diphosphate Picea abies
L180F/P174C little effect on reation rate, product composition is about 98% geranyldiphosphate, 12% geranygeranyl diphosphate Picea abies
L273F decrease in reation rate, single product is geranyl diphosphate Picea abies
M159I little effect on reation rate, no effect on product composition Picea abies
M165C little effect on reation rate, no effect on product composition Picea abies
M165Y little effect on reation rate, product composition is about 90% geranyldiphosphate, 10% farnesyl diphosphate Picea abies
M175I little effect on reation rate, product composition is about 95% geranyldiphosphate, 3% farnesyl diphosphate, 25% geranygeranyl diphosphate Picea abies
M175I/P174C little effect on reation rate, single product is geranyldiphosphate Picea abies
M175I/P174S little effect on reation rate, no effect on product composition Picea abies
P174C little effect on reation rate, no effect on product composition Picea abies
P174S little effect on reation rate, no effect on product composition Picea abies
V227M decrease in reation rate Picea abies
V240L decrease in reation rate, no effect on product composition Picea abies

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.39
-
dimethylallyl diphosphate
-
Picea abies

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, activity reaches its highest value at 10 mM MgCl2. At 5 or 15 mM MgCl2, activity is about 65% of maximum Picea abies

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
x * 40000, SDS-PAGE of recombinant protein Picea abies

Organism

Organism UniProt Comment Textmining
Picea abies D2IT07 bifunctional geranyl diphosphate synthase and geranylgeranyl diphosphate synthase
-

Source Tissue

Source Tissue Comment Organism Textmining
bark of sapling Picea abies
-
epithelial cell protein is localized to epithelial cells surrounding the traumatic resin ducts Picea abies
-
sapling expression is restricted to wood and bark Picea abies
-
xylem of sapling Picea abies
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + 3 isopentenyl diphosphate
-
Picea abies diphosphate + geranylgeranyl diphosphate geranyl diphosphate activity of enzyme leads to additional production of geranyl diphosphate. The ratio of geranyl diphosphate to geranylgeranyl diphosphate produced averages approximately 9:1. No synthesis of farnesyl diphosphate ?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE of recombinant protein Picea abies

Synonyms

Synonyms Comment Organism
isoprenyl diphosphate synthase
-
Picea abies

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4
-
dimethylallyl diphosphate
-
Picea abies

Expression

Organism Comment Expression
Picea abies treatment with methyl jasmonate dramatically increases expression level in sapling up