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Literature summary for 2.5.1.29 extracted from

  • Matsumura, Y.; Kidokoro, T.; Miyagi, Y.; Marilingaiah, N.R.; Sagami, H.
    The carboxyl-terminal region of the geranylgeranyl diphosphate synthase is indispensable for the stabilization of the region involved in substrate binding and catalysis (2007), J. Biochem., 142, 533-537.
    View publication on PubMed
Search for more literature for 2.5.1.29

Cloned(Commentary)

Cloned (Comment) Organism
the histidine-tagged deletion mutants (from the carboxyl terminus) (DELTA-4, DELTA-8, DELTA-12 and DELTa-16) are overexpressed in Escherichia coli BL21 (DE3) and purified in a stable form by nickel affinity chromatography except for one mutant DELTA-16 Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information the farnesyl-transferring activities of wild-type GGPS, DELTA-4, DELTA-8 and DELTA-12 carboxyterminal deletion mutants are relatively in a ratio of 1.0, 0.84, 0.26 and 0.0015. Each Km value of the four recombinants are estimated to be 0.00071, 0.002, 0.0028 and 0.055 mM for farnesyl diphosphate and to be 0.0029, 0.0051, 0.056 and above 0.1 mM for isopentenyl diphosphate, respectively. Allylic substrate specificities of these recombinants are estimated by quantitative analysis of the products, revealing that DELTA-8 and DELTA-12 mutants lack the ability to accept dimethylallyl and geranyl diphosphates compared to wild-type GGPS and DELTA-4 mutant Rattus norvegicus

General Stability

General Stability Organism
KMFTEENE residing on the carboxyl-terminal sequence of GGPS stabilizes the active region involved in the substrate binding and catalysis Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00071
-
 trans,trans-farnesyl diphosphate pH 7.0, 37°C, wild-type enzyme Rattus norvegicus
0.002
-
 trans,trans-farnesyl diphosphate pH 7.0, 37°C, carboxy-terminal deletion mutant DELTA-4 Rattus norvegicus
0.0028
-
 trans,trans-farnesyl diphosphate pH 7.0, 37°C, carboxy-terminal deletion mutant DELTA-8 Rattus norvegicus
0.0029
-
 isopentenyl diphosphate pH 7.0, 37°C, wild-type enzyme Rattus norvegicus
0.0051
-
 isopentenyl diphosphate pH 7.0, 37°C, carboxy-terminal deletion mutant DELTA-4 Rattus norvegicus
0.055
-
 trans,trans-farnesyl diphosphate pH 7.0, 37°C, carboxy-terminal deletion mutant DELTA-12 Rattus norvegicus
0.056
-
 isopentenyl diphosphate pH 7.0, 37°C, carboxy-terminal deletion mutant DELTA-8 Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
the histidine-tagged deletion mutants (from the carboxyl terminus) (DELTA-4, DELTA-8, DELTA-12 and DELTa-16) are overexpressed in Escherichia coli BL21 (DE3) and purified in a stable form by nickel affinity chromatography except for one mutant DELTA-16 Rattus norvegicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000176
-
 carboxy-terminal deletion mutant DELTA-8 Rattus norvegicus
0.0306
-
 carboxy-terminal deletion mutant DELTA-8 Rattus norvegicus
0.0983
-
 carboxy-terminal deletion mutant DELTA-4 Rattus norvegicus
0.117
-
 wild-type enzyme Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trans,trans-farnesyl diphosphate + isopentenyl diphosphate
-
 Rattus norvegicus diphosphate + geranylgeranyl diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
geranylgeranyl diphosphate synthase
-
 Rattus norvegicus
GGPS
-
 Rattus norvegicus