Activating Compound | Comment | Organism | Structure |
---|---|---|---|
octyl glucoside | 0.8%, 1.2fold activation | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(E,E,E)-geranylgeranyl diphosphate | 0.005 mM, 50% inhibition, competitive vs. farnesyl diphosphate | Bos taurus | |
(Z,E,E)-Geranylgeranyl diphosphate | 0.005 mM, approx. 50% inhibition, competitive vs. farnesyl diphosphate | Bos taurus | |
3-azageranylgeranyl diphosphate | - |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00074 | - |
farnesyl diphosphate | - |
Bos taurus | |
0.0008 | - |
geranyl diphosphate | - |
Bos taurus | |
0.002 | - |
isopentenyl diphosphate | - |
Bos taurus | |
0.033 | - |
dimethylallyl diphosphate | - |
Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | maximal activity at 1 mM | Bos taurus | |
Mg2+ | required for activity | Bos taurus | |
Mn2+ | required for activity | Bos taurus | |
Mn2+ | maximal activity at 1 mM, less effective than Mg2+ | Bos taurus | |
Zn2+ | maximal activity at 1 mM, approx. 30% of activation with Mg2+ | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37500 | - |
x * 37500, tetramer or pentamer, SDS-PAGE | Bos taurus |
150000 | - |
Superose 12, gel filtration | Bos taurus |
190000 | - |
Superdex 200, gel filtration | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E,E)-farnesyl diphosphate + isopentenyl diphosphate | Bos taurus | - |
geranylgeranyl diphosphate + diphosphate | - |
? | |
geranyl diphosphate + 2 isopentenyl diphosphate | Bos taurus | - |
geranylgeranyl diphosphate + 2 diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, farnesylmethyl affinity gel | Bos taurus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.098 | - |
- |
Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E,E)-farnesyl diphosphate + isopentenyl diphosphate | - |
Bos taurus | geranylgeranyl diphosphate + diphosphate | - |
? | |
(E,E)-farnesyl diphosphate + isopentenyl diphosphate | - |
Bos taurus | geranylgeranyl diphosphate + diphosphate | exclusive product | ? | |
dimethylallyl diphosphate + 3 isopentenyl diphosphate | - |
Bos taurus | geranylgeranyl diphosphate + 3 diphosphate | - |
? | |
geranyl diphosphate + 2 isopentenyl diphosphate | - |
Bos taurus | geranylgeranyl diphosphate + 2 diphosphate | - |
? | |
geranyl diphosphate + 2 isopentenyl diphosphate | - |
Bos taurus | geranylgeranyl diphosphate + 2 diphosphate | geranylgeranyl diphosphate + farnesyl diphosphate | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37500, tetramer or pentamer, SDS-PAGE | Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
(Z,E,E)-Geranylgeranyl diphosphate | - |
Bos taurus | |
0.0012 | - |
(E,E,E)-geranylgeranyl diphosphate | - |
Bos taurus |