Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.29 extracted from

  • Wiedemann, M.; Misawa, N.; Sandmann, G.
    Purification and enzymatic characterization of the geranylgeranyl pyrophosphate synthase from Erwinia uredovora after expression in Escherichia coli (1993), Arch. Biochem. Biophys., 306, 152-157.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pantoea ananatis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
geranyl diphosphate
-
Pantoea ananatis
0.011
-
farnesyl diphosphate
-
Pantoea ananatis
0.036
-
isopentenyl diphosphate
-
Pantoea ananatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(E,E)-farnesyl diphosphate + isopentenyl diphosphate Pantoea ananatis
-
geranylgeranyl diphosphate + diphosphate
-
?
dimethylallyl diphosphate + 3 isopentenyl diphosphate Pantoea ananatis
-
geranylgeranyl diphosphate + 3 diphosphate
-
?
geranyl diphosphate + 2 isopentenyl diphosphate Pantoea ananatis
-
geranylgeranyl diphosphate + 2 diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Pantoea ananatis
-
overexpressed in Escherichia coli
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, urea, DEAE-52 Pantoea ananatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E,E)-farnesyl diphosphate + isopentenyl diphosphate
-
Pantoea ananatis geranylgeranyl diphosphate + diphosphate
-
?
dimethylallyl diphosphate + 3 isopentenyl diphosphate
-
Pantoea ananatis geranylgeranyl diphosphate + 3 diphosphate
-
?
farnesyl diphosphate + isopentenyl diphosphate
-
Pantoea ananatis diphosphate + geranylgeranyl diphosphate
-
?
geranyl diphosphate + 2 isopentenyl diphosphate
-
Pantoea ananatis geranylgeranyl diphosphate + 2 diphosphate
-
?