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Literature summary for 2.5.1.29 extracted from

  • Ogura, K.; Nishino, T.; Shinka, T.; Seto, S.
    Prenyltransferases of pumpkin fruit (1985), Methods Enzymol., 110, 167-171.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0017
-
farnesyl diphosphate
-
Cucurbita pepo

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Cucurbita pepo
Mg2+ less effective than Mn2+ Cucurbita pepo
Mn2+ activates much more effectively than Mg2+ Cucurbita pepo

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(E,E)-farnesyl diphosphate + isopentenyl diphosphate Cucurbita pepo
-
geranylgeranyl diphosphate + diphosphate
-
?
geranyl diphosphate + 2 isopentenyl diphosphate Cucurbita pepo
-
geranylgeranyl diphosphate + 2 diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Cucurbita pepo
-
pumpkin
-

Purification (Commentary)

Purification (Comment) Organism
-
Cucurbita pepo

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
Cucurbita pepo
-

Storage Stability

Storage Stability Organism
-20°C, at least 1 week, no loss of activity Cucurbita pepo

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E,E)-farnesyl diphosphate + isopentenyl diphosphate
-
Cucurbita pepo geranylgeranyl diphosphate + diphosphate
-
?
(E,E)-farnesyl diphosphate + isopentenyl diphosphate
-
Cucurbita pepo geranylgeranyl diphosphate + diphosphate E,E)-geranylgeranyl diphosphate ?
3-methyl-2-alkenyl diphosphate + isopentenyl diphosphate a number of 3-methyl-2-alkenyl diphosphates ranging in carbon number from 6 to 13 act as substrates Cucurbita pepo ?
-
?
geranyl diphosphate + 2 isopentenyl diphosphate
-
Cucurbita pepo geranylgeranyl diphosphate + 2 diphosphate
-
?
geranyl diphosphate + 2 isopentenyl diphosphate
-
Cucurbita pepo geranylgeranyl diphosphate + 2 diphosphate E,E)-geranylgeranyl diphosphate ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Cucurbita pepo