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Literature summary for 2.5.1.29 extracted from
- Geranylpyrophosphate synthetase-geranylgeranylpyrophosphate synthetase from Micrococcus luteus (1985), Methods Enzymol., 110, 188-192.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | stimulates farnesyl-transferring activity, inhibits dimethylallyl-transferring activity | Micrococcus luteus |  |
| Tween 80 | stimulates | Micrococcus luteus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diphosphate | - |
Micrococcus luteus | |
| farnesyl diphosphate | - |
Micrococcus luteus | |
| Triton X-100 | stimulates farnesyl-transferring activity, inhibits dimethylallyl-transferring activity | Micrococcus luteus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity | Micrococcus luteus | |
| Mg2+ | optimal activation of farnesyl-transferring activity at 1 mM, optimal activation of dimethylallyl-transferring activity at 3 mM | Micrococcus luteus | |
| Mn2+ | slight activation | Micrococcus luteus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
gel filtration | Micrococcus luteus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (E,E)-farnesyl diphosphate + isopentenyl diphosphate | Micrococcus luteus | - |
geranylgeranyl diphosphate + diphosphate | - |
? | |
| geranyl diphosphate + 2 isopentenyl diphosphate | Micrococcus luteus | - |
geranylgeranyl diphosphate + 2 diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Micrococcus luteus | - |
the activities of EC 2.5.1.1 and EC 2.5.1.29 may be a mixture of 2 enzymes or a single enzyme with two independent catalytic sites, see EC 2.5.1.1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Micrococcus luteus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, at least 1 week, no loss of activity | Micrococcus luteus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (E)-3-methyl-2-dodecenyl diphosphate + isopentenyl diphosphate | 45% of the activity with farnesyl diphosphate | Micrococcus luteus | (2E,5E)-3,6-dimethylpentadeca-2,5-dien-1-yl diphosphate + diphosphate | - |
? | |
| (E,E)-farnesyl diphosphate + isopentenyl diphosphate | - |
Micrococcus luteus | geranylgeranyl diphosphate + diphosphate | - |
? | |
| E-3-methyl-2-undecenyl diphosphate + isopentenyl diphosphate | 53% of the activity with farnesyl diphosphate | Micrococcus luteus | ? | - |
? | |
| geranyl diphosphate + 2 isopentenyl diphosphate | - |
Micrococcus luteus | geranylgeranyl diphosphate + 2 diphosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.7 | - |
- |
Micrococcus luteus |
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Release 2023.1 (January 2023)
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