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Literature summary for 2.5.1.27 extracted from

  • Yeh, C.; Hsieh, L.; Yang, C.; Lee, P.
    Molecular characterization of isopentenyltransferase (BoAIPT1) from Bambusa oldhamii expressed in Escherichia coli (2011), Bot. Stud., 52, 249-256.
No PubMed abstract available
Search for more literature for 2.5.1.27

Cloned(Commentary)

Cloned (Comment) Organism
cDNA encoding BoAIPT1, DNA and amino acid sequence determination and analysis, sequence comparison, functional expression of N-terminally His6-tagged BoAIPT1 in Escherichia coli strain BL21 (DE3), optimum protein expression is obtained by incubating the IPTG-induced culture at 18°C for 18 hr, transgenic expression in bamboo plants, real-time PCR expression analysis Bambusa oldhamii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Bambusa oldhamii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 x * 40000, recombinant His6-tagged BoAIPT1, SDS-PAGE Bambusa oldhamii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dimethylallyl diphosphate + AMP Bambusa oldhamii
-
 diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Bambusa oldhamii D7R812
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged BoAIPT1 from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and ultrafiltration Bambusa oldhamii

Source Tissue

Source Tissue Comment Organism Textmining
shoot
-
 Bambusa oldhamii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + AMP
-
 Bambusa oldhamii diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, recombinant His6-tagged BoAIPT1, SDS-PAGE Bambusa oldhamii

Synonyms

Synonyms Comment Organism
adenylate isopentenyltransferase
-
 Bambusa oldhamii
AIPT1
-
 Bambusa oldhamii
isopentenyltransferase
-
 Bambusa oldhamii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Bambusa oldhamii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Bambusa oldhamii

General Information

General Information Comment Organism
additional information transgenic expression of BoAIPT1 in bamboo plants increases the green bamboo shoot growth, with the top green shoot section having the highest expression level Bambusa oldhamii