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Literature summary for 2.5.1.21 extracted from

  • Zhang, B.; Liu, Y.; Chen, M.; Feng, J.; Ma, Z.; Zhang, X.; Zhu, C.
    Cloning, expression analysis and functional characterization of squalene synthase (SQS) from Tripterygium wilfordii (2018), Molecules, 23, 269 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.21

Cloned(Commentary)

Cloned (Comment) Organism
the truncated squalene synthase without the last 28 amino acids of the carboxy terminus is inductively expressed in Escherichia coli Transetta (DE3) Tripterygium wilfordii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Tripterygium wilfordii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ Tripterygium wilfordii the enzyme is involved in celastrol biosynthesis squalene + 2 diphosphate + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Tripterygium wilfordii A0A142IKD0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Tripterygium wilfordii

Source Tissue

Source Tissue Comment Organism Textmining
leaf highly expressed in roots, followed by the stems and leaves Tripterygium wilfordii
-
root highly expressed in roots, followed by the stems and leaves Tripterygium wilfordii
-
stem highly expressed in roots, followed by the stems and leaves Tripterygium wilfordii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ overall reaction Tripterygium wilfordii squalene + 2 diphosphate + NADP+
-
 ?
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ the enzyme is involved in celastrol biosynthesis Tripterygium wilfordii squalene + 2 diphosphate + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
SQS
-
 Tripterygium wilfordii

Expression

Organism Comment Expression
Tripterygium wilfordii expression is significantly up-regulated upon methyl jasmonate treatment up

General Information

General Information Comment Organism
metabolism the enzyme is involved in celastrol biosynthesis Tripterygium wilfordii