Literature summary for 2.5.1.21 extracted from

Su, H.; Liu, Y.; Xiao, Y.; Tan, Y.; Gu, Y.; Liang, B.; Huang, H.; Wu, Y.
Molecular and biochemical characterization of squalene synthase from Siraitia grosvenorii (2017), Biotechnol. Lett., 39, 1009-1018 .

Search for more literature for 2.5.1.21

Cloned(Commentary)

Cloned (Comment)	Organism
full-length SgSQS with measurable catalytic activity is expressed in Escherichia coli	Siraitia grosvenorii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0055	-	(2E,6E)-farnesyl diphosphate	pH 7.4, 37°C	Siraitia grosvenorii
0.099	-	NADPH	pH 7.4, 37°C	Siraitia grosvenorii

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	the enzyme has two transmembrane domains at its C-terminus	Siraitia grosvenorii	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2 (2E,6E)-farnesyl diphosphate + NADPH + H+	Siraitia grosvenorii	-	squalene + 2 diphosphate + NADP+	-	?

Organism

Organism	UniProt	Comment	Textmining
Siraitia grosvenorii	A0A192YD12	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
exocarp	mRNA expression in stem and root are about twice as much as that in leaf and peel	Siraitia grosvenorii	-
leaf	mRNA expression in stem and root are about twice as much as that in leaf and peel	Siraitia grosvenorii	-
root	mRNA expression in stem and root are about twice as much as that in leaf and peel	Siraitia grosvenorii	-
stem	mRNA expression in stem and root are about twice as much as that in leaf and peel	Siraitia grosvenorii	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 (2E,6E)-farnesyl diphosphate + NADPH + H+	-	Siraitia grosvenorii	squalene + 2 diphosphate + NADP+	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 48000, calculated from sequence	Siraitia grosvenorii

Synonyms

Synonyms	Comment	Organism
SgSQS	-	Siraitia grosvenorii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	-	Siraitia grosvenorii

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
25	50	25°C: about 70% of maximal activity, 50°C: about 55% of maximal activity	Siraitia grosvenorii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Siraitia grosvenorii

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Siraitia grosvenorii

pI Value

Organism	Comment	pI Value Maximum	pI Value
Siraitia grosvenorii	calculated from sequence	-	7.3

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Release 2023.1 (January 2023)