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Literature summary for 2.5.1.21 extracted from

  • Song, J.; Shang, N.; Baig, N.; Yao, J.; Shin, C.; Kim, B.K.; Li, Q.; Malwal, S.R.; Oldfield, E.; Feng, X.; Guo, R.T.
    Aspergillus flavus squalene synthase as an antifungal target Expression, activity, and inhibition (2019), Biochem. Biophys. Res. Commun., 512, 517-523 .
    View publication on PubMed
Search for more literature for 2.5.1.21

Application

Application Comment Organism
agriculture squalene synthase is an antifungal target Aspergillus flavus

Cloned(Commentary)

Cloned (Comment) Organism
-
 Aspergillus flavus

Inhibitors

Inhibitors Comment Organism Structure
BPH-652 potent inhibitor Aspergillus flavus
celastrol binds to the enzyme in a noncompetitive manner, but does not bind covalently. Celastrol also inhibits growth of the highly virulent Aspergillus fumigatus by inhibiting flavin-dependent monooxygenase siderophore A , it may be a promising multi-target lead for antifungal development Aspergillus flavus
zaragozic acid potent inhibitor Aspergillus flavus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 NADPH pH 7.4, temperature not specified in the publication Aspergillus flavus
0.061
-
 (2E,6E)-farnesyl diphosphate pH 7.4, temperature not specified in the publication Aspergillus flavus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
427178
-
 MALDI-TOF analysis Aspergillus flavus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ Aspergillus flavus
-
 squalene + 2 diphosphate + NADP+
-
 ?
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ Aspergillus flavus AF70
-
 squalene + 2 diphosphate + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus flavus A0A2P2GY60
-
-
Aspergillus flavus AF70 A0A2P2GY60
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus flavus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 (2E,6E)-farnesyl diphosphate + NADPH + H+
-
 Aspergillus flavus squalene + 2 diphosphate + NADP+
-
 ?
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ overall reaction Aspergillus flavus squalene + 2 diphosphate + NADP+
-
 ?
2 (2E,6E)-farnesyl diphosphate + NADPH + H+
-
 Aspergillus flavus AF70 squalene + 2 diphosphate + NADP+
-
 ?
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ overall reaction Aspergillus flavus AF70 squalene + 2 diphosphate + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
SQS
-
 Aspergillus flavus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.027
-
 (2E,6E)-farnesyl diphosphate pH 7.4, temperature not specified in the publication Aspergillus flavus
0.039
-
 NADPH pH 7.4, temperature not specified in the publication Aspergillus flavus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Aspergillus flavus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00083
-
 pH 7.4, temperature not specified in the publication Aspergillus flavus celastrol