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Literature summary for 2.5.1.21 extracted from
- Cloning, expression and purification of squalene synthase from Candida tropicalis in Pichia pastoris (2014), Protein Expr. Purif., 94, 15-21.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ERG9, recombinant expression of C-terminally His6-tagged enzyme in Pichia pastoris strain GS115 | Candida tropicalis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | heterologous expression of antigenic enzyme from Candida tropicalis in Pichia pastoris can be exploited for large-scale production | Candida tropicalis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Candida tropicalis | 16020 | - |
| microsome | - |
Candida tropicalis | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
- |
Candida tropicalis |
| 57000 | - |
- |
Candida tropicalis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 (2E,6E)-farnesyl diphosphate + NADPH + H+ | Candida tropicalis | - |
squalene + 2 diphosphate + NADP+ | - |
? |  |
| 2 (2E,6E)-farnesyl diphosphate + NADPH + H+ | Candida tropicalis ATCC MYA-3404 | - |
squalene + 2 diphosphate + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida tropicalis | C5M7M6 | gene ERG9 | - |
| Candida tropicalis ATCC MYA-3404 | C5M7M6 | gene ERG9 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Pichia pastoris strain GS115 by metal affinity chromatography to 97% purity | Candida tropicalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 (2E,6E)-farnesyl diphosphate + NADPH + H+ | - |
Candida tropicalis | squalene + 2 diphosphate + NADP+ | - |
? | |
| 2 (2E,6E)-farnesyl diphosphate + NADPH + H+ | - |
Candida tropicalis ATCC MYA-3404 | squalene + 2 diphosphate + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 57000, recombinant His6-tagged enzyme, SDS-PAGE, x * 55000, about, sequence calculation of His6-tagged enzyme | Candida tropicalis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SQS | - |
Candida tropicalis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Candida tropicalis | |
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