Application | Comment | Organism |
---|---|---|
medicine | squalene synthase is a potential target for antilipogenic and antiinfective therapies | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
gene fragment SQS(31-370) as N-terminally His6-tagged protein containing a thrombin cleavage site in Escherichia coli strain BL21(DE3) | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant truncated detagged SQS(31-370) in complex with inhibitor zaragozic acid A, vapour diffusion, mixing an equal volume of protein solution containing 15 mg/ml protein with precipitating solution containing 20% PEG2K-MME, 0.01 M NiCl2, 0.1 M Tris, pH 8.5, 1.4 M sodium citrate tribasic dehydrate, 0.1 M Na-HEPES, pH 7.5, 2 M K2HPO4/NaH2HPO4, pH 6.5, room temperature, X-ray diffraction structure determination and analysis, molecular replacement | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
zaragozic acid A | enzyme binding induces a local conformational change in the substrate binding site, and its C-6 acyl group also extends over to the cofactor binding cavity, enzyme-inhibitor binding structure and thermodynamics, detailed overview | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant truncated N-terminally His6-tagged SQS(31-370) from Escherichia coli strain BL21(DE3), the tag is cleaved off by thrombin | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 (2E,6E)-farnesyl diphosphate + NAD(P)H + H+ = squalene + 2 diphosphate + NAD(P)+ | overall reaction, reaction mechanism, overview | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 (2E,6E)-farnesyl diphosphate | - |
Homo sapiens | diphosphate + presqualene diphosphate | - |
? | |
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ | overall reaction | Homo sapiens | squalene + 2 diphosphate + NADP+ | - |
? | |
presqualene diphosphate + NADPH + H+ | - |
Homo sapiens | squalene + diphosphate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SQS | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | region IV of SQS may constitute a functional NADPH binding site | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | modifications in region IV prevents SQS from undergoing the second half-reaction, indicating that this region may reasonably constitute a functional NADPH binding site | Homo sapiens |
metabolism | squalene synthase catalyzes the committed step of sterol synthesis | Homo sapiens |