Application | Comment | Organism |
---|---|---|
drug development | LdSSN is a potential drug target for visceral leishmaniasis | Leishmania donovani |
Cloned (Comment) | Organism |
---|---|
LdSSN DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis, expression as C-terminally His6-tagged protein in Escherichia coli strain BL21 (DE3) | Leishmania donovani |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Guanidinium chloride | loss of 86% activity at 0.3M GdmCl, 50% at 0.2 M | Leishmania donovani | |
Urea | loss of 81% activity at 2 M, 50% at 1 M | Leishmania donovani | |
zaragozic acid A | is a potent inhibitor of mammalian SSN and also a competitive inhibitor of recombinant Leishmania donovani SSN, 50% inhibition at 100 nM | Leishmania donovani |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, recombinant LdSSN, overview | Leishmania donovani | |
0.0038 | - |
(2E,6E)-farnesyl diphosphate | pH 7.4, 37°C | Leishmania donovani | |
0.04323 | - |
NADPH | pH 7.4, 37°C | Leishmania donovani |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Leishmania donovani |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ | Leishmania donovani | overall reaction | squalene + 2 diphosphate + NADP+ | - |
? | |
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ | Leishmania donovani MHOM/IN/80/Dd8 | overall reaction | squalene + 2 diphosphate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania donovani | Q257D4 | - |
- |
Leishmania donovani MHOM/IN/80/Dd8 | Q257D4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged LdSSN from Escherichia coli strain BL21(DE3) by anion exchange chromatography, nickel affinity chrmatography is not successful | Leishmania donovani |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ | overall reaction | Leishmania donovani | squalene + 2 diphosphate + NADP+ | - |
? | |
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ | overall reaction | Leishmania donovani MHOM/IN/80/Dd8 | squalene + 2 diphosphate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | sequence comparison, overview | Leishmania donovani |
Synonyms | Comment | Organism |
---|---|---|
SSN | - |
Leishmania donovani |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Leishmania donovani |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 45 | 83% and 88% activity at a temperature of 30°C and 45°C | Leishmania donovani |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
purified recombinant enzyme, pH 7.4, loss of 85% activity | Leishmania donovani |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Leishmania donovani |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | activity range, over 90% of maximal activity at pH 7.0-8.0, 30% at pH 6.0, 20% at pH 9.0 | Leishmania donovani |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)H | - |
Leishmania donovani |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000074 | - |
zaragozic acid A | pH 7.4, 37°C | Leishmania donovani |
General Information | Comment | Organism |
---|---|---|
metabolism | squalene synthase is a major enzyme in the sterol biosynthetic pathway | Leishmania donovani |
physiological function | squalene synthase catalyses an unusual head-to-head reductive dimerization of two molecules of farnesyl-pyrophosphate in a two-step reaction to form squalene | Leishmania donovani |