BRENDA - Reference to 2.5.1.21; Id = 687416

NADPH

maximal activity at 2 mM

Thermosynechococcus elongatus

Tween 80

maximal activity at 2% v/v

Thermosynechococcus elongatus

expression in Escherichia coli, His-tagged protein found exclusively in inclusion bodies

Thermosynechococcus elongatus

zaragozic acid A

pH 7.2, 37°C

Thermosynechococcus elongatus

0.00097

-

farnesyl diphosphate

pH 7.2, 37°C

Thermosynechococcus elongatus

Mg2+

required

Thermosynechococcus elongatus

39900

-

x * 42000, SDS-PAGE, x * 41886, electrospray mass spectrometry, x * 39900, calculated

Thermosynechococcus elongatus

41886

-

x * 42000, SDS-PAGE, x * 41886, electrospray mass spectrometry, x * 39900, calculated

Thermosynechococcus elongatus

42000

-

x * 42000, SDS-PAGE, x * 41886, electrospray mass spectrometry, x * 39900, calculated

Thermosynechococcus elongatus

-

recombinant His-tagged protein found exclusively in inclusion bodies. Solubilization in presence of glycerol

Thermosynechococcus elongatus

-80°C, stable for at least 6 months

Thermosynechococcus elongatus

2 farnesyl diphosphate

-

687416

Thermosynechococcus elongatus

presqualene diphosphate + diphosphate

in absence of NADPH, formation of presqualene diphosphate

-

?

farnesyl diphosphate + NADPH + H+

-

687416

Thermosynechococcus elongatus

squalene + diphosphate + NADP+

sole product

-

?

x * 42000, SDS-PAGE, x * 41886, electrospray mass spectrometry, x * 39900, calculated

Thermosynechococcus elongatus

60

-

Thermosynechococcus elongatus

1.74

-

farnesyl diphosphate

-

Thermosynechococcus elongatus

7.6

-

Thermosynechococcus elongatus

0.0000955

-

pH 7.2, 37°C

Thermosynechococcus elongatus

zaragozic acid A

NADPH

maximal activity at 2 mM

Thermosynechococcus elongatus

Tween 80

maximal activity at 2% v/v

Thermosynechococcus elongatus

expression in Escherichia coli, His-tagged protein found exclusively in inclusion bodies

Thermosynechococcus elongatus

0.0000955

-

pH 7.2, 37°C

Thermosynechococcus elongatus

zaragozic acid A

pH 7.2, 37°C

Thermosynechococcus elongatus

0.00097

-

farnesyl diphosphate

pH 7.2, 37°C

Thermosynechococcus elongatus

Mg2+

required

Thermosynechococcus elongatus

39900

-

x * 42000, SDS-PAGE, x * 41886, electrospray mass spectrometry, x * 39900, calculated

Thermosynechococcus elongatus

41886

-

x * 42000, SDS-PAGE, x * 41886, electrospray mass spectrometry, x * 39900, calculated

Thermosynechococcus elongatus

42000

-

x * 42000, SDS-PAGE, x * 41886, electrospray mass spectrometry, x * 39900, calculated

Thermosynechococcus elongatus

recombinant His-tagged protein found exclusively in inclusion bodies. Solubilization in presence of glycerol

Thermosynechococcus elongatus

-80°C, stable for at least 6 months

Thermosynechococcus elongatus

2 farnesyl diphosphate

-

687416

Thermosynechococcus elongatus

presqualene diphosphate + diphosphate

in absence of NADPH, formation of presqualene diphosphate

-

?

farnesyl diphosphate + NADPH + H+

-

687416

Thermosynechococcus elongatus

squalene + diphosphate + NADP+

sole product

-

?

x * 42000, SDS-PAGE, x * 41886, electrospray mass spectrometry, x * 39900, calculated

Thermosynechococcus elongatus

60

-

Thermosynechococcus elongatus

1.74

-

farnesyl diphosphate

-

Thermosynechococcus elongatus

7.6

-

Thermosynechococcus elongatus

737480

Shiuan

Discovering peptide inhibitors ...

Homo sapiens

Appl. Biochem. Biotechnol.

179

597-609

2016

-

1

-

24

-

1

-

2

-

1

-

1

-

1

-

4

-

1

-

4

24

-

1

-

1

-

1

-

737775

Linscott

Mapping a kingdom-specific fun ...

Arabidopsis thaliana, Botryococcus braunii, Homo sapiens, Saccharomyces cerevisiae

Biochim. Biophys. Acta

1861

1049-1057

2016

-

4

-

1

-

4

-

10

-

4

-

4

-

1

-

4

-

4

-

6

-

739582

Zha

Cloning, prokaryotic expressio ...

Magnolia officinalis

Protein Expr. Purif.

120

28-34

2016

-

1

-

1

-

1

-

5

-

1

-

1

-

1

-

1

-

1

-

739735

Filiz

-

Comparative analyses of squale ...

Pinus massoniana, Populus trichocarpa

Tree Genet. Genomes

12

32

2016

-

2

-

2

-

2

-

8

2

-

2

-

2

-

2

-

2

-

8

2

-

2

-

6

-

738718

Katabami

Production of squalene by squa ...

Homo sapiens, Thermosynechococcus elongatus

J. Biosci. Bioeng.

119

165-171

2015

-

2

-

2

-

2

-

2

-

6

-

2

-

1

-

2

-

2

-

2

-

2

-

2

-

2

-

2

-

2

738798

Huang

Directed optimization of a new ...

Mortierella alpina, Mortierella alpina ATCC 32222

J. Ind. Microbiol. Biotechnol.

42

1341-1352

2015

-

1

-

1

-

3

-

1

2

-

6

-

1

-

2

1

-

1

-

1

-

1

-

1

-

3

-

1

2

-

1

-

2

1

-

1

-

3

-

1

738940

Saito

Targeting cellular squalene sy ...

Homo sapiens

J. Virol.

89

2220-2232

2015

-

1

-

1

-

2

-

3

-

2

-

1

-

1

-

1

-

2

-

2

-

1

-

739290

Patel

-

Overexpression of squalene syn ...

Withania somnifera

Plant Cell Tissue Organ Cult.

122

409-420

2015

-

1

-

1

-

2

-

1

-

1

-

1

-

4

-

1

-

1

-

1

-

1

-

1

-

2

-

1

-

1

-

4

-

1

-

1

-

2

-

739406

Kajikawa

Accumulation of squalene in a ...

Chlamydomonas reinhardtii

PLoS ONE

10

e0120446

2015

-

1

-

1

-

1

2

1

-

5

-

1

-

1

2

-

1

-

1

-

1

-

1

2

1

-

1

-

1

-

3

-

737345

Liu

Structural insights into the c ...

Homo sapiens

Acta Crystallogr. Sect. D

70

231-241

2014

-

1

3

-

1

-

3

-

4

-

2

-

1

-

6

-

1

-

1

-

1

3

-

1

-

3

-

4

-

1

-

6

-

2

-

738214

Ohtake

Biochemical characterization o ...

Methylococcus capsulatus

FEBS J.

281

5479-5497

2014

1

-

1

-

12

-

4

3

-

2

-

1

-

4

-

1

-

2

-

1

-

2

1

-

1

-

1

-

1

-

12

-

4

-

3

-

2

-

1

-

1

-

2

-

1

-

2

1

-

1

-

1

738235

Furubayashi

Directed evolution of squalene ...

Homo sapiens, Saccharomyces cerevisiae, Thermosynechococcus elongatus

FEBS Lett.

588

3375-3381

2014

-

3

-

4

-

7

-

4

-

3

-

4

-

3

-

739037

Sanchita

In silico study of binding mot ...

Withania somnifera

Mol. Biol. Rep.

41

7201-7208

2014

-

1

-

1

-

1

-

5

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

2

-

739589

Lee

Cloning, expression and purifi ...

Candida tropicalis, Candida tropicalis ATCC MYA-3404

Protein Expr. Purif.

94

15-21

2014

-

1

-

1

-

2

-

2

-

5

-

1

-

2

1

-

1

-

1

-

1

-

2

-

2

-

1

-

2

1

-

741903

Bell

Structure-function mapping of ...

Botryococcus braunii

Biochemistry

53

7570-7581

2014

-

1

-

19

-

1

-

2

-

4

-

4

-

1

-

1

-

1

-

1

-

19

-

1

-

2

-

4

-

1

-

1

-

2

-

722843

Grover

Enhanced withanolide productio ...

Withania somnifera

J. Biosci. Bioeng.

115

680-685

2013

-

1

-

4

-

4

-

1

-

4

-

1

-

723165

Kalra

Characterization of Squalene s ...

Chlorophytum borivilianum

Mol. Biotechnol.

54

944-953

2013

-

1

-

1

-

5

-

4

-

2

1

-

1

-

1

-

1

-

4

-

2

-

1

-

2

-

738856

Hong

Characterization of a squalene ...

Aurantiochytrium sp. KRS101

J. Microbiol. Biotechnol.

23

759-765

2013

-

1

-

1

-

1

-

3

-

1

-

2

1

-

1

-

1

-

1

-

1

-

1

-

1

-

2

1

-

1

-

2

-

721838

Ichikawa

Discovery of novel tricyclic c ...

Rattus norvegicus

Bioorg. Med. Chem.

20

3072-3093

2012

-

46

-

1

-

1

-

1

-

1

-

44

-

44

46

-

1

-

1

-

1

-

722107

Sun

Cloning and expression analysi ...

Solanum nigrum

Drug Discov. Ther.

6

242-248

2012

-

1

-

4

-

2

-

1

-

2

-

1

-

722759

Liu

Binding modes of zaragozic aci ...

Homo sapiens

J. Biol. Chem.

287

18750-18757

2012

-

1

-

1

-

1

-

3

-

1

-

3

-

1

-

1

-

1

-

1

-

1

-

1

-

3

-

2

-

723148

Gupta

Functional characterization an ...

Withania somnifera

Mol. Biol. Rep.

39

8803-8812

2012

-

1

-

1

3

-

8

-

1

-

4

-

3

1

-

1

-

1

-

1

-

1

3

-

1

-

4

-

3

1

-

1

-

2

-

723500

Ginzberg

Induction of potato steroidal ...

Solanum tuberosum, Solanum tuberosum Desiree

Planta

235

1341-1353

2012

-

1

-

2

-

4

-

2

-

2

-

1

-

1

-

2

-

2

-

721801

Griebenow

Identification and optimizatio ...

Homo sapiens

Bioorg. Med. Chem. Lett.

21

2554-2558

2011

-

21

-

1

-

1

-

1

-

20

-

20

21

-

1

-

1

-

721802

Griebenow

Identification of 4H,6H-[2]ben ...

Homo sapiens

Bioorg. Med. Chem. Lett.

21

3648-3653

2011

-

13

-

1

-

1

-

1

-

10

-

10

13

-

1

-

1

-

2

-

723497

Kim

Gene regulation patterns in tr ...

Bupleurum falcatum

Planta

233

343-355

2011

-

1

-

1

-

1

-

3

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

3

1

-

722293

Bhargava

Cloning, overexpression and ch ...

Leishmania donovani, Leishmania donovani MHOM/IN/80/Dd8

FEMS Microbiol. Lett.

311

82-92

2010

-

1

-

3

-

1

-

2

-

3

-

1

-

2

1

-

1

-

1

-

1

-

3

1

3

-

1

-

2

-

1

-

2

1

-

1

-

2

-

697143

Seiki

Pharmacologic inhibition of sq ...

Callithrix sp., Canis lupus familiaris, Cavia porcellus, Cricetus cricetus, Homo sapiens, Macaca sp., Mus musculus, Oryctolagus cuniculus, Rattus norvegicus

Cardiol. Rev.

17

70-76

2009

-

1

-

26

-

18

9

-

9

-

9

-

9

-

18

-

1

-

18

-

26

-

18

9

-

9

-

17

-

703278

Do

Squalene synthase: a critical ...

Cavia porcellus, Homo sapiens, Macaca mulatta, Mus musculus, Oryctolagus cuniculus, Rattus norvegicus

Clin. Genet.

75

19-29

2009

-

5

-

12

-

6

-

12

-

7

-

1

-

12

-

18

-

5

-

12

-

6

-

12

-

1

-

12

-

7

-

705303

Pan

Recombinant squalene synthase. ...

Saccharomyces cerevisiae

J. Org. Chem.

74

7562-7565

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-

3

-

1

-

1

-

3

1

-

1

-

1

-

1

-

1

-

1

-

706375

Uchida

Cloning and characterization o ...

Euphorbia tirucalli

Planta

229

1243-1252

2009

-

1

-

1

-

1

-

4

-

2

-

2

1

-

1

-

1

-

1

-

2

-

1

-

706801

Calo

-

Total synthesis and determinat ...

Homo sapiens

Tetrahedron Lett.

50

3388-3390

2009

-

1

-

2

-

1

-

2

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1

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2

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1

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684697

Bedi

Inhibition of squalene synthas ...

Rattus norvegicus

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470

116-119

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-

1

-

2

-

2

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1

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2

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685706

Paradise

Redirection of flux through th ...

Saccharomyces cerevisiae

Biotechnol. Bioeng.

100

371-378

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-

1

-

2

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1

-

1

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687043

Do

K45R variant of squalene synth ...

Homo sapiens

Hum. Mutat.

29

689-694

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1

-

1

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2

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1

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687416

Lee

Cloning, solubilization, and c ...

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J. Bacteriol.

190

3808-3816

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2

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1

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1

-

1

3

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9

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1

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1

2

1

-

1

-

1

-

1

2

-

1

-

1

-

1

-

1

3

-

1

-

1

2

1

-

1

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689567

Busquets

Arabidopsis thaliana contains ...

Arabidopsis thaliana

Plant Mol. Biol.

67

25-36

2008

-

1

-

1

-

1

-

9

-

4

-

2

-

1

-

1

-

4

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671377

Sealey-Cardona

Kinetic characterization of sq ...

Trypanosoma cruzi

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51

2123-2129

2007

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1

-

6

2

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1

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1

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1

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2

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1

-

6

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1

-

6

-

2

-

1

-

1

-

2

1

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673314

Charlton-Menys

Squalene synthase inhibitors: ...

Homo sapiens

Drugs

67

11-16

2007

-

6

-

2

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1

-

1

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1

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1

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6

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1

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1

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674822

Brusselmans

Squalene synthase, a determina ...

Homo sapiens

J. Biol. Chem.

282

18777-18785

2007

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1

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1

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1

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1

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1

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1

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1

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1

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1

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687473

Huang

Molecular cloning and characte ...

Taxus cuspidata

J. Biochem. Mol. Biol.

40

625-635

2007

-

1

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3

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2

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1

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1

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1

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2

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1

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1

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688310

Zhao

Cloning and characterization o ...

Ganoderma lucidum

J. Microbiol. Biotechnol.

17

1106-1112

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1

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5

-

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1

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688316

Choi

Isolation and structural deter ...

Sus scrofa

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17

1970-1975

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1

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Okazaki

Increased cholesterol biosynth ...

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47

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Orenes Lorente

Biphenylquinuclidines as inhib ...

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Bioorg. Med. Chem.

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3519-3529

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4

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8

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4

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660109

Seo

Overexpression of squalene syn ...

Panax ginseng

Phytochemistry

66

869-877

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-

1

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3

-

1

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658266

Ishihara

Synthesis and biological evalu ...

Homo sapiens, Rattus norvegicus

Bioorg. Med. Chem.

12

5899-5908

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1

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18

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Lee

Enhanced triterpene and phytos ...

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Plant Cell Physiol.

45

976-984

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1

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3

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2

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1

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1

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1

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658181

Akamine

cDNA cloning, mRNA expression, ...

Lotus japonicus

Biochim. Biophys. Acta

1626

97-101

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1

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4

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2

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489852

Devarenne

Regulation of squalene synthas ...

Nicotiana tabacum

Plant Physiol.

129

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3

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489850

Okada

Molecular characterization of ...

Botryococcus braunii

Arch. Biochem. Biophys.

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1

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1

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11

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Structure and regulation of ma ...

Homo sapiens, Rattus norvegicus, Saccharomyces cerevisiae

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3

2

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Pandit

Crystal structure of human squ ...

Homo sapiens

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275

30610-30617

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1

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Hayashi

Molecular cloning and characte ...

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Devarenne

Molecular characterization of ...

Nicotiana tabacum

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1

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1

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489849

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Truncation of human squalene s ...

Homo sapiens

Arch. Biochem. Biophys.

350

283-290

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1

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1

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4

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Gu

Function-structure studies and ...

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1

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-

Squalene synthase from cell su ...

Tabernaemontana divaricata

Phytochemistry

45

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1

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2

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Soltis

Expression, purification, and ...

Homo sapiens

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1

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Nakashima

Cloning, expression, and chara ...

Arabidopsis thaliana

Proc. Natl. Acad. Sci. USA

92

2328-2332

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1

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1

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9

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1

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1

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489860

Zhang

Biosynthesis of non-head-to-ta ...

Saccharomyces cerevisiae

J. Am. Chem. Soc.

117

1641-1642

1995

-

2

-

1

-

1

-

489853

Mookhtiar

Yeast squalene synthase. A mec ...

Saccharomyces cerevisiae

J. Biol. Chem.

269

11201-11207

1994

1

-

2

-

1

-

1

-

489838

Zhang

Yeast squalene synthase: expre ...

Saccharomyces cerevisiae

Arch. Biochem. Biophys.

304

133-143

1993

4

-

1

-

4

1

-

2

1

-

3

-

1

-

1

3

1

-

1

-

4

-

1

-

4

-

1

-

2

1

-

1

-

1

3

1

-

1

-

489839

LoGrasso

Overexpression, purification, ...

Saccharomyces cerevisiae

Arch. Biochem. Biophys.

307

193-199

1993

-

1

-

1

-

3

-

1

-

3

-

1

-

1

-

3

1

-

1

-

2

-

1

2

-

1

-

3

-

1

-

1

-

1

-

3

1

-

1

-

489834

McKenzie

Molecular cloning, expression, ...

Rattus norvegicus

J. Biol. Chem.

267

21368-21374

1992

-

1

-

1

-

1

-

3

-

2

-

3

-

1

-

1

-

1

-

2

-

3

-

489840

Ericsson

Isoprenoid biosynthesis in rat ...

Rattus norvegicus

J. Biol. Chem.

267

18708-18714

1992

1

-

1

-

2

-

2

-

2

1

-

3

-

1

-

1

-

2

-

2

1

-

3

-

489841

Hanley

Solubilization, partial purifi ...

Nicotiana tabacum

Plant Physiol.

98

215-220

1992

-

1

2

-

1

-

3

-

1

-

2

1

-

2

1

-

1

-

1

-

2

-

2

-

1

2

-

1

-

1

-

2

1

-

2

1

-

1

-

489842

Shechter

Solubilization, purification, ...

Rattus norvegicus

J. Biol. Chem.

267

8628-8635

1992

-

1

-

1

-

1

-

3

-

1

-

3

1

-

1

-

1

-

1

-

1

-

1

-

3

1

-

1

-

489835

Belingheri

Solubilization and partial pur ...

Narcissus pseudonarcissus

FEBS Lett.

292

34-36

1991

-

2

-

3

-

2

-

1

-

1

-

1

-

1

-

2

-

3

-

1

-

1

-

1

-

1

-

489832

Poulter

-

Squalene synthetase. Inhibitio ...

Saccharomyces cerevisiae

J. Am. Chem. Soc.

111

3734-3739

1989

-

1

-

1

-

1

-

2

-

1

-

1

-

1

-

2

-

489830

Sasiak

Purification to homogeneity an ...

Saccharomyces cerevisiae

Arch. Biochem. Biophys.

260

622-627

1988

-

2

-

1

-

2

-

1

-

1

-

3

1

-

1

-

1

-

2

-

2

-

1

-

1

-

1

-

3

1

-

489826

Kuswik-Rabiega

Squalene synthetase. Solubiliz ...

Saccharomyces cerevisiae

J. Biol. Chem.

262

1505-1509

1987

-

2

-

1

-

2

-

1

-

2

-

2

-

2

-

2

-

2

-

1

-

1

-

2

-

2

-

489827

Agnew

Squalene synthetase ...

Saccharomyces cerevisiae

Methods Enzymol.

110

359-373

1985

-

1

-

1

-

1

-

2

-

2

-

2

-

1

-

1

-

2

-

489828

Nishino

Formation of 12-cis-dehydrosqu ...

Saccharomyces cerevisiae

Methods Enzymol.

110

373-375

1985

-

1

2

-

1

-

2

-

1

-

1

-

1

2

-

2

-

489837

Takatsuji

Formation of dehydrosqualene c ...

Saccharomyces cerevisiae, Sus scrofa

J. Biochem.

91

911-921

1982

-

2

4

1

-

5

-

1

-

1

-

6

-

2

-

2

-

2

-

2

4

1

-

1

-

1

-

6

-

2

-

489836

Koyama

Substrate specificity of squal ...

Sus scrofa

Biochim. Biophys. Acta

617

218-224

1980

-

1

-

1

-

2

-

2

-

9

-

1

-

1

-

1

-

2

-

9

-

1

-

489845

Agnew

Squalene synthetase. Stoichiom ...

Saccharomyces cerevisiae

J. Biol. Chem.

253

4566-4573

1978

1

-

2

-

2

-

1

-

2

-

2

-

1

-

2

-

2

-

2

-

489846

Agnew

Squalene synthetase. Solubiliz ...

Saccharomyces cerevisiae

J. Biol. Chem.

253

4574-4583

1978

1

-

1

-

2

-

1

-

2

-

1

-

2

-

1

-

1

-

1

-

2

-

1

-

1

-

2

-

489831

Washburn

-

Investigations of substrate sp ...

Saccharomyces cerevisiae

Tetrahedron Lett.

18

1555-1558

1977

-

1

-

6

-

1

-

1

-

1

-

6

-

1

-

489833

Ortiz de Montellano

Substrate selectivity of squal ...

Rattus norvegicus, Saccharomyces cerevisiae

Biochemistry

16

2680-2685

1977

-

4

-

1

-

10

-

1

-

10

-

489829

Qureshi

Squalene synthetase. I. Dissoc ...

Saccharomyces cerevisiae

Biochem. Biophys. Res. Commun.

48

1123-1128

1972

-

1

-

1

-

3

-

1

-

1

-

1

-

3

-

489847

Dugan

Hog liver squalene synthetase: ...

Sus scrofa

Arch. Biochem. Biophys.

152

28-35

1972

-

1

-

1

-

1

-

1

-

2

-

2

-

1

-

1

-

1

-

489843

Shechter

Solubilization and purificatio ...

Saccharomyces cerevisiae

J. Biol. Chem.

246

7690-7696

1971

-

1

-

1

-

1

-

1

-

1

-

1

-

2

-

2

-

1

-

1

-

1

-

1

-

1

-

Cloning, solubilization, and characterization of squalene synthase from Thermosynechococcus elongatus BP-1

Data extracted from this reference: