Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli, several truncated versions of SQS with a His tag attached to the amino terminus | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(3R)-3-[[3-(benzyloxy)phenyl]ethynyl]-1-azabicyclo[2.2.2]octan-3-ol | IC50: 1500 nM | Trypanosoma cruzi | |
3-(biphenyl-4-ylmethyl)-1-azabicyclo[2.2.2]oct-2-ene | IC50: 730 nM | Trypanosoma cruzi | |
3-[[4-(benzyloxy)phenyl]ethynyl]-1-azabicyclo[2.2.2]oct-2-ene | IC50: 830 nM | Trypanosoma cruzi | |
E5700 | IC50: 0.84 nM | Trypanosoma cruzi | |
ER119884 | IC50: 3.52 nM | Trypanosoma cruzi | |
ethyl 4-(1-azabicyclo[2.2.2]oct-2-en-3-yl)benzoate | IC50: 50 nM | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00525 | - |
farnesyl diphosphate | in 50 mM morpholinepropanesulfonic acid-NaOH buffer (pH 7.4), 20 mM MgCl2, 5 mM 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate, 1% Tween 80, 10 mM dithiothreitol, 0.025 mg/ml bovine serum albumin, 0.25 mM NADPH, at 37°C | Trypanosoma cruzi | |
0.02334 | - |
NADPH | in 50 mM morpholinepropanesulfonic acid-NaOH buffer (pH 7.4), 20 mM MgCl2, 5 mM 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate, 1% Tween 80, 10 mM dithiothreitol, 0.025 mg/ml bovine serum albumin, 0.25 mM NADPH, at 37°C | Trypanosoma cruzi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41670 | - |
SDS-PAGE, estimated molecular mass | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HiTrap nickel-chelating column chromatography and MonoQ HR5/5 anion-exchange column chromatography | Trypanosoma cruzi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.1 | - |
truncated protein, soluble extract | Trypanosoma cruzi |
0.459 | - |
truncated protein, after 4.5fold purification | Trypanosoma cruzi |
Storage Stability | Organism |
---|---|
-80°C, 25 mM sodium phosphate (pH 7.4, containing 20 mM NaCl, 2 mM dithiothreitol, 1 mM EDTA, 40% glycerol, 10% methanol, 1 mM phenylmethylsulfonyl fluoride, 0.01 mM leupeptin, 0.001 mM pepstatin), extended periods of time, no loss of activity | Trypanosoma cruzi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
farnesyl diphosphate + NAD(P)H + H+ | - |
Trypanosoma cruzi | squalene + diphosphate + NAD(P)+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
farnesyl-diphosphate farnesyltransferase | - |
Trypanosoma cruzi |
SQS | - |
Trypanosoma cruzi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Trypanosoma cruzi |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00000084 | - |
IC50: 0.84 nM | Trypanosoma cruzi | E5700 | |
0.00000352 | - |
IC50: 3.52 nM | Trypanosoma cruzi | ER119884 | |
0.00005 | - |
IC50: 50 nM | Trypanosoma cruzi | ethyl 4-(1-azabicyclo[2.2.2]oct-2-en-3-yl)benzoate | |
0.00073 | - |
IC50: 730 nM | Trypanosoma cruzi | 3-(biphenyl-4-ylmethyl)-1-azabicyclo[2.2.2]oct-2-ene | |
0.00083 | - |
IC50: 830 nM | Trypanosoma cruzi | 3-[[4-(benzyloxy)phenyl]ethynyl]-1-azabicyclo[2.2.2]oct-2-ene | |
0.0015 | - |
IC50: 1500 nM | Trypanosoma cruzi | (3R)-3-[[3-(benzyloxy)phenyl]ethynyl]-1-azabicyclo[2.2.2]octan-3-ol |