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Literature summary for 2.5.1.20 extracted from

  • Takahashi, S.; Lee, H.; Yamashita, S.; Koyama, T.
    Characterization of cis-prenyltransferases from the rubber producing plant Hevea brasiliensis heterologously expressed in yeast and plant cells (2012), Plant Biotechnol., 29, 411-417.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
additional information recombinant HRT2, expressed in Escherichia coli, is significantly activated in the presence of a centrifuged latex fraction, resulting in the formation of polyisoprenes corresponding to natural rubber, but the recombinant HRT2 does not exhibit significant activity independently. HRT1/HRT2 require additional cofactors from the eukaryotic cells to produce distinct cPT activity, which seem to be contained in latex species Hevea brasiliensis

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of C-terminally His6-tagged HRT2 in Escherichia coli, expression of C-terminally His6-tagged HRT1 amd HRT2 in Saccharomyces cerevisiae and Arabidopsis thaliana T87 cultured cells, predominantly in the membrane fraction in yeast cells Hevea brasiliensis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Hevea brasiliensis 5737
-
additional information localization of the enzyme on small rubber particle and lutoid particles, which are considered to be polydispersed lysosomal vacuole Hevea brasiliensis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
x * 41000, recombinant C-terminally His6-tagged HRT1 or HRT2, SDS-PAGE Hevea brasiliensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
polycis-polyprenyl diphosphate + isopentenyl diphosphate Hevea brasiliensis
-
diphosphate + a polycis-polyprenyl diphosphate longer by one C5 unit
-
?

Organism

Organism UniProt Comment Textmining
Hevea brasiliensis
-
HRT1 and HRT2
-

Source Tissue

Source Tissue Comment Organism Textmining
latex
-
Hevea brasiliensis
-
laticifer
-
Hevea brasiliensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information recombinant HRT2 and HRT1 show distinct cPT activity producing polyisoprenoids with chain-lengths of C80-100, although failing to catalyze the formation of natural rubber. Recombinant HRT2, expressed in Escherichia coli, is significantly activated in the presence of a centrifuged latex fraction, resulting in the formation of polyisoprenes corresponding to natural rubber, but the recombinant HRT2 does not exhibit significant activity independently. The chain lengths of the HRT1/HRT2 products are not altered by the addition of centrifuged latex fractions, and the HRT1/HRT2 expressed in yeast compete with the rubber transferase activity of the latex fraction. Recombinantly produced polyprenyl products are apparently smaller than natural rubber molecular weight range Hevea brasiliensis ?
-
?
polycis-polyprenyl diphosphate + isopentenyl diphosphate
-
Hevea brasiliensis diphosphate + a polycis-polyprenyl diphosphate longer by one C5 unit
-
?

Subunits

Subunits Comment Organism
? x * 41000, recombinant C-terminally His6-tagged HRT1 or HRT2, SDS-PAGE Hevea brasiliensis

Synonyms

Synonyms Comment Organism
cisprenyltransferase
-
Hevea brasiliensis
CPT
-
Hevea brasiliensis
HRT1
-
Hevea brasiliensis
HRT2
-
Hevea brasiliensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Hevea brasiliensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Hevea brasiliensis

Cofactor

Cofactor Comment Organism Structure
additional information latex specific co-factor(s) may be required to enable HRT1/HRT2 rubber transferase activity. Recombinant HRT1/HRT2 require additional cofactors from the eukaryotic cells to produce distinct cPT activity Hevea brasiliensis

Expression

Organism Comment Expression
Hevea brasiliensis recombinant expression of HRT2 in Escherichia coli is significantly activated in the presence of a centrifuged latex fraction, resulting in the formation of polyisoprenes corresponding to natural rubber up

General Information

General Information Comment Organism
metabolism HRT2 is thought to be a key enzyme in the biosynthesis of natural rubber Hevea brasiliensis